Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,577,658 G→A 10.1% A25T (GCC→ACC)  PP_3158 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,577,6580GA10.1% 133.5 / 4.0 49A25T (GCC→ACC) PP_3158hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (21/23);  new base A (3/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCCATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCTGTACCTGCTGATGAACAAGC  >  NC_002947/3577516‑3577802
                                                                                                                                              |                                                                                                                                                
tttccATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCGCGGCGAAGACGCCCAATATCGACCCGGTGCCTATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTTGCATGCCTGTGCGCCttt                                                                                                                                             <  8:282365/145‑3 (MQ=255)
  cccATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTAc                                                                                                                                           <  7:255357/148‑1 (MQ=255)
  cccATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGCGCACAGGCATGCCAGGGCACCTGtcag                                                                                                                                          >  5:242876/1‑146 (MQ=255)
    cATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGCGCACAGGCATGCCAGGGCACCTGtcagta                                                                                                                                        <  6:242876/149‑6 (MQ=38)
             gCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAggg                                                                                                                               <  4:260706/149‑1 (MQ=255)
                 tATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGt                                                                                                                           >  5:36810/1‑149 (MQ=255)
                        ggCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGATGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATg                                                                                                                     <  3:149358/148‑1 (MQ=255)
                          ccAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTc                                                                                                                  <  1:21422/149‑1 (MQ=255)
                           cAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTc                                                                                                                  >  2:315192/1‑148 (MQ=255)
                            aGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCaa                                                                                                                <  1:144149/149‑1 (MQ=255)
                              gCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAAcc                                                                                                              <  3:229549/149‑1 (MQ=255)
                                        cTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTcggcaa                                                                                                    <  2:73812/149‑1 (MQ=255)
                                           ccGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTcggcaacgg                                                                                                 >  4:229969/1‑149 (MQ=255)
                                           ccGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTCCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACCCAGCAGCAGGGCCGTGACATGTTCAACCAGCTaggcaacgg                                                                                                 >  2:292033/1‑149 (MQ=255)
                                                   gccgccCAATCTCCACCCGGTGCCGACGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGGGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCa                                                                                         <  8:210730/149‑1 (MQ=255)
                                                           atatCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCGGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTg                                                                                 <  8:49228/149‑1 (MQ=255)
                                                            tatCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGt                                                                                <  6:36810/149‑1 (MQ=255)
                                                              tCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTc                                                                              >  1:42693/1‑149 (MQ=255)
                                                                 aCCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGAAATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAg                                                                           <  3:23064/149‑1 (MQ=255)
                                                                  cccGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGa                                                                          >  7:147993/1‑149 (MQ=255)
                                                                  cccGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGa                                                                          <  3:310197/149‑1 (MQ=255)
                                                                   ccGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGAc                                                                         >  5:96773/1‑149 (MQ=255)
                                                                     ggTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCa                                                                       <  8:147993/149‑1 (MQ=255)
                                                                       tGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGc                                                                     >  3:103177/1‑149 (MQ=255)
                                                                              ggTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGAc                                                               <  4:103177/148‑1 (MQ=255)
                                                                                           gcgAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTc                                                                              >  5:119135/1‑120 (MQ=255)
                                                                                           gcgAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTc                                                                              <  6:119135/120‑1 (MQ=255)
                                                                                           gcgAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCt                                                 >  4:254124/1‑149 (MQ=255)
                                                                                           gcgAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCt                                                 >  5:134129/1‑149 (MQ=255)
                                                                                             gAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGt                                               <  2:42693/149‑1 (MQ=255)
                                                                                                 ttCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGAt                                           >  3:260968/1‑149 (MQ=255)
                                                                                                                cTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGc                             <  6:96773/148‑1 (MQ=255)
                                                                                                                 tACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCATTCATACGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCa                            >  1:272879/1‑148 (MQ=255)
                                                                                                                 tACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGCCAACCTGCa                            >  8:246045/1‑148 (MQ=255)
                                                                                                                   cgtaCAGGCGCACAGGCATGCCAGGGCACCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAg                         >  7:304677/4‑149 (MQ=255)
                                                                                                                      aCAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACGTGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGG‑CACGCTGTCGATGCGCGACAACCTGCAAAGcc                       <  1:311263/147‑1 (MQ=255)
                                                                                                                      aCAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCt                      >  1:95974/1‑149 (MQ=255)
                                                                                                                        aGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGCCCAGCGGCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCTg                     >  3:38513/1‑148 (MQ=255)
                                                                                                                         ggTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGAc                                                               >  7:175305/1‑105 (MQ=255)
                                                                                                                         ggTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGAc                                                               <  8:175305/105‑1 (MQ=255)
                                                                                                                          gTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCTGTAc                  >  7:150891/1‑149 (MQ=255)
                                                                                                                            gcacaggCATGCCAGGGCACCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTg                              >  2:316752/6‑135 (MQ=255)
                                                                                                                            gcacaggCATGCCAGGGCACCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTg                              <  1:316752/130‑1 (MQ=255)
                                                                                                                            gCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCTGTACct                >  1:34809/1‑149 (MQ=255)
                                                                                                                              ccTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCt                      >  4:167293/1‑141 (MQ=255)
                                                                                                                              ccTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCt                      <  3:167293/141‑1 (MQ=255)
                                                                                                                                  gCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCTGTACCTACtga           <  6:134129/148‑1 (MQ=255)
                                                                                                                                    aTGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACCCTGTCGATGCGCGACAACCTGCAAAGCCTGTACCTGCtgaag         >  1:40335/1‑146 (MQ=255)
                                                                                                                                           tgCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCTGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCGAAGCCTGTACCTGCTGATGAACAAGc  <  1:315192/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
TTCCCATTTTCGGGCTCTATGCTTGGCCAGGCCGCGCTGACTCCCGGCGCAGCCGCCCAATATCGACCCGGTGCCGATGGTTGCAAGCTACGCGAATTTCCTCCCCCGCCTGCTACTGACAGGTGCCCTGGCATGCCTGTGCGCCTGTACGCAGCAGCAGGGCCGTGACATGGTCAACCAGCTCGGCAACGGCAAACCCAGCGAGCTGTTCCAGACCAGCGTCGACCGCCTGGCCACGCTGTCGATGCGCGACAACCTGCAAAGCCTGTACCTGCTGATGAACAAGC  >  NC_002947/3577516‑3577802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑