Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	262,752	T→A	11.5%	L90Q (CTG→CAG)	PP_0211 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	262,752	0	T	A	11.5%	183.5 / 12.0	69	L90Q (CTG→CAG)	PP_0211	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/32);  new base A (4/4);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.61e-01

TGCCGTGCGGCGCGCCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGC  >  NC_002947/262604‑262894                                                                                                                                                     |                                                                                                                                                tGCCGTGCGGCGCGCCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCt                                                                                                                                                 <  7:24162/149‑1 (MQ=255) tGCCGTGCGCCGCGCCTGTCCGGAGGCATCAGCCTGGAGCGCGACGATGTGCGCGTGGGGCAACTCGAGTCGCAAGCGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGAGCTACCCTTGGGCTTACTAGCGAGACCt                                                                                                                                                 <  6:73452/149‑1 (MQ=255)        cggcgCGCCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑g                                                                                                                                          <  7:34418/148‑1 (MQ=255)          gcgcgcCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTCACTTGC‑TGACCAGGTCacgcaa                                                                                                                                       <  8:153583/149‑6 (MQ=255)              gcCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTg                                                                                                                                    >  4:83920/1‑148 (MQ=255)              gcCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGC‑TGACCAGGTCACGCAAGtaa                                                                                                                                   <  8:238673/149‑3 (MQ=255)               ccTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGaa                                                                                                                                  <  2:156786/149‑1 (MQ=255)               ccTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGC‑TGACCAGGTCACGCAAGtaag                                                                                                                                  <  2:309039/149‑4 (MQ=255)                cTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAg                                                                                                                                 >  3:225747/1‑149 (MQ=255)                     cGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGAt                                                                                                                            >  8:145529/1‑149 (MQ=255)                     cGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGAt                                                                                                                            <  3:46236/149‑1 (MQ=255)                      tgAGGGATCAGCATGCTGCGCGACTATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGAATTGCGTACAACTGGTGATCAGCGATGGGCATGAGCGAGGGATCTACCATTCGGCTTACTTGCGTCACCTGGTCA‑GAAAGTGAAgataga                                                                                                                            >  6:327517/2‑143 (MQ=255)                      ggAGGGATCAGCCTGGTGCGCGACTATGTGCGCGTGGTGCAAATCGAGTGGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGc                                                                                                                                <  5:327517/144‑1 (MQ=255)                      ggAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATc                                                                                                                           >  6:311697/1‑149 (MQ=255)                       gAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCt                                                                                                                               <  4:97417/144‑1 (MQ=255)                       gAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCt                                                                                                                               >  3:97417/1‑144 (MQ=255)                            aTCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCa                                                                                                                     <  3:83920/149‑1 (MQ=255)                               aGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGtt                                                                                                                  >  4:263650/1‑149 (MQ=255)                               aGCCTGGTGCGCGACGATGTGCGCGGGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGtt                                                                                                                  <  8:56949/149‑1 (MQ=255)                                 ccTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGc                                                                                                                >  2:309929/1‑149 (MQ=255)                                  cTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGGAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGcc                                                                                                               <  3:74245/149‑1 (MQ=255)                                   tGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGtt                                                                                                                  <  8:66626/145‑1 (MQ=255)                                   tGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGtt                                                                                                                  >  7:66626/1‑145 (MQ=255)                                   tGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCg                                                                                                              >  5:189601/1‑149 (MQ=255)                                      tGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGaa                                                                                                           <  6:189601/149‑1 (MQ=255)                                      tGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGaa                                                                                                           <  6:307307/149‑1 (MQ=255)                                         gcgACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAggg                                                                                                        <  1:156321/149‑1 (MQ=255)                                           gacgaTGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGt                                                                                                      >  4:163769/1‑149 (MQ=255)                                           gacgaTGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGt                                                                                                      <  4:279997/149‑1 (MQ=255)                                               aTGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAg                                                                                                  <  5:311697/149‑1 (MQ=255)                                                         ggtgCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGgac                                                                                         >  5:97482/1‑148 (MQ=255)                                                          gtgCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGgacga                                                                                       >  3:257240/1‑149 (MQ=255)                                                            gCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAg                                                                                                          >  7:276821/1‑128 (MQ=255)                                                            gCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAg                                                                                                          <  8:276821/128‑1 (MQ=255)                                                            gCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTGGCCGGAAGGGGTGCAGAAGCAGGACGccc                                                                                     >  2:111233/1‑149 (MQ=255)                                                             cAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGAcc                                                                                     <  6:313065/148‑1 (MQ=255)                                                                    aGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGcc                                                                             <  3:163769/149‑1 (MQ=255)                                                                      tCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAg                                                                                            <  4:213910/132‑1 (MQ=255)                                                                      tCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAg                                                                                            >  3:213910/1‑132 (MQ=255)                                                                        gCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAAc                                                                          >  6:257967/1‑148 (MQ=255)                                                                         cAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCGTCAGTTGCCGGAAGGGGTGCAGAAGCAGGCCGACCGCCTCGCCGACg                                                                         >  8:80396/1‑148 (MQ=255)                                                                                aTGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGTCGGAATGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGcc                                                                 >  3:217755/1‑149 (MQ=255)                                                                                  ggCGGACCACTGGCGTGCAGCGATGGGCATGAGCGCGGGAGCTACCCTTGGGCTTACTTGCGGGACCTGGGCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGa                                                               <  8:230375/149‑1 (MQ=255)                                                                                    cGTACTACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAgg                                                             >  7:12696/1‑149 (MQ=255)                                                                                         aaCTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCa                                                                            >  1:157953/1‑129 (MQ=255)                                                                                         aaCTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCa                                                                            <  2:157953/129‑1 (MQ=255)                                                                                              gtgtTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAggg                                                                                                        <  1:241606/96‑1 (MQ=255)                                                                                              gtgtTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAggg                                                                                                        >  2:241606/1‑96 (MQ=255)                                                                                              gtgtTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGc                                                    <  1:309929/148‑1 (MQ=255)                                                                                               tgtTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCGGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGctc                                                  <  7:145529/149‑1 (MQ=255)                                                                                                    aGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTgg                                             <  3:263650/149‑1 (MQ=255)                                                                                                     gCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGc                                            <  6:97482/149‑1 (MQ=255)                                                                                                                 aGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGCCCGAGGGTAACCTGCTCTTTGGCGACACCCCGact                                >  4:133167/1‑147 (MQ=255)                                                                                                                  gcgcGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAgt                                <  7:80396/148‑1 (MQ=255)                                                                                                                    gcgGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGtgtg                             <  4:225747/149‑1 (MQ=255)                                                                                                                      gggATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTggg                           <  8:12696/149‑1 (MQ=255)                                                                                                                          tCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTa                         >  4:52875/1‑147 (MQ=255)                                                                                                                               ccTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTgggagatcg                     >  5:51387/1‑140 (MQ=255)                                                                                                                               ccTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTgg                            <  6:51387/139‑1 (MQ=255)                                                                                                                                      ttcaCTTGC‑TGACCAGGTCACGCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTgg           >  5:7980/4‑149 (MQ=255)                                                                                                                                      ttcaCTTGC‑TGACCAGGTCACGCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTgg           >  7:185927/4‑149 (MQ=255)                                                                                                                                       tcaCTTGC‑TGACCAGGTCACGCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGTgg                                                             >  1:266393/3‑98 (MQ=38)                                                                                                                                       tcaCTTGC‑TGACCAGGTCACGCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAgg                                                             <  2:266393/96‑1 (MQ=38)                                                                                                                                         aCTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGc         >  7:29220/1‑148 (MQ=255)                                                                                                                                           ttGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAg                                                                                                          >  5:45081/1‑49 (MQ=255)                                                                                                                                           ttGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAg                                                                                                          <  6:45081/49‑1 (MQ=255)                                                                                                                                             gCGTGACCTGGTCA‑TCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAAc      <  4:257240/147‑1 (MQ=255)                                                                                                                                             gCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAAc      <  3:52875/147‑1 (MQ=255)                                                                                                                                              gctGACCAGGTCACGCAAGT‑AAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGCCGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTg   >  6:274301/3‑149 (MQ=255)                                                                                                                                              cGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGCCAACGTg   >  5:321202/1‑149 (MQ=255)                                                                                                                                               gTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACAGGCCGAGGCTAACCTGCTCTTTGGCGACACCCAGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGc  >  7:297314/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                TGCCGTGCGGCGCGCCTGTCCGGAGGGATCAGCCTGGTGCGCGACGATGTGCGCGTGGTGCAAATCGAGTCGCAAGGGTATGGCGTACAACTGGTGTTCAGCGATGGGCATGAGCGCGGGATCTACCCTTGGGCTTACTTGCGTGACCTGGTCA‑GCAAGTGAAGCTGATCCCTTCAGTTGCCGGAAGGGGTGCAGAAGCAGGACGACCGCCTAGCCAACGAACTGGCCGAGGGTAACCTGCTCTTTGGCGACACCCCGAGTGTGGGTACAGGGCGCAACTGGGCAACGTGC  >  NC_002947/262604‑262894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑