Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,466,841 T→G 16.6% intergenic (‑16/+108) PP_3081 ← / ← PP_5560 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,466,8410TG16.6% 76.4 / 8.9 47intergenic (‑16/+108)PP_3081/PP_5560hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (9/27);  new base G (6/2);  total (18/29)
Fisher's exact test for biased strand distribution p-value = 1.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

TGCGGGCAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTC  >  NC_002947/3466700‑3466980
                                                                                                                                             |                                                                                                                                            
tGCGGGCAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGcggcgg                                                                                                                                       >  2:81970/1‑149 (MQ=255)
   gggCAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCT‑GCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCGCAGGCGGCCaa                                                                                                                                   <  3:221117/149‑1 (MQ=255)
      cAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACg                                                                                                                                 <  5:134782/149‑1 (MQ=255)
          ttGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCtgt                                                                                                                             >  6:139611/1‑149 (MQ=255)
              ccccGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTGGCAGGGCAATGACGCCGCCATCAGTGAAAGCCGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGGG‑CAg                                                                                                                        <  3:203542/149‑1 (MQ=255)
                 aGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCAGCGGCGGCCAACGCTGTG‑GACGgc                                                                                                                     >  7:60450/1‑149 (MQ=255)
                  gCTCAGTTGCGGGGTCCGCTCTTCACCGGAACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGTAACTGACCTAAATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGgca                                                                                                                    <  6:205232/149‑1 (MQ=255)
                   cTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGgcag                                                                                                                   <  8:6180/149‑1 (MQ=255)
                      aGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGCAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGgcagca                                                                                                                 <  4:51768/148‑1 (MQ=255)
                        ttGGGGGGTCCGCTCTTCACCGGCACCGGATGCCGGGTCCTGGCCTACGTGTTGCAGGGAAATTACGCCGCCAACTTTGAAAGACGAGTAATTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAAAGCTGTG‑CAGGGCAGCAAag                                                                                                              <  2:260755/149‑1 (MQ=255)
                         tGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCCCCTGGGGCGGGCAACGCTGTG‑GAGGGCCCCaaacc                                                                                                             >  3:147090/1‑147 (MQ=255)
                          gCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAagc                                                                                                             <  5:139611/148‑1 (MQ=255)
                                tCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAAc                                                                                                      <  4:110570/149‑1 (MQ=255)
                                  cGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGCACAg                                                                                                    >  8:194799/1‑149 (MQ=255)
                                             ggCACCGCATGCCGGTTCCTGGCCCACCTGTTGCAGGCCAATGACGCCGCCATCATTTAAAGACTAGTAAGTCACCTACATCGCCCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGa                                                                                         <  4:147090/149‑1 (MQ=255)
                                             ggCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGa                                                                                         <  1:81970/149‑1 (MQ=255)
                                                 tgcggtGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTGCCAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGt                                                                                      <  1:53394/144‑1 (MQ=255)
                                                            gTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGCCGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATc                                                                          <  7:287351/149‑1 (MQ=255)
                                                               cTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGGCCACGCGGTG‑CCGGGCAGCACACCACCGAACACCCAGAGTCGGTGGTGCATGGTGAATACtt                                                                       >  8:123683/1‑149 (MQ=255)
                                                                  gCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAg                                                                                                >  5:322340/1‑121 (MQ=255)
                                                                  gCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAg                                                                                                <  6:322340/121‑1 (MQ=255)
                                                                   ccGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCa                                                                   <  4:59948/149‑1 (MQ=255)
                                                                          gTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCCCCAGCGGCGGCCNACCCGGTG‑CAGGGGAGCAAAGCAGCGGACAGCCCGTGGGGGAGGTGGAAGGTAAATGCTTGGGAGGGCGt                                                             >  1:104454/1‑148 (MQ=255)
                                                                           ttGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGGCGCCGGCGGGGGCCCCCGCCGTG‑CCGGGCCGGCAAGAAGCGCACCCCCAGTGTGTGGGGTGGATGGTGAATGCGTGGGAggggggga                                                           >  2:308842/1‑144 (MQ=255)
                                                                             gCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGGGGCGGCCAAAGCTGTG‑CACGGCAGCAAAGCAGCGCACAGCCAGGGGCGGAGGTGCAAGGTCAATCCATGGCAGGGCGTCAcc                                                         >  7:193921/1‑148 (MQ=255)
                                                                              cAGGGCAATGACGCGGCCATCAGTCAACCAGGAGGACCGGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCATAGCAGCGAACAGCCAGTGTCTGAGGTGCTTGGTCAATCCTTGGCAGGGCGTCACgg                                                        <  7:222524/149‑1 (MQ=255)
                                                                                gggCAATGACGCCGCCATCAGTGAAACACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGGCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCt                                                      <  8:90852/149‑1 (MQ=255)
                                                                                  gCAATGACGCCGCCATCAGTGAATGACGAGGAACTCACCGACATCGCGCCTGGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCa                                                    <  5:198908/149‑1 (MQ=255)
                                                                                            cgccATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCGGCGGCGGCCAACCCAGTG‑CAGGGCCGCAAAGCAGCGAACAGCCAGTGTGTGAGGTGCATGGTCAATCCATGGCAGGGCGGGACGGCGCAGTCTAGCCCa                                          >  6:7012/1‑149 (MQ=255)
                                                                                              ccATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAg                                         <  1:170806/148‑1 (MQ=255)
                                                                                               cATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAggg                                                                <  7:273369/124‑1 (MQ=255)
                                                                                                    gTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCCCCAGCGGCGGCCCACGCGGTG‑GAGGGCAGCAAAGCCGCGCACAGCCAGGGTCTGCGGTGGATGGGCAAACCTTGGCCGGGGGTCCCGGCTGCGGGTAGCCCCGGTGTGca                                  >  6:62245/1‑148 (MQ=255)
                                                                                                         aGACGAGGAACTGACCGACATCGCGCCTTGGCGGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAACGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCTTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGc                             <  1:209075/149‑1 (MQ=255)
                                                                                                                  aCTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAg                                                   >  7:150066/1‑118 (MQ=255)
                                                                                                                  aCTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAg                                                   <  8:150066/118‑1 (MQ=255)
                                                                                                                  aCTGACCGACATCGCGCCTTGGCCGCCGGCGGCGGCCAACCCTGTG‑CATGGCAGCAAAGCACCGAACAGCCCGTGTCTGTGGTGCATGGTGAATCCGTGGCAGGGCGTCACGGGGCAGTCTACCCCAGGGGAGGTTGACCGGCTGTccg                    >  2:30786/1‑148 (MQ=255)
                                                                                                                      aCCGACATCCCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTc                <  2:104454/149‑1 (MQ=255)
                                                                                                                        cGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCt              <  4:307360/149‑1 (MQ=255)
                                                                                                                           cATCGGGCCTTGGCCGCGTGGGGCGGCCCACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGGCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCgg           <  5:188926/149‑1 (MQ=255)
                                                                                                                               gcgcGTTGGCCGCCTGCGGGGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGGCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAg        <  7:123683/148‑1 (MQ=255)
                                                                                                                                 gcgttGGCCGCCGCAGGCGGCCAAGGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGc     >  8:13537/4‑149 (MQ=255)
                                                                                                                                  ccTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGt       >  3:69403/1‑146 (MQ=255)
                                                                                                                                  ccTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGt       <  4:69403/146‑1 (MQ=255)
                                                                                                                                    ttGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTc  <  8:193921/149‑1 (MQ=255)
                                                                                                                                    ttGGCCGCCGCAGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTc  >  3:315733/1‑149 (MQ=255)
                                                                                                                                    ttGGCCGCCGCAGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTc  >  8:3434/1‑149 (MQ=255)
                                                                                                                                      ggCCGCCGGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTc  <  7:201061/147‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
TGCGGGCAGGTTGTCCCAGCTCAGTTGCGGGGTCCGCTCTTCACCGGCACCGCATGCCGGGTCCTGGCCGACCTGTTGCAGGGCAATGACGCCGCCATCAGTGAAAGACGAGGAACTGACCGACATCGCGCCTTGGCCGCCTGCGGCGGCCAACGCTGTG‑CAGGGCAGCAAAGCAGCGAACAGCCAGTGTCTGAGGTGCATGGTCAATCCTTGGCAGGGCGTCACGGCTCAGTCTAGCCCAGGTCTGCTTCAGCGGCTGTCCTGGTCCTCGGCAGTGCCTC  >  NC_002947/3466700‑3466980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑