Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,644,948 C→T 14.8% intergenic (‑43/+23) PP_3211 ← / ← PP_3212 ABC transporter ATP‑binding protein/Rieske 2Fe‑2S family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,644,9480CT14.8% 109.3 / 9.0 47intergenic (‑43/+23)PP_3211/PP_3212ABC transporter ATP‑binding protein/Rieske 2Fe‑2S family protein
Reads supporting (aligned to +/- strand):  ref base C (21/19);  new base T (3/4);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

TTTTTTCCACCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTGCGGCGGGTGTCGACAC  >  NC_002947/3644806‑3645095
                                                                                                                                              |                                                                                                                                                   
ttttttCCACCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCg                                                                                                                                               <  1:172871/149‑1 (MQ=255)
   tttCCACCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGTGCGCCggg                                                                                                                                             <  5:181895/148‑1 (MQ=255)
    ttCCACCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCgggg                                                                                                                                            <  4:223663/148‑1 (MQ=255)
      ccACCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGt                                                                                                                                         >  5:290212/1‑149 (MQ=255)
      ccACCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTACGCCCCGGTGCGCCGGGGca                                                                                                                                          <  8:119063/148‑2 (MQ=255)
       cACCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGtt                                                                                                                                        <  5:274846/149‑1 (MQ=255)
        aCCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGTGCGCCGGGGcatac                                                                                                                                       <  7:116303/149‑5 (MQ=255)
        aCCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGtt                                                                                                                                        <  2:145165/148‑1 (MQ=255)
        aCCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGtt                                                                                                                                        >  1:145165/1‑148 (MQ=255)
        aCCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTg                                                                                                                                       >  5:118177/1‑149 (MQ=255)
        aCCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGAGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCACGGCGCACCGTGGCGttc                                                                                                                                       >  4:166054/1‑148 (MQ=255)
          cTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTggg                                                                                                                                     <  2:81791/149‑1 (MQ=255)
                       aaaGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTgg                                                                                                                        >  3:18841/1‑149 (MQ=255)
                                tCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGaa                                                                                                                >  3:161565/1‑148 (MQ=255)
                                  cacaCCGATGGTGTTCGGCAGGGCCTGCGGCTGGACATCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAacac                                                                                                             >  1:217428/1‑149 (MQ=255)
                                       cGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGc                                                                                                        <  4:18841/149‑1 (MQ=255)
                                              gtTCGGCAGGGCCTGCGGCTGCACGTCGGCCTTCGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGCACACTTCGCTCTCGc                                                                                                  >  4:183649/1‑148 (MQ=255)
                                                  ggCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCTGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATgg                                                                                             >  6:190406/1‑149 (MQ=255)
                                                  ggCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATgg                                                                                             >  3:215474/1‑149 (MQ=255)
                                                     aGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCt                                                                                          >  1:323398/1‑149 (MQ=255)
                                                              ggCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGa                                                                                 <  2:135241/149‑1 (MQ=255)
                                                                cTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGAcc                                                                               >  7:46395/1‑149 (MQ=255)
                                                                      gTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCagtag                                                                         >  3:169325/1‑149 (MQ=255)
                                                                          gCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGc                                                                     <  4:169325/149‑1 (MQ=255)
                                                                               ggATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGAc                                                                 <  5:74427/148‑1 (MQ=255)
                                                                                      gTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATaacc                                                         <  6:118177/149‑1 (MQ=255)
                                                                                      gTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGATCTCGCCATGGACTTCAAGCAGACCCAGTAGTTGCCGACGGATaac                                                          >  6:96324/1‑148 (MQ=255)
                                                                                            aTCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGaa                                                                                                                >  4:18807/1‑88 (MQ=255)
                                                                                            aTCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGaa                                                                                                                <  3:18807/88‑1 (MQ=255)
                                                                                            aTCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCg                                                   >  4:176184/1‑149 (MQ=255)
                                                                                             tCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCgg                                                  <  4:215474/149‑1 (MQ=255)
                                                                                                   tAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGt                                            >  3:122936/1‑149 (MQ=255)
                                                                                                                         gAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTc                                           <  8:143334/128‑1 (MQ=255)
                                                                                                                         gAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTc                                           >  7:143334/1‑128 (MQ=255)
                                                                                                                                 gtgtATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTgcggc              <  4:161565/149‑1 (MQ=255)
                                                                                                                                 cccaacgCCCCGGTGCGCCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTgcggc              >  1:37062/7‑149 (MQ=255)
                                                                                                                                  ccaacgCCCCGGTGCGCCGGGGCATTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTgcggcg             >  1:198085/6‑149 (MQ=255)
                                                                                                                                   gtATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCa                       <  5:183126/138‑1 (MQ=255)
                                                                                                                                   gtATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCa                       >  6:183126/1‑138 (MQ=255)
                                                                                                                                   caacgCCCCGGTGCGCCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTGcggcgg            >  6:150816/5‑149 (MQ=255)
                                                                                                                                   caacgCCCCGGTGCGCCGGGGCATTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTGcggcgg            <  2:198085/145‑1 (MQ=255)
                                                                                                                                    tATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTGcggcgg            <  8:46395/148‑1 (MQ=255)
                                                                                                                                    tATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTGCGGCggg           <  3:166054/149‑1 (MQ=255)
                                                                                                                                        ccccGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTGCGGCGGGTGTc       <  6:290212/149‑1 (MQ=255)
                                                                                                                                         cccGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTGCGGCGGgt          >  1:982/1‑145 (MQ=255)
                                                                                                                                         cccGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTGCGGCGGgt          <  2:982/145‑1 (MQ=255)
                                                                                                                                            ggCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTGCGGCGGGTGTCGaca   >  8:167082/1‑149 (MQ=255)
                                                                                                                                             gcgcACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTGCGGCGGGTGTCGacac  <  4:122936/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
TTTTTTCCACCTGATTGGCGAACAAAGGGGTGTCCACACCGATGGTGTTCGGCAGGGCCTGCGGCTGGACGTCGGCCTTGGATAGGGTCTGCATCAACATAGCGCTCTCCCCGTGGGTCATGAGCGGTGGTGTATGCCCCGGCGCACCGGGGCGTTGGGCAAGTGTCAGTGGCTGAGGAACACTTCGCTCTCGCCATGGGCTTCAAGCAGACCCAGTAGTTGCCGACGGATAACCAACCCGGGCTTGTCCGATGGCATGTGCAGTTCCACCCTGCGGCGGGTGTCGACAC  >  NC_002947/3644806‑3645095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑