Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,474,290 T→C 10.0% D808G (GAT→GGT)  PP_3087 ← excinuclease ABC subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,474,2900TC10.0% 101.8 / 2.7 40D808G (GAT→GGT) PP_3087excinuclease ABC subunit A
Reads supporting (aligned to +/- strand):  ref base T (16/20);  new base C (2/2);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

GATCGCATTCTAATCGGCGGCCCAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCAGGCGGTTCAGTTGTACCAGCAGCCGGTCGATGTCCTGCGGGTG  >  NC_002947/3474150‑3474429
                                                                                                                                             |                                                                                                                                           
gATCGCATTCTAATCGGCGGCCCAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGcc                                                                                                                                     >  6:82197/1‑149 (MQ=255)
gATCGCATTCTAATCGGCGGCCCAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGc                                                                                                                                      <  6:102204/148‑1 (MQ=255)
  tCGCATTCTAATCGGCGGCCCAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCACCACCAGcccc                                                                                                                                   >  3:73635/1‑149 (MQ=255)
  tCGCATTCTAATCGGCGGCCCAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCACCACCAGcccc                                                                                                                                   >  3:69256/1‑149 (MQ=255)
    gCATTCTAATCGGCGGCCCAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCGCCCGGCATCACCAGCCCCCg                                                                                                                                 >  1:25534/1‑149 (MQ=255)
    gCATTCTAATCGGCGGCCCAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCg                                                                                                                                 >  6:149219/1‑149 (MQ=255)
      aTTCTAATCGGCGGCCCAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGc                                                                                                                               >  6:211595/1‑149 (MQ=255)
        tCTAATCGGCGGCCCAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTCACCACCAC‑CTTCCCCCCCGCATACCCAGCCCCCGGccc                                                                                                                             >  2:221776/1‑149 (MQ=255)
             tCGGCGGCCCAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGatat                                                                                                                        >  3:270949/1‑149 (MQ=255)
             atatcggcCCAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGatat                                                                                                                        <  7:277752/145‑1 (MQ=255)
                gcggcCCATGAAACTGGGAATGCGGAGGGGTTTCAATGGCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTTCATGCCGCCACCACCTTCGGCGTGCCACTGACCACCACTCTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCg                                                                                                                      <  1:221776/149‑1 (MQ=255)
                    cccAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCACCACCAGCCCCCGGCCCGATATCGATCAc                                                                                                                 <  4:73635/149‑1 (MQ=255)
                    cccAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCACCACCAGCCCCCGGCCCGATATCGATCAc                                                                                                                 <  4:69256/149‑1 (MQ=255)
                      cAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCAccc                                                                                                               <  2:25534/149‑1 (MQ=255)
                      cAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCAcc                                                                                                                >  4:37138/1‑148 (MQ=255)
                              tGGCAATGCGCAGGGGTTACAAGGCCATTGCCAAAAACGTCGCCGTGCGGCTGTCGCTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTAGCCCACGCCATCACCAGCCCCCCCCACGATATAGATCACCCACTCGctc                                                                                                       >  6:200849/1‑149 (MQ=255)
                                 cAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTgc                                                                                                    >  4:134523/1‑149 (MQ=255)
                                             gTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCa                                                                                                                                           <  5:295344/98‑1 (MQ=255)
                                             gTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCa                                                                                                                                           >  6:295344/1‑98 (MQ=255)
                                                  aaGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTc                                                                                   <  3:313511/149‑1 (MQ=255)
                                                  aaGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTc                                                                                   >  6:237425/1‑149 (MQ=255)
                                                  aaGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATAACCAGCCCCCGGCCCGATATCGATCACCCATTCGCTCTGCGCCACCACTAGCATGTc                                                                                   >  3:63926/1‑149 (MQ=255)
                                                          tGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTc                                                                             <  5:82197/147‑1 (MQ=255)
                                                            ccAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGac                                                                          <  3:37138/148‑1 (MQ=255)
                                                                  aCGTCGCCGTGCGGCTGTCGGTACATGCCGCCACAACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCTCCACCACACGCATGTCGTTTTAGaccaccacc                                                                   >  4:226462/1‑149 (MQ=255)
                                                                                   tCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGTGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCttt                                                   <  5:211595/148‑1 (MQ=255)
                                                                                   gcGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGAACACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTc                                                  <  7:83875/148‑1 (MQ=255)
                                                                                         cATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCa                                             <  2:122655/148‑1 (MQ=255)
                                                                                             cagccACCACCTGCTGCTTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCACGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCAGGCgg                                        <  6:323283/147‑1 (MQ=255)
                                                                                                    caccTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCAGGCGGTTCAGtt                                 <  1:37413/149‑1 (MQ=255)
                                                                                                      ccTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCAGGCGGTTCAGTTGt                               <  5:200849/149‑1 (MQ=255)
                                                                                                           ggcgTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCAGGCGGTTCAGTTGTACcag                          <  2:86938/149‑1 (MQ=255)
                                                                                                               tGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCAGGCGGTTCAGTTGTACCagcagc                      <  4:270949/149‑1 (MQ=255)
                                                                                                                 ccACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTAGACCACCACCACGCTATGCCCGCGTACTACCAGGCGGTTCAGTTGTACCAGCAGCCg                    >  1:35738/1‑149 (MQ=255)
                                                                                                                          accac‑cTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCAGGCGGTTCAGTTGTACCAGCAGCCGGTCGATGt            >  7:305038/1‑148 (MQ=255)
                                                                                                                          accac‑cTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCAGGCGGTTCAGTTGTACCAGCAGCCGGTCGATGTc           <  8:305038/149‑1 (MQ=255)
                                                                                                                              c‑cTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCAGGCGGTTCAGTTGTACCAGCAGCCGGTCGATGTCCTGc       <  3:134523/149‑1 (MQ=255)
                                                                                                                                   gCCCCCGTCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCTCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCAGGCGGTTCAGTTGTACCAGCAGCCGGTCGATGTCCTGCggg    <  3:226462/148‑1 (MQ=255)
                                                                                                                                   gCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCAGGCGGTTCAGTTGTACCAGCAGCCGGTCGATGTCCTGCgg     <  1:245837/147‑1 (MQ=255)
                                                                                                                                     ccccGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTATCTACCAGGCGGTTCAGTTGTACCAGCAGCCGGTCGATGTCCTGCGGGTg  >  7:149419/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                           
GATCGCATTCTAATCGGCGGCCCAGGAAACTGGCAATGCGCAGGGGTTACAAGGCCTTTGCCAAAAACGTCGCCGTGCGGCTGTCGGTACATGCCGCCACCACCTGCGGCGTGCCACTGACCACCAC‑CTTGCCCCCGGCATCACCAGCCCCCGGCCCGATATCGATCACCCAGTCGCTCTGCGCCACCACTCGCATGTCGTGTTCGACCACCACCACGCTATGCCCGCTTTCTACCAGGCGGTTCAGTTGTACCAGCAGCCGGTCGATGTCCTGCGGGTG  >  NC_002947/3474150‑3474429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑