Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,295,912 G→A 7.5% L74L (CTG→TTG)  PP_2896 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,295,9120GA 7.5% 149.6 / 2.1 53L74L (CTG→TTG) PP_2896hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base A (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

CTGGCGCCGGATGTTGCCGATCTGCCGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGCCTGCCGCCGTAGGCGCAGGTCAGCTGGTAGGCGTTTTCGATGCC  >  NC_002947/3295769‑3296056
                                                                                                                                                |                                                                                                                                                
cTGGCGCCGGATGTTGCCGATCTGCCGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGa                                                                                                                                             <  5:816/149‑1 (MQ=255)
       cGGATGTTGCCGATCTGCCGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCCTGGAGATCCCCAAGGctga                                                                                                                                            <  4:55439/143‑4 (MQ=255)
       cGGATGTTGCCGATCTGCCGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCCTGGAGATCCCCAAGGctga                                                                                                                                            >  3:55439/1‑140 (MQ=255)
           tGTTGCAGATCTGCCGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGAGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGAAGAAAGTGGTCAGCCTTGGGGATCTCCAGTGCGACCCCGGCAAAg                                                                                                                                  >  6:210798/1‑149 (MQ=255)
             ttGCCGATCTGCCGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAgcg                                                                                                                                >  5:16938/1‑149 (MQ=255)
                ccGATCTGCCGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCCTGGAGATCCCCAAGGctgacc                                                                                                                                          <  4:101085/136‑6 (MQ=255)
                ccGATCTGCCGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCCTGGAGATCCCCAAGGctgacc                                                                                                                                          >  3:101085/1‑131 (MQ=255)
                        ccGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGa                                                                                                                           <  6:239096/143‑1 (MQ=255)
                        ccGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATc                                                                                                                         <  3:230129/145‑1 (MQ=255)
                        ccGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGt                                                                                                                      <  7:47357/148‑1 (MQ=255)
                         cGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAACTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCtt                                                                                                                             >  1:164673/1‑140 (MQ=255)
                         cGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAACTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCtt                                                                                                                             <  2:164673/140‑1 (MQ=255)
                              gCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGa                                                                                                               <  6:329102/149‑1 (MQ=255)
                                tCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAgc                                                                                                             >  6:301099/1‑149 (MQ=255)
                                tCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAgc                                                                                                             >  3:155112/1‑149 (MQ=255)
                                 cAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGc                                                                                                                    <  8:297976/141‑1 (MQ=255)
                                 cAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGc                                                                                                                    >  7:297976/1‑141 (MQ=255)
                                      cGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGAGTCGAGCGCTTcg                                                                                                       >  5:92719/1‑149 (MQ=255)
                                          cTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTcggcc                                                                                                    <  2:135972/148‑1 (MQ=255)
                                             gttgGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTTGGCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTcggccgg                                                                                                  >  1:252387/1‑148 (MQ=255)
                                             gttgGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTcggccgg                                                                                                  >  3:174344/1‑147 (MQ=255)
                                                     gcagGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGTGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCa                                                                                        <  6:57701/149‑1 (MQ=255)
                                                       agGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGc                                                                                      <  6:16938/149‑1 (MQ=255)
                                                           tGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGc                                                                                  >  1:123979/1‑149 (MQ=255)
                                                             cGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCtt                                                                                <  4:174344/149‑1 (MQ=255)
                                                                gACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCa                                                                                                                        <  4:261253/106‑1 (MQ=255)
                                                                gACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCa                                                                                                                        >  3:261253/1‑106 (MQ=255)
                                                                gACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGt                                                                             <  2:252387/149‑1 (MQ=255)
                                                                 aCAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGt                                                                             >  1:280663/1‑148 (MQ=255)
                                                                 aCAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGt                                                                             <  2:280663/148‑1 (MQ=255)
                                                                       cGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTcg                                                                      >  6:117631/1‑149 (MQ=255)
                                                                           ggCGCGTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGGGCGGCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCgg                                                                   <  7:129304/148‑1 (MQ=255)
                                                                                   ccTTCTGGAGCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGAGCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTggg                                                           <  8:58261/148‑1 (MQ=255)
                                                                                     ttCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTg                                                                               <  1:2986/126‑1 (MQ=255)
                                                                                     ttCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTg                                                                               >  2:2986/1‑126 (MQ=255)
                                                                                     ttCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGAt                                                        >  5:312422/1‑149 (MQ=255)
                                                                                     ttCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGAt                                                        >  8:257561/1‑149 (MQ=255)
                                                                                     ttCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGAt                                                        >  7:36385/1‑149 (MQ=255)
                                                                                          gATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGt                                                      <  7:257561/146‑1 (MQ=255)
                                                                                            tCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTg                                                 >  5:232090/1‑149 (MQ=255)
                                                                                                ctgGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCTGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGc                                              <  5:210798/148‑1 (MQ=255)
                                                                                                ctgGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTg                                                 <  6:312422/145‑1 (MQ=255)
                                                                                                 tgGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGCCt                                            >  6:214559/1‑149 (MQ=255)
                                                                                                   gTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGCCTgc                                          >  1:202828/1‑149 (MQ=255)
                                                                                                        cgcAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGCCTgccgcc                                      <  2:123979/149‑1 (MQ=255)
                                                                                                         gcAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGCCTGccgccg                                     <  6:92719/149‑1 (MQ=255)
                                                                                                               gACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGCCTGCCGCCGTAGgcg                               >  5:185293/1‑149 (MQ=255)
                                                                                                                aCGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGc                                              <  4:162385/133‑1 (MQ=255)
                                                                                                                aCGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGc                                              >  3:162385/1‑133 (MQ=255)
                                                                                                                aCGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGCCTGCCGCCGTAGgcg                               <  5:301099/148‑1 (MQ=255)
                                                                                                                  gCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGCCTGCCGCCGTAGGCGCAg                            <  2:202828/149‑1 (MQ=255)
                                                                                                                                 ccTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGCCTGCCGCCGTAGGCGCAGGTCAGCTGGTAGGCg             >  5:56937/1‑149 (MQ=255)
                                                                                                                                             ccAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGCCTGCCGCCGTAGGCGCAGGTCAGCTGGTAGGCGTTTTCGATGcc  >  1:293687/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTGGCGCCGGATGTTGCCGATCTGCCGCAGGCTCAGGCCGTACTTGTTGGCCAGCAGGCTGCGGGACAAACCGTTGGCGCTTTCCTTCTGGATCTGCTGGTT‑GCGCAGCTGACGCAGAAAGTGGTCAGCCTTGGGGATCTCCAGGGCGACCCCGGCAAAGCGCTTGATCAGTGCGTCGAGCGCTTCGGCCGGCAGCACGTCAGCGAGCTTGGTGCGCTCGCGGTGCTTGGGGATGTACTTGGGCCTGCCGCCGTAGGCGCAGGTCAGCTGGTAGGCGTTTTCGATGCC  >  NC_002947/3295769‑3296056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑