Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,227,794 C→G 7.4% S211R (AGC→AGG)  PP_2828 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,227,7940CG 7.4% 169.3 / 4.5 54S211R (AGC→AGG) PP_2828hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (24/26);  new base G (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

CCGGAAGCCCTGCTGGGTTGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGC  >  NC_002947/3227648‑3227936
                                                                                                                                                  |                                                                                                                                              
gcGGATGTCCTGCTGGGTTGCCTCCTGTACCTGTATGTGTCGGGCGCCGTGGTTCAGGCGTTGCAGTCAGCGGTGCCTCCCGATGAGTTGTTCTCGGGCATGCCCGGTCTGCATCGGAGCAGATACATGTCTTGGGCGCTGAGCAGCCt                                                                                                                                              <  6:227696/148‑1 (MQ=255)
ccGGAAGCCCTGCTGGGTTGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCt                                                                                                                                              <  1:238574/149‑1 (MQ=255)
  ggAAGCCCTGCTGGGTTGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTgc                                                                                                                                            <  2:285888/149‑1 (MQ=255)
   gAAGCCCTGCTGGGTTGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTgcg                                                                                                                                           >  4:53890/1‑149 (MQ=255)
    aaGCCCTGCTGGGTTGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTgcgc                                                                                                                                          <  6:215751/149‑1 (MQ=255)
       cccTGCTGGGTTGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCAGGCGCAGGCTGCGccgg                                                                                                                                       >  3:50491/1‑147 (MQ=255)
         ctgctgGGTTGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGc                                                                                                                                     >  4:226688/1‑149 (MQ=255)
         ctgctgGGTTGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCAGGCGCAGGCTGCGCCGGGc                                                                                                                                     <  4:50491/149‑1 (MQ=255)
            ctgGGTTGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCAGGCGCAGGCTGCGCCGGGccc                                                                                                                                   <  4:269762/148‑2 (MQ=255)
               ggTTGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGc                                                                                                                                 <  3:221433/147‑1 (MQ=255)
               ggTTGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCg                                                                                                                                <  5:276195/148‑1 (MQ=255)
                 ttGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGc                                                                                                                                 <  6:215027/145‑1 (MQ=255)
                 ttGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGc                                                                                                                                 >  5:215027/1‑145 (MQ=255)
                    ccgccTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATg                                                                                                                          >  1:22642/1‑149 (MQ=255)
                          gTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCg                                                                                                                     >  2:100044/1‑148 (MQ=255)
                          gTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCg                                                                                                                     >  6:1809/1‑148 (MQ=255)
                                       gcgggcgCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCAc                                                                                                         >  4:263387/1‑147 (MQ=255)
                                         gggcgCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGt                                                                                                     >  3:17970/1‑149 (MQ=255)
                                           gcgcCTTGGTTCAGGCGTTGCATGGAGCGGTGCCTCGCGATCCGGTGGTCACCGCCATGCCCGGGCTCGAGCGGCTGCGATACCTGGCGGGGGCCGGGCGATGCCTGCTCCTGGCCGGCGTGCATGCACTGCATGCAGTGCACCAGTGc                                                                                                   <  8:299305/149‑1 (MQ=255)
                                           gcgcCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGc                                                                                                   <  3:226688/149‑1 (MQ=255)
                                                 tGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTggcg                                                                                             <  3:53890/149‑1 (MQ=255)
                                                                     gCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAg                                                                         <  1:100044/149‑1 (MQ=255)
                                                                          gCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGAt                                                                    <  5:208312/149‑1 (MQ=255)
                                                                          gCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGAt                                                                    >  1:208092/1‑149 (MQ=255)
                                                                           ccTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGAtt                                                                   <  4:179813/149‑1 (MQ=255)
                                                                             tCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTg                                                                  >  6:167152/1‑148 (MQ=255)
                                                                               ccGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCAc                                                               >  3:179238/1‑149 (MQ=255)
                                                                                cGATGCGTTGGTCACCGCAATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCAGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACg                                                              <  8:150250/149‑1 (MQ=255)
                                                                                          gTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTgg                                                    >  6:275223/1‑149 (MQ=255)
                                                                                             aCCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTggggg                                                 >  5:114284/1‑149 (MQ=255)
                                                                                             aCCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTgggg                                                  <  2:208092/148‑1 (MQ=255)
                                                                                             aCCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTgggg                                                  <  2:272132/148‑1 (MQ=255)
                                                                                                      cccGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCggg                                        <  2:186241/149‑1 (MQ=255)
                                                                                                         gggCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCgg                                         >  5:241895/1‑145 (MQ=255)
                                                                                                         gggCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCgg                                         <  6:241895/145‑1 (MQ=255)
                                                                                                          ggCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCGGATTGCACGGTGCTGTTGGGGGCGCACCGggtggt                                    <  5:1809/149‑1 (MQ=255)
                                                                                                               cAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGc                                <  2:22642/148‑1 (MQ=255)
                                                                                                               cAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCAACAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGTGCGCACCGGGTGGTCAGCa                               >  6:169454/1‑149 (MQ=255)
                                                                                                                 gcggcgCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCAGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCAc                              >  3:228796/1‑148 (MQ=255)
                                                                                                                  cggcgCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGAt                                                                    >  8:34819/1‑109 (MQ=255)
                                                                                                                  cggcgCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGAt                                                                    <  7:34819/109‑1 (MQ=255)
                                                                                                                   ggcgCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAg                           >  1:88415/1‑149 (MQ=255)
                                                                                                                          aTACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTc                    >  7:69642/1‑149 (MQ=255)
                                                                                                                           tACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTcc                   <  4:228796/149‑1 (MQ=255)
                                                                                                                            aCCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATTCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTc                    >  6:124250/1‑147 (MQ=255)
                                                                                                                              cTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAgg                >  3:182986/1‑149 (MQ=255)
                                                                                                                                 gCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCCTGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGgcg              <  8:207346/148‑1 (MQ=255)
                                                                                                                                 gCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGGGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGgcg              <  8:207349/148‑1 (MQ=255)
                                                                                                                                       gCCAGGCGCAGGCTGCGCCGGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTAcc       >  5:184957/1‑149 (MQ=255)
                                                                                                                                        cccGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCg      <  5:167152/149‑1 (MQ=255)
                                                                                                                                        cccGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCg      >  3:268657/1‑149 (MQ=255)
                                                                                                                                            gcgcAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGCGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGc  >  6:32661/1‑149 (MQ=255)
                                                                                                                                            gcgcAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCACTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTAc        <  7:132412/143‑1 (MQ=255)
                                                                                                                                            gcgcAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCACTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTAc        >  8:132412/1‑143 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CCGGAAGCCCTGCTGGGTTGCCGCCTGTACCTGGAGCTGGCGGGCGCCGTGGTTCAGGCGTTGCAGGCAGCGGTGCCTCCCGATGCGTTGGTCACCGCCATGCCCGGGCTCCAGCGGCGCCGATACCTGGCGTGGGCCCGGCGCAGCCTGCGCCTGGCCGGCGTGCATGCCCCGCATGCAGTGCACCAGTGCCTGGCGGCGCTGGCCCAAGGGCTGAGCCGATTGCACGGTGCTGTTGGGGGCGCACCGGGTGGTCAGCACGAGCCGGTTCCAGGCGCCGTACCGGAGC  >  NC_002947/3227648‑3227936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑