Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,584,899 A→C 7.6% intergenic (+46/‑89) benC → / → benD benzoate 1,2‑dioxygenase electron transfer component/1,6‑dihydroxycyclohexa‑2,4‑diene‑1‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,584,8990AC 7.6% 148.2 / 2.6 50intergenic (+46/‑89)benC/benDbenzoate 1,2‑dioxygenase electron transfer component/1,6‑dihydroxycyclohexa‑2,4‑diene‑1‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (24/22);  new base C (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCCGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGCCCAA  >  NC_002947/3584755‑3585038
                                                                                                                                                |                                                                                                                                           
ggCCCGCCACGCATGGTGGAAGGGGTCAGCCTGTACGTGCGTGAGCAGTGAATTACCCGTGCCAACTTCTACTAGGTGAAGTTTGCGGCTGCGGCCTGAGGCTCCCTGATTCATAGCAATTCGGCGAGCGTGTTGGAGCGGGCGAGacc                                                                                                                                         <  2:26231/149‑1 (MQ=255)
 gCCCGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCg                                                                                                                                        >  1:183796/1‑149 (MQ=255)
   ccGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCt                                                                                                                                      <  1:179753/149‑1 (MQ=255)
     gCCACCCATGGTGGAAGCGGTCAGACAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCTCGCCCGCTcc                                                                                                                                    <  4:281111/149‑1 (MQ=255)
                  gAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATcgcg                                                                                                                       >  1:228056/1‑149 (MQ=255)
                     gCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAg                                                                                                                    <  2:156116/149‑1 (MQ=255)
                       ggTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGcc                                                                                                                  >  3:207121/1‑149 (MQ=255)
                         tCAGCCAGTACGTGCGTGAGCAGAGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCTCGCCCGCTc                                                                                                                                     >  7:281612/1‑128 (MQ=255)
                         tCAGCCAGTACGTGCGTGAGCAGAGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCTCGCCCGCTc                                                                                                                                     <  8:281612/128‑1 (MQ=255)
                            gCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCa                                                                                                             <  2:183796/149‑1 (MQ=255)
                             ccAGTGCGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAg                                                                                                            >  7:292304/1‑149 (MQ=255)
                                   cgtgcgtcAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGAGCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCttt                                                                                                       <  5:52344/148‑1 (MQ=255)
                                    gtgcgtgAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGa                                                                                                     <  3:20605/149‑1 (MQ=255)
                                    gtgcgtgAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGa                                                                                                     <  5:22012/149‑1 (MQ=255)
                                       cgtgAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGc                                                                                                   <  4:207121/148‑1 (MQ=255)
                                       cgtgAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGACTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGc                                                                                                   >  4:3747/1‑148 (MQ=255)
                                                      aCCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCca                                                                                   >  3:156500/1‑149 (MQ=255)
                                                        cccGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCca                                                                                   >  4:77090/1‑147 (MQ=255)
                                                            gCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGt                                                                             <  4:156500/149‑1 (MQ=255)
                                                                     tactacGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTcgcg                                                                     >  2:114414/1‑148 (MQ=255)
                                                                       ctacGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGaa                                                                   <  6:128900/148‑1 (MQ=255)
                                                                          cGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACt                                                                 <  8:292304/147‑1 (MQ=255)
                                                                           gagaAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCaa                                                                                                 <  5:137681/114‑1 (MQ=255)
                                                                           gagaAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCaa                                                                                                 >  6:137681/1‑114 (MQ=255)
                                                                           gagaAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTg                                                                >  6:285530/1‑147 (MQ=255)
                                                                                 tttGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGAttt                                                         >  3:297920/1‑148 (MQ=255)
                                                                                   tgcggctgcggcCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTAc                                                       <  7:25807/148‑1 (MQ=255)
                                                                                      ggctgcggcCTGACGCTCCCTGATTCAAAGCAATACGCCGACGCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACatat                                                   <  1:114414/149‑1 (MQ=255)
                                                                                         tgcggcCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCGGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACat                                                     >  4:207011/1‑144 (MQ=255)
                                                                                         tgcggcCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCGGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACat                                                     <  3:207011/144‑1 (MQ=255)
                                                                                         tgcggcCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGaa                                                <  5:285530/149‑1 (MQ=255)
                                                                                              ccTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGaacaa                                             >  5:237552/1‑147 (MQ=255)
                                                                                               cTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGaacaa                                             >  2:306548/1‑146 (MQ=255)
                                                                                                 gACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGAt                                        >  7:121301/1‑149 (MQ=255)
                                                                                                  aCGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGa                                         <  2:228056/147‑1 (MQ=255)
                                                                                                    gCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTc                                      >  3:204623/1‑148 (MQ=255)
                                                                                                                aaaGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGAtt                                       >  5:266697/1‑135 (MQ=255)
                                                                                                                aaaGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGAtt                                       <  6:266697/135‑1 (MQ=255)
                                                                                                                aaaGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTg                         >  7:92594/1‑149 (MQ=255)
                                                                                                                aaaGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTg                         >  4:270170/1‑149 (MQ=255)
                                                                                                                aaaGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCGCCAACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGATGCTGCCACAGGGTCTCTCGAGAAAGGGAGAGTTACATATGAACATCAGATTCGAAGGAAAGTTTg                         >  5:248024/1‑149 (MQ=255)
                                                                                                                 aaGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGc                        >  4:224488/1‑149 (MQ=255)
                                                                                                                  aGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGt                                                                             >  1:317402/1‑95 (MQ=255)
                                                                                                                  aGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGt                                                                             <  2:317402/95‑1 (MQ=255)
                                                                                                                   gCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGccc                      <  8:92594/149‑1 (MQ=255)
                                                                                                                        aCGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGAttt                                                         <  4:105556/109‑1 (MQ=255)
                                                                                                                        aCGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGAttt                                                         >  3:105556/1‑109 (MQ=255)
                                                                                                                                ccTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTgc         >  1:20263/1‑149 (MQ=255)
                                                                                                                                       gAGCGGGCTCGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAGCAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGccc    >  1:156374/1‑147 (MQ=255)
                                                                                                                                       gAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGCCCaa  <  3:3747/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
GGCCCGCCACCCATGGTGGAAGCGGTCAGCCAGTACGTGCGTGAGCAGGGCATTACCCCGGCCAACTTCTACTACGAGAAGTTTGCGGCTGCGGCCTGACGCTCCCTGATTCAAAGCAATACGCCGACCCTGTGGGAGCGGGCGAGCCCGCTCCCACAGTGATCGCGCAGCCTTTCAGGCTTTGAGCAAGAGAGTTGCTGCCACAGGGTCTCTCGCGAACTGGAGATTTACATATGAACAACAGATTCCAAGGCAAGGTTGCCCTGGTCACCGGTGCCGCCCAA  >  NC_002947/3584755‑3585038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑