Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,577,653 T→A 10.4% L23Q (CTG→CAG)  PP_3158 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,577,6530TA10.4% 133.9 / 5.9 48L23Q (CTG→CAG) PP_3158hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/22);  new base A (3/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

CCGCACTTTTTCCCATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCTGTACCTGCTGATG  >  NC_002947/3577507‑3577795
                                                                                                                                                  |                                                                                                                                              
ccGCACTTTTTCCCATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCtgt                                                                                                                                              <  5:264192/149‑1 (MQ=255)
         tttccATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCGCGGCGAAGACGCCCAATATCGACCCGGTGCCTATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTTGCATGCCTGTGCGCCttt                                                                                                                                      <  8:282365/145‑3 (MQ=255)
           cccATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTAc                                                                                                                                    <  7:255357/148‑1 (MQ=255)
           cccATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGCGCACAGGCATGCCAGGGCACCTGtcag                                                                                                                                   >  5:242876/1‑146 (MQ=255)
             cATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGCGCACAGGCATGCCAGGGCACCTGtcagta                                                                                                                                 <  6:242876/149‑6 (MQ=38)
                      gCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAggg                                                                                                                        <  4:260706/149‑1 (MQ=255)
                          tATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGt                                                                                                                    >  5:36810/1‑149 (MQ=255)
                                 ggCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGATGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATg                                                                                                              <  3:149358/148‑1 (MQ=255)
                                   ccAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTc                                                                                                           <  1:21422/149‑1 (MQ=255)
                                    cAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTc                                                                                                           >  2:315192/1‑148 (MQ=255)
                                     aGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCaa                                                                                                         <  1:144149/149‑1 (MQ=255)
                                       gCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAAcc                                                                                                       <  3:229549/149‑1 (MQ=255)
                                                 cTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTcggcaa                                                                                             <  2:73812/149‑1 (MQ=255)
                                                    ccGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTcggcaacgg                                                                                          >  4:229969/1‑149 (MQ=255)
                                                    ccGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTCCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACCCAGCAGCAGGGCCGTGACATGTTCAACCAGCTaggcaacgg                                                                                          >  2:292033/1‑149 (MQ=255)
                                                            gccgccCAATCTCCACCCGGTGCCGACGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGGGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCa                                                                                  <  8:210730/149‑1 (MQ=255)
                                                                    atatCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCGGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTg                                                                          <  8:49228/149‑1 (MQ=255)
                                                                     tatCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGt                                                                         <  6:36810/149‑1 (MQ=255)
                                                                       tCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTc                                                                       >  1:42693/1‑149 (MQ=255)
                                                                          aCCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGAAATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAg                                                                    <  3:23064/149‑1 (MQ=255)
                                                                           cccGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGa                                                                   <  3:310197/149‑1 (MQ=255)
                                                                           cccGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGa                                                                   >  7:147993/1‑149 (MQ=255)
                                                                            ccGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGAc                                                                  >  5:96773/1‑149 (MQ=255)
                                                                              ggTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCa                                                                <  8:147993/149‑1 (MQ=255)
                                                                                tGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGc                                                              >  3:103177/1‑149 (MQ=255)
                                                                                       ggTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGAc                                                        <  4:103177/148‑1 (MQ=255)
                                                                                                    gcgAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTc                                                                       <  6:119135/120‑1 (MQ=255)
                                                                                                    gcgAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTc                                                                       >  5:119135/1‑120 (MQ=255)
                                                                                                    gcgAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCt                                          >  4:254124/1‑149 (MQ=255)
                                                                                                    gcgAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCt                                          >  5:134129/1‑149 (MQ=255)
                                                                                                      gAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGt                                        <  2:42693/149‑1 (MQ=255)
                                                                                                          ttCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGAt                                    >  3:260968/1‑149 (MQ=255)
                                                                                                                         cTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGc                      <  6:96773/148‑1 (MQ=255)
                                                                                                                          tACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCATTCATACGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCa                     >  1:272879/1‑148 (MQ=255)
                                                                                                                          tACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGCCAACCTGCa                     >  8:246045/1‑148 (MQ=255)
                                                                                                                            cgtaCAGGCGCACAGGCATGCCAGGGCACCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAg                  >  7:304677/4‑149 (MQ=255)
                                                                                                                               aCAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACGTGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGG‑CACGCTGTCGATGCGCGACAACCTGCAAAGcc                <  1:311263/147‑1 (MQ=255)
                                                                                                                               aCAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCt               >  1:95974/1‑149 (MQ=255)
                                                                                                                                 aGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGCCCAGCGGCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCTg              >  3:38513/1‑148 (MQ=255)
                                                                                                                                  ggTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGAc                                                        >  7:175305/1‑105 (MQ=255)
                                                                                                                                  ggTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGAc                                                        <  8:175305/105‑1 (MQ=255)
                                                                                                                                   gTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCTGTAc           >  7:150891/1‑149 (MQ=255)
                                                                                                                                     gcacaggCATGCCAGGGCACCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTg                       <  1:316752/130‑1 (MQ=255)
                                                                                                                                     gcacaggCATGCCAGGGCACCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTg                       >  2:316752/6‑135 (MQ=255)
                                                                                                                                     gCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCTGTACct         >  1:34809/1‑149 (MQ=255)
                                                                                                                                       ccTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCt               <  3:167293/141‑1 (MQ=255)
                                                                                                                                       ccTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCt               >  4:167293/1‑141 (MQ=255)
                                                                                                                                           gCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCTGTACCTACtga    <  6:134129/148‑1 (MQ=255)
                                                                                                                                             aTGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACCCTGTCGATGCGCGACAACCTGCAAAGCCTGTACCTGCtgaag  >  1:40335/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CCGCACTTTTTCCCATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCTGTACCTGCTGATG  >  NC_002947/3577507‑3577795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑