Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,300,859 A→T 6.0% D16E (GAT→GAA)  lpxH ← UDP‑2,3‑diacylglucosamine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,300,8590AT 6.0% 202.6 / 2.6 67D16E (GAT→GAA) lpxHUDP‑2,3‑diacylglucosamine hydrolase
Reads supporting (aligned to +/- strand):  ref base A (32/31);  new base T (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

CGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGAC  >  NC_002947/3300716‑3301001
                                                                                                                                               |                                                                                                                                              
cGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                           <  5:211034/149‑1 (MQ=255)
     cATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGtt                                                                                                                                      <  5:116557/149‑1 (MQ=255)
         gCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttctt                                                                                                                                   <  8:103274/148‑1 (MQ=255)
              gCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCTAGGATGTACAGGGCCTTTGCATGGCGGGCGCGGCCATCGAGCAGATCAAGATACGCCCGGGAAATATCCGGGCGTTCTTCTTg                                                                                                                               <  2:231748/147‑1 (MQ=255)
                     gACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCa                                                                                                                       <  7:7401/148‑1 (MQ=255)
                        tgctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGtttct                                                                                                                                   <  1:21536/133‑4 (MQ=255)
                        tgctgGAAGGGCGTCATGGCATAGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGtttct                                                                                                                                   >  2:21536/1‑130 (MQ=255)
                          ctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagat                                                                                                                    >  6:159063/1‑146 (MQ=255)
                           tgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatca                                                                                                                  >  5:26900/1‑147 (MQ=255)
                             gAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCggatcagag                                                                                                               <  3:14158/148‑1 (MQ=255)
                              aaGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatcagagat                                                                                                             <  2:191969/149‑1 (MQ=255)
                                     tCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATcagcag                                                                                                       >  4:227982/1‑148 (MQ=255)
                                        tGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAAt                                                                                                    >  1:179151/1‑148 (MQ=255)
                                         ggCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGCCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCa                                                                                                  >  7:137331/1‑149 (MQ=255)
                                          gCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCa                                                                                                  <  2:179151/148‑1 (MQ=255)
                                               gtcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCaa                                                                                             <  8:58044/148‑1 (MQ=255)
                                                tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAAt                                                                                            >  3:35309/1‑148 (MQ=255)
                                                  gCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCa                                                                                              >  8:124993/1‑144 (MQ=255)
                                                   ccAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGAtc                                                                                        >  4:120859/1‑149 (MQ=255)
                                                   ccAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGAt                                                                                         >  8:136430/1‑148 (MQ=255)
                                                     aaTCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGAtctc                                                                                      <  2:27703/149‑1 (MQ=255)
                                                          aCGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAATCGCCGGGGAAATATCCGGTCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCTGCtt                                                                                 <  5:223555/149‑1 (MQ=255)
                                                           cGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCttt                                                                                >  4:132581/1‑149 (MQ=255)
                                                             cTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTtct                                                                              <  5:159063/149‑1 (MQ=255)
                                                              ttCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTtctc                                                                             >  7:135834/1‑149 (MQ=255)
                                                              ttCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTtctc                                                                             >  1:326560/1‑149 (MQ=255)
                                                                 gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCgg                                                                                    <  2:323737/139‑1 (MQ=255)
                                                                 gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCgg                                                                                    >  1:323737/1‑139 (MQ=255)
                                                                 gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgat                                                                          >  3:114648/1‑149 (MQ=255)
                                                                         cACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGc                                                                                                                        <  4:59024/95‑1 (MQ=255)
                                                                         cACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGc                                                                                                                        >  3:59024/1‑95 (MQ=255)
                                                                          aCCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtc                                                                 >  5:152513/1‑149 (MQ=255)
                                                                          aCCAAGGATGTACAGGGCCTTGGCATGGAGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtc                                                                 >  1:186182/1‑149 (MQ=255)
                                                                           ccAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatcagagatca                                                                                                           >  2:221343/1‑106 (MQ=255)
                                                                           ccAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCagatcagagatca                                                                                                           <  1:221343/106‑1 (MQ=255)
                                                                           ccAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtcg                                                                >  5:292659/1‑149 (MQ=255)
                                                                            cAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtcgt                                                               >  8:11961/1‑149 (MQ=255)
                                                                            cAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtcgt                                                               >  1:66068/1‑149 (MQ=255)
                                                                                gATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCtt                                                           >  6:331162/1‑149 (MQ=255)
                                                                                  tGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGTGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCg                                                         <  3:120859/149‑1 (MQ=255)
                                                                                  tGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGTGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCg                                                         <  7:245392/149‑1 (MQ=255)
                                                                                    tACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGTAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGt                                                       <  4:46865/149‑1 (MQ=255)
                                                                                    tACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCgg                                                        <  7:124993/148‑1 (MQ=255)
                                                                                     aCAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCa                                                                                              >  1:261861/1‑109 (MQ=255)
                                                                                     aCAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCa                                                                                              <  2:261861/109‑1 (MQ=255)
                                                                                      cAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTAc                                                     >  7:259086/1‑149 (MQ=255)
                                                                                         ggCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTc                                                  <  6:39115/149‑1 (MQ=255)
                                                                                           ccTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCt                                                >  5:114120/1‑149 (MQ=255)
                                                                                            cTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTc                                                          <  1:203550/138‑1 (MQ=255)
                                                                                            cTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTc                                                          >  2:203550/1‑138 (MQ=255)
                                                                                            cTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCtg                                               <  5:331162/149‑1 (MQ=255)
                                                                                               ggCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCtggt                                             <  6:292659/148‑1 (MQ=255)
                                                                                                 cATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGc                                          >  8:180480/1‑149 (MQ=255)
                                                                                                 cATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTACAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTACTTCGGTACGTCCTGGTGGc                                          >  6:227515/1‑149 (MQ=255)
                                                                                                  aTGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTACGTACGTCCTGGTGGc                                          >  6:163188/1‑148 (MQ=255)
                                                                                                      cgggcgCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCGCGTCGTCCTTCGGTACGTCCTGGTGGCCAGCt                                     <  3:132581/149‑1 (MQ=255)
                                                                                                         gcgcGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTg                                  <  2:66068/149‑1 (MQ=255)
                                                                                                               ccATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCg                            <  4:35309/149‑1 (MQ=255)
                                                                                                                            cAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCCCGTCGTCCTTCGCTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttttt               <  8:137331/149‑1 (MQ=255)
                                                                                                                            agaagaaCGCCCGGATATTTCCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttttt               >  2:306118/6‑149 (MQ=255)
                                                                                                                              aGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTc              >  7:68838/1‑148 (MQ=255)
                                                                                                                                aaaCGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGAt           >  6:115476/1‑149 (MQ=255)
                                                                                                                                aaaCGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGAt           >  7:290507/1‑149 (MQ=255)
                                                                                                                                 aaCGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATg          <  4:256402/149‑1 (MQ=255)
                                                                                                                                      ccGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTc     <  5:115476/149‑1 (MQ=255)
                                                                                                                                        gggAAATATCCGGGCGTTCTTCTTGCAGGTCCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGTCATTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGa   <  5:227515/149‑1 (MQ=255)
                                                                                                                                         ggAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGAc  <  4:114648/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCAGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGAC  >  NC_002947/3300716‑3301001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑