Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,418,519 G→A 9.5% intergenic (‑108/+51) prtN ← / ← PP_3032 transcriptional regulator PrtN/DNA‑binding protein Roi‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,418,5190GA 9.5% 115.1 / 2.4 43intergenic (‑108/+51)prtN/PP_3032transcriptional regulator PrtN/DNA‑binding protein Roi‑like protein
Reads supporting (aligned to +/- strand):  ref base G (21/17);  new base A (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01

CGCAGCGCGATTTGATTGGTATTGCTCATCAGGCTTCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCTGCTAGCCAGGGGATCCCGGCCTGCTTCACGCGTGTC  >  NC_002947/3418383‑3418663
                                                                                                                                        |                                                                                                                                                
cgcAGCGCGATTTGATTGGTATTGCTCATCAGGCTTCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGctcct                                                                                                                                      <  6:112519/149‑1 (MQ=255)
 gctgcgcGAGTTGATTGGTTTTGCTCATCAGGCTTCGGGCTCTCGCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAACGCTGCGctccag                                                                                                                                     <  4:208938/146‑3 (MQ=255)
 gcAGCGCGATTTGATTGGTATTGCTCATCAGGCTTCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAACGCTGCGctccag                                                                                                                                     <  6:190263/149‑3 (MQ=255)
              aTTGGTATTGCTCATCAGGCTTCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAAc                                                                                                                        >  8:39552/1‑149 (MQ=255)
                 ggTATTGGTGATCAGGCTTCGGGCTCTGCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTg                                                                                                                      <  4:226131/148‑1 (MQ=255)
                  gTATTGCTCATCAGGCTTCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTgg                                                                                                                     <  8:285186/148‑1 (MQ=255)
                  gTATTGCTCATCAGGCTTCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTgg                                                                                                                     <  5:241878/148‑1 (MQ=255)
                  gTATTGCTCATCAGGCTTCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTg                                                                                                                      >  4:91184/1‑147 (MQ=255)
                       gCTCATCAGGCTTCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATc                                                                                                               >  2:70569/1‑149 (MQ=255)
                         tcatcaGGCTTCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTg                                                                                                             >  6:178699/1‑149 (MQ=255)
                           atcaGGCTTCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTggg                                                                                                           >  2:225593/1‑149 (MQ=255)
                           atcaGGCTTCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTgg                                                                                                            <  2:222067/148‑1 (MQ=255)
                                  ttCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGGACTGGAGGATCTGGGCCGCGTa                                                                                                    >  5:281143/1‑149 (MQ=255)
                                     gggCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACt                                                                                                  <  2:156608/148‑1 (MQ=255)
                                     cgttcttcCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGCATCTGGGCCGCGTACTg                                                                                                 <  1:308673/143‑1 (MQ=255)
                                            cccTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTg                                                                                                 >  2:308673/1‑142 (MQ=255)
                                                         gCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCCCCATTCGTCAGGAACCTCCTGGAGCGCAGCGATGCGCTCCTCACGGGTTTTGAACTGCAGGATCTCGGCCGCGTACTGCCTAGGCCGCATAGCGATTg                                                                             >  7:129624/1‑149 (MQ=255)
                                                            tGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGt                                                                                                     <  6:65622/122‑1 (MQ=255)
                                                                   tGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGACCCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCggtg                                                                   <  5:229381/149‑1 (MQ=255)
                                                                         cAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCg                                                             <  5:178699/149‑1 (MQ=255)
                                                                               aTCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGt                                                        <  8:193235/148‑1 (MQ=255)
                                                                                  tcGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGATTGTCTTCCGGTGGTGTCGGAAGTTg                                                      >  2:187072/1‑147 (MQ=255)
                                                                                   cGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCGCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAg                                                   <  2:212177/149‑1 (MQ=255)
                                                                                    gCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTg                                                      <  3:91184/145‑1 (MQ=255)
                                                                                    gCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAgg                                                  >  4:74618/1‑149 (MQ=255)
                                                                                          cTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGGACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCgag                                             <  6:281143/148‑1 (MQ=255)
                                                                                           ttGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGAc                                           >  4:158110/1‑149 (MQ=255)
                                                                                             gCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGt                                         >  6:139642/1‑149 (MQ=255)
                                                                                                 aCCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCTg                                     >  4:112457/1‑149 (MQ=255)
                                                                                                  ccAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCggtg                                                                   <  7:122692/118‑1 (MQ=255)
                                                                                                  ccAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCggtg                                                                   >  8:122692/1‑118 (MQ=255)
                                                                                                   cAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGCTCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAACTTGCAGGTCGAGACGTTCTGc                                    >  7:229528/1‑148 (MQ=255)
                                                                                                    aGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCTGCTa                                  <  1:225593/149‑1 (MQ=255)
                                                                                                                  ttcAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCTGCTAGCCAGGGGATCCCg                    >  2:142831/1‑149 (MQ=255)
                                                                                                                     aGGAACCTCCTGGAGCGCATCGTTGCGCTCCTCACGTGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCTGCTAGCCAGCGGATCCCGGcc                 <  8:129624/149‑1 (MQ=255)
                                                                                                                     aGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCTGCTAGCCAGGGGATCCCGGcc                 >  6:122107/1‑149 (MQ=255)
                                                                                                                           ctcctGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCTGCTAGCCAGGGGATCCCg                    >  4:6319/1‑140 (MQ=255)
                                                                                                                           ctcctGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCTGCTAGCCAGGGGATCCCg                    <  3:6319/140‑1 (MQ=255)
                                                                                                                             tgaggAGCGCAACGCTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCTGCTAGCCAGGGGATCCCGGCCTGCTTCAc         >  5:320940/4‑149 (MQ=255)
                                                                                                                                ggAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCTGCTAGCCAGGGGATCCCGGCCTGCTTCAcgcg      >  7:132290/1‑149 (MQ=255)
                                                                                                                                 gAGCGCAACGCTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCTGCTAGCCAGGGGATCCCGGCCTGCTTCACGCgt     >  2:257167/1‑149 (MQ=255)
                                                                                                                                     gcAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGCCGTTCCGCTAGCCAGGGGATCCCGGCCTGCTTCACGCGTGTc  >  2:308823/1‑148 (MQ=255)
                                                                                                                                     gcAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCAGCTAGCCAGGGGATCCCGGCCTGCTTCACGCGTGTc  >  2:304072/1‑148 (MQ=255)
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CGCAGCGCGATTTGATTGGTATTGCTCATCAGGCTTCGGGCTCTCCCTGAGCTTGTGGCGTGACGGATGGTGCCAAGTGATCTCGCAGTGCTTGCGTACCAGGTCGCGCCATTCTTCAGGAACCTCCTGGAGCGCAGCGTTGCGCTCCTCACGGGTTTTGAACTGGAGGATCTGGGCCGCGTACTGCCTAGGCCGCATAGCGCTTGTCTTCCGGTGGTGTCGGAAGTTGCAGGTCGAGACGTTCTGCTAGCCAGGGGATCCCGGCCTGCTTCACGCGTGTC  >  NC_002947/3418383‑3418663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑