Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,435,922 G→A 10.6% Q278Q (CAG→CAA)  gpJ → baseplate/tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,435,9220GA10.6% 126.3 / 3.9 47Q278Q (CAG→CAA) gpJbaseplate/tail fiber protein
Reads supporting (aligned to +/- strand):  ref base G (17/25);  new base A (2/3);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAGGTGGTGA  >  NC_002947/3435776‑3436064
                                                                                                                                                  |                                                                                                                                              
ccccGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGtt                                                                                                                                              >  3:9962/1‑149 (MQ=255)
ccccGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGtt                                                                                                                                              >  1:36043/1‑149 (MQ=255)
    gcgTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGccc                                                                                                                                          <  5:78379/149‑1 (MQ=255)
          ccgccgGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAACTGCCCCTgcag                                                                                                                                    <  1:217226/149‑4 (MQ=255)
          ccgccgGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAACTGCCCCTgcag                                                                                                                                    <  7:248508/149‑4 (MQ=255)
          ccgccgGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTCAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCg                                                                                                                                    <  7:178943/149‑1 (MQ=255)
               ggCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCAt                                                                                                                               <  8:235958/149‑1 (MQ=255)
                 cGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCt                                                                                                                             <  2:132803/149‑1 (MQ=255)
                  gTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTa                                                                                                                            >  4:8228/1‑149 (MQ=255)
                     ccGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATg                                                                                                                          <  3:257795/148‑1 (MQ=255)
                              ttCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGc                                                                                                                                  <  4:241733/131‑1 (MQ=255)
                              ttCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGc                                                                                                                                  >  3:241733/1‑131 (MQ=255)
                                             gAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCg                                                                                                 >  6:39064/1‑149 (MQ=255)
                                                cTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAg                                                                                              <  3:8228/149‑1 (MQ=255)
                                                   cAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGc                                                                                             <  3:255363/147‑1 (MQ=255)
                                                    aGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCAGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGt                                                                                           >  1:127501/1‑148 (MQ=255)
                                                      aTCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGAc                                                                                        >  1:137498/1‑149 (MQ=255)
                                                       tCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACCTAACGGGGAGTAGTAGCGTGAcc                                                                                       >  6:223902/1‑149 (MQ=255)
                                                         gAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCtt                                                                                     <  4:9962/149‑1 (MQ=255)
                                                          aGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCtt                                                                                     >  7:41335/1‑148 (MQ=255)
                                                            gAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGaa                                                                                  <  8:193947/149‑1 (MQ=255)
                                                                 gCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATc                                                                             <  1:308697/149‑1 (MQ=255)
                                                                  ccAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATcc                                                                            <  2:33703/149‑1 (MQ=255)
                                                                       cccTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCg                                                                        <  2:36043/148‑1 (MQ=255)
                                                                        ccTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGc                                                                      >  7:213968/1‑149 (MQ=255)
                                                                        ccTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGc                                                                      >  5:270597/1‑149 (MQ=255)
                                                                          tGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGaa                                                                                  <  6:5126/135‑1 (MQ=255)
                                                                          tGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGaa                                                                                  >  5:5126/1‑135 (MQ=255)
                                                                                ggtgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGa                                                                                   <  8:179066/128‑1 (MQ=255)
                                                                                ggtgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGa                                                                                   >  7:179066/1‑128 (MQ=255)
                                                                                 gtgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGggtggt                                                             >  7:182070/1‑149 (MQ=255)
                                                                                    aaGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGgtgt                                                           <  8:41335/148‑1 (MQ=255)
                                                                                     tggCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGgtgtgt                                                         <  6:270597/148‑1 (MQ=255)
                                                                                     aGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGgtgtgt                                                         <  8:213968/149‑1 (MQ=255)
                                                                                               gcgGGCTGGCCGGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGc                                               <  8:213212/149‑1 (MQ=255)
                                                                                                             aTGCGGCCATTGCAGAAGCCCGTCGACTGCAGCGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTATCGTGACCTTCAAGAATCCAGCGGCAGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTg                                  >  6:105050/1‑148 (MQ=255)
                                                                                                                cGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCAc                               <  8:249304/148‑1 (MQ=255)
                                                                                                                    cATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCgg                          >  3:31256/1‑149 (MQ=255)
                                                                                                                              gCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGTGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGAc                <  2:269070/149‑1 (MQ=255)
                                                                                                                                ccGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGt              <  5:39064/149‑1 (MQ=255)
                                                                                                                                       cgacAGGGGCAACTGCCCCTGCCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAggt       >  3:56913/4‑149 (MQ=255)
                                                                                                                                         acAGGGGCCACTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTTGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGAGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAggtgg     >  5:264843/2‑149 (MQ=255)
                                                                                                                                          cAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAggtg      <  2:310745/147‑1 (MQ=255)
                                                                                                                                          cAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAggtg      <  4:31256/147‑1 (MQ=255)
                                                                                                                                            ggggCAACTGCCCCTGCCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAGGTGGTGa  <  4:56913/149‑1 (MQ=255)
                                                                                                                                             gggCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCAc                               >  3:116316/1‑119 (MQ=255)
                                                                                                                                             gggCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCAc                               <  4:116316/119‑1 (MQ=255)
                                                                                                                                                  gTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGgtggtg                                                            >  3:217394/1‑85 (MQ=255)
                                                                                                                                                  gTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGgtggtg                                                            <  4:217394/85‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CCCCGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAGGTGGTGA  >  NC_002947/3435776‑3436064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑