Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,332,880 C→A 9.5% L75L (CTG→CTT)  PP_2930 ← L‑serine dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,332,8800CA 9.5% 113.6 / 2.0 39L75L (CTG→CTT) PP_2930L‑serine dehydratase
Reads supporting (aligned to +/- strand):  ref base C (19/16);  new base A (2/2);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

GTTCGGGCCTACCAGTGTCTGTGCCAGGGCGTTGGCCTCTTGCCAGGAACTGCCTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTGAACGAACCGCAGGGTTGCAGGGCGTCGAGCT  >  NC_002947/3332736‑3333028
                                                                                                                                                |                                                                                                                                                    
gTTCGGGCCTACCAGTGTCTGTGCCAGGGCGTTGGCCTCTTGCCAGGAACTGCCTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGc                                                                                                                                                   <  2:93347/148‑1 (MQ=255)
 ttCGGGCCTACCAGTGTCTGTGCCAGGGCGTTGGCCTCTTGCCAGGAACTGCCTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCtt                                                                                                                                                 <  8:94335/149‑1 (MQ=255)
 ttCGGGCCTACCAGTGTCTGTGCCAGGGCGTTGGCCTCTTGCCAGGAACTGCCTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCtt                                                                                                                                                 <  5:178968/149‑1 (MQ=255)
 ttCGGGCCTACCAGTGTCTGTGCCAGGGCGTTGGCCTCTTGCCAGGAACTGCCTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCtt                                                                                                                                                 <  4:252804/149‑1 (MQ=255)
 ttCGGGCCTAACAGTGTCTGTGACAGGGCGTTGGCCTCTTGCCAGGAACTGCCTTGCACCACCACCTTGTCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTACGGGTATCACCACGGTCAACGTCACCCCCAGCtt                                                                                                                                                 >  6:11401/1‑149 (MQ=255)
                      gCCAGGGCGTTGGCCTCTTGCCAGGAACTGCCTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGc                                                                                                                            >  4:9484/1‑149 (MQ=255)
                       ccAGGGCGTTGGCCTCTTGCCAGGAACTGCCTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGc                                                                                                                            >  3:153464/1‑148 (MQ=255)
                                         gCCAGGAACTGCCTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCTGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCATCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCAcc                                                                                                          <  5:11401/148‑1 (MQ=255)
                                          ccAGGAACTGCCTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCAAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCt                                                                                                         >  2:273565/1‑148 (MQ=255)
                                          ccAGGAACTGCCTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCAAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCt                                                                                                         >  2:277163/1‑148 (MQ=255)
                                                    ccTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGaca                                                                                               >  6:326291/1‑148 (MQ=255)
                                                    ccTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCATGGCCCGTTCGGTGGTCGTTTCGGGTAACACCACGGTCACCGGCACCCCCAGCTTGCGTCCGTCATAGGCGACAGCCACACACGCATTGCCACCTGACGactaca                                                                                               >  8:300349/1‑143 (MQ=255)
                                                            caccaccTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGcc                                                                                      >  6:107914/1‑149 (MQ=255)
                                                             accaccTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCg                                                                                     >  1:23631/1‑149 (MQ=255)
                                                                 ccTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGgcg                                                                                 >  8:123756/1‑149 (MQ=255)
                                                                         tCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCAcgcgc                                                                         >  6:245133/1‑149 (MQ=255)
                                                                             ccAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCgtg                                                                      <  3:298131/148‑1 (MQ=255)
                                                                             ccAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCgtg                                                                      <  4:153464/148‑1 (MQ=255)
                                                                                ggTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCgtg                                                                      >  2:289548/1‑145 (MQ=255)
                                                                                ggTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCgtg                                                                      <  1:289548/145‑1 (MQ=255)
                                                                                      gcaACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCg                                                            <  5:245133/149‑1 (MQ=255)
                                                                                          cTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGACAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAgg                                                        >  3:39292/1‑149 (MQ=255)
                                                                                                ggCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTg                                                    <  2:23631/147‑1 (MQ=255)
                                                                                                ggCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTg                                                    <  5:326291/147‑1 (MQ=255)
                                                                                                 gCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGcc                                                 <  5:107914/149‑1 (MQ=255)
                                                                                                  cccGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGcc                                                 >  3:274331/1‑148 (MQ=255)
                                                                                                  cccGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCAAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCATGGCCt                                                <  1:273565/149‑1 (MQ=255)
                                                                                                  cccGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCAAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCATGGCCt                                                <  1:277163/149‑1 (MQ=255)
                                                                                                     gTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTaca                                             >  1:167590/1‑149 (MQ=255)
                                                                                                           tggtggtTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAAACGGCATTGCCACCTGACGAGGAAACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACcgcg                                        >  6:103732/1‑148 (MQ=255)
                                                                                                                gtTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGttt                                  >  5:141518/1‑149 (MQ=255)
                                                                                                                 tttCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTg                                 <  7:123756/149‑1 (MQ=255)
                                                                                                                    cGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTgaac                              >  5:287401/1‑149 (MQ=255)
                                                                                                                             ccacGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTGAACGAACCGCAg                     >  1:84783/1‑149 (MQ=255)
                                                                                                                                     aCCGGCACCCCCAGGTTGCGTCGGTCATAGGCGACAGCCAAACCGGGATTGCCACCTGTCGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTGAACGAACCGCTGGGTTGCa              <  7:300349/148‑1 (MQ=255)
                                                                                                                                       cGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTGAACGAACCGCAGGGTTGCAggg           >  2:323104/1‑149 (MQ=255)
                                                                                                                                       cGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTGAACGAACCGCAGGGTTGCAggg           <  2:167590/149‑1 (MQ=255)
                                                                                                                                         gCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTGAACGAACCGCAGGGTTGCAGGGCg         >  5:234866/1‑149 (MQ=255)
                                                                                                                                           aCCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTGAACGAACCGCAGGGTTGCAGGGCGTc       <  6:119941/149‑1 (MQ=255)
                                                                                                                                             ccccAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCAcgcgc                                                                         <  8:79149/81‑1 (MQ=255)
                                                                                                                                             ccccAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTAc                                                                >  2:280942/1‑90 (MQ=255)
                                                                                                                                             ccccAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGACACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGCTGTACCTCGCAGGCGTGGCCTACAACGCGCAGTTTGAACGAACCGCAGGGTTGCAGGGCGTCGa     >  4:88715/1‑149 (MQ=255)
                                                                                                                                               ccAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTGAACGAACCGCAGGGTTGCAGGGCGTCGAGc   <  4:274331/149‑1 (MQ=255)
                                                                                                                                               ccAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTGAACGAACCGCAGGGTTGCAGGGCGTCGAGc   >  1:68426/1‑149 (MQ=255)
                                                                                                                                                cAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTGAACGAACCGCAGGGTTGCAGGGCGTCGAGCt  >  5:34903/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GTTCGGGCCTACCAGTGTCTGTGCCAGGGCGTTGGCCTCTTGCCAGGAACTGCCTTGCACCACCACCTTGGCGTCTTCCAGGTGCAGCAACTGCTTGGCCCGTTCGGTGGTGGTTTCGGGTATCACCACGGTCACCGGCACCCCCAGCTTGCGTCCGGCATAGGCGACTGCCAAACCGGCATTGCCACCTGACGAGGACACAAAGTGCCGGGCGCCACGCGCGTGGTGTACCTCGCAGGCGTGGCCTACACCGCGCAGTTTGAACGAACCGCAGGGTTGCAGGGCGTCGAGCT  >  NC_002947/3332736‑3333028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑