Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,662,976 G→A 13.1% intergenic (‑143/+43) PP_3226 ← / ← PP_3227 acyl‑CoA dehydrogenase‑related protein/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,662,9760GA13.1% 92.3 / 5.5 38intergenic (‑143/+43)PP_3226/PP_3227acyl‑CoA dehydrogenase‑related protein/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (15/18);  new base A (2/3);  total (17/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01

ATCCCTGAATGACGGTGCGTTGCCTAAGACCGTATTCGCATGGATGAAGGCTGTAAAAGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAGGTAGCGCGGATGGAACAGCCACAGATCCACCTGCGGC  >  NC_002947/3662832‑3663121
                                                                                                                                                |                                                                                                                                                  
atgccTGAATGAGGTTGTGGTGTCTATTACCTTTTTCGCTTGGTTGAAGGCTTTATAAGGCTTTTTATGTATATGGTTAATATGTGTTTTAATGATTTAGTGTTTAGGTTGTTATGCGTAATTTTCATTTATAGCTTGGGCTGCGTTGc                                                                                                                                                <  6:199607/146‑1 (MQ=255)
 tCCCTGAATGACGGTGCGTTGCCTAAGACCGTATTCGCATGGATGAAGGCTGTAAAAGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCa                                                                                                                                               <  2:316517/149‑1 (MQ=255)
       aaTGACGGTGCGTTGCCTAAGACCGTATTCGCATGGATGAAGGCTGTAAAAGGCTTTTTATCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCt                                                                                                                                          <  8:208598/148‑1 (MQ=255)
       aaTGACGGTGCGTTGCCTAAGACCGTATTCGCATGGATGAAGGCTGTAAAAGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGACTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCAACGCAGcccaa                                                                                                                                         <  1:124244/149‑3 (MQ=255)
         tGACGGTGCGTTGCCTAAGACCGTATTCGCATGGATGAAGGCTGTAAAAGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTc                                                                                                                                       <  2:9551/149‑1 (MQ=255)
                   ttGCCTAAGACCGTATTCGCATGGATGAAGGCTGTAAAAGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAg                                                                                                                             <  8:1586/149‑1 (MQ=255)
                           gACCGTATTCGCATGGATGAAGGCTGTAAAAGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGACCTTTCGCCACGCAAGGCTCca                                                                                                                       >  5:17335/1‑147 (MQ=255)
                                     gCATGGATGAAGGCTGTAAAAGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAg                                                                                                           >  6:137350/1‑149 (MQ=255)
                                     gCATGGATGAAGGCTGTAAAAGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCAACGCAGcccaag                                                                                                                                        >  5:250475/1‑117 (MQ=255)
                                     gCATGGATGAAGGCTGTAAAAGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCAACGCAGcccaag                                                                                                                                        <  6:250475/120‑4 (MQ=255)
                                               aGGCTGTAAAAGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCc‑aa                                                                                                <  5:137350/149‑1 (MQ=255)
                                                      aaaaGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCc‑a                                                                                                 <  3:143198/141‑1 (MQ=255)
                                                      aaaaGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCc‑a                                                                                                 >  4:143198/1‑141 (MQ=255)
                                                             tttttAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGATTGGGCTGAGTTGCAGCACTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCcg                                                                                  >  7:216428/1‑149 (MQ=255)
                                                                              aTTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGTGCGGCGTATCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCCGAACAACCGATGTCCCGCGACCTCACCaaa                                                                     <  1:189930/146‑1 (MQ=255)
                                                                              aTTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCaaaaa                                                                   <  3:201996/147‑1 (MQ=255)
                                                                                       tttAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGc                                                        >  2:54604/1‑149 (MQ=255)
                                                                                        ttAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCt                                                       >  3:218553/1‑149 (MQ=255)
                                                                                          aaTGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTc                                                      <  5:49360/148‑1 (MQ=255)
                                                                                           aTGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGc                                                                                                                  >  5:166215/1‑88 (MQ=255)
                                                                                           aTGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGc                                                                                                                  <  6:166215/88‑1 (MQ=255)
                                                                                           aTGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCt                                                    >  1:23066/1‑149 (MQ=255)
                                                                                            tGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCATCCCTTTCGCCACGCAAGGCGCCACAGGACAGTGAGATCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGGTCCTg                                                   >  4:190899/1‑149 (MQ=255)
                                                                                              aTTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGt                                                  >  4:120274/1‑148 (MQ=255)
                                                                                                          gCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAgg                                      <  8:216428/148‑1 (MQ=255)
                                                                                                             tGTTATGCGTAATTTACATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAGGTAgc                                  >  7:137578/1‑149 (MQ=255)
                                                                                                               ttATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAGGTAgcgc                                <  2:23066/149‑1 (MQ=255)
                                                                                                                tATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAGGTAgcgc                                <  4:218553/148‑1 (MQ=255)
                                                                                                                    cGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAGGTAGCGCGGATg                           >  4:58406/1‑149 (MQ=255)
                                                                                                                    cGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAGGTAGCGCGGATg                           <  3:120274/149‑1 (MQ=255)
                                                                                                                        aTTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAGGTAGCGCGGATGGaa                        <  1:54604/148‑1 (MQ=255)
                                                                                                                                    cgaaagggCTGCAACGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTaaa                                               <  2:37368/108‑1 (MQ=255)
                                                                                                                                      aaagggCTGCAACGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAGGTAGCGCGGATGGAAAAGCCACAGATCCAc         >  5:274424/4‑149 (MQ=255)
                                                                                                                                       ttGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTAccc                                         >  4:167793/1‑116 (MQ=255)
                                                                                                                                       ttGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTAccc                                         <  3:167793/116‑1 (MQ=255)
                                                                                                                                          ggCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAGGTAGCGCGGATGGAACAGCCACAGATCCACCTGc     >  1:183957/1‑149 (MQ=255)
                                                                                                                                            cTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAGGTAGCGCGGATGGAACAGCCACAGATCCACCTGCgg   <  8:137578/149‑1 (MQ=255)
                                                                                                                                             tGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAGGTAGCGCGGATGGAACAGCCACAGATCCACCTGCGGc  >  8:189501/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
ATCCCTGAATGACGGTGCGTTGCCTAAGACCGTATTCGCATGGATGAAGGCTGTAAAAGGCTTTTTAGCTATATGCTAATTATGAGTTTTAATGATTTAGAGTTTTGCTTGTTATGCGTAATTTGCATTTATAGCTTGGGCTGCGTTGCAGCCCTTTCGCCACGCAAGGCGCCACAGGCCAGTGAGCTCAACCC‑AACAACCGATGTCCCGCGACCTCACCAAAAAGCTCCACCGGCTCCTGTAAATTACCCAGGTAGCGCGGATGGAACAGCCACAGATCCACCTGCGGC  >  NC_002947/3662832‑3663121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑