Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,718,207 C→T 7.1% R129H (CGC→CAC)  paaG ← 2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,718,2070CT 7.1% 166.1 / 2.3 56R129H (CGC→CAC) paaG2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase
Reads supporting (aligned to +/- strand):  ref base C (24/28);  new base T (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

TCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAGAACTTGTCGATCGACTGGCCC  >  NC_002947/3718063‑3718350
                                                                                                                                                |                                                                                                                                               
tCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcgggc                                                                                                                                             >  4:91982/1‑149 (MQ=255)
  ggCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcgggcgg                                                                                                                                           >  7:140449/1‑149 (MQ=255)
  ggCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcgggcgg                                                                                                                                           >  5:246958/1‑149 (MQ=255)
                cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGTGGGCGGCCAGCACCAGGTCg                                                                                                                             >  8:8009/1‑149 (MQ=255)
                cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGTGGGCGGCCAGCACCAGGTCg                                                                                                                             >  8:3374/1‑149 (MQ=255)
                     cgcTCGCCCAGCATGGCCAGCGCCTTGGTCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGc                                                                                                                        <  5:145556/149‑1 (MQ=255)
                          gCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCaa                                                                                                                     <  2:135392/147‑1 (MQ=255)
                           cccAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAgg                                                                                                                   >  4:50840/1‑148 (MQ=255)
                             cAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAgg                                                                                                                   >  8:15203/1‑146 (MQ=255)
                             cAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAgg                                                                                                                   <  7:15203/146‑1 (MQ=255)
                                 aTGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGtt                                                                                                            <  8:140449/149‑1 (MQ=255)
                                  tGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTg                                                                                                           <  4:163256/149‑1 (MQ=255)
                                      cAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTggcac                                                                                                       <  3:50840/149‑1 (MQ=255)
                                      cAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTggcac                                                                                                       >  5:10665/1‑149 (MQ=255)
                                                 cccGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGgcc                                                                                            >  5:41716/1‑149 (MQ=255)
                                                 cccGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGgcc                                                                                            <  6:10665/149‑1 (MQ=255)
                                                 cccGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGgcc                                                                                            >  1:284751/1‑149 (MQ=255)
                                                       ccATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACg                                                                                      >  5:176179/1‑149 (MQ=255)
                                                        cATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGc                                                                                     >  8:303269/1‑149 (MQ=255)
                                                         aTGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGcc                                                                                    >  5:210391/1‑149 (MQ=255)
                                                            ccGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATg                                                                                                              <  3:175943/120‑1 (MQ=255)
                                                            ccGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATg                                                                                                              >  4:175943/1‑120 (MQ=255)
                                                             cGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGtt                                                                                 >  8:73366/1‑148 (MQ=255)
                                                             cGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTg                                                                                >  8:69082/1‑149 (MQ=255)
                                                               aCCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGAc                                                                              <  7:302496/149‑1 (MQ=255)
                                                                 cAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGGACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGGCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCg                                                                            <  8:220177/149‑1 (MQ=255)
                                                                 cAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCg                                                                            >  1:61653/1‑149 (MQ=255)
                                                                            gcagcCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGTGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCAcc                                                                 <  7:3374/149‑1 (MQ=255)
                                                                            gcagcCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGTGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCAcc                                                                 <  7:8009/149‑1 (MQ=255)
                                                                                ccAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGc                                                              >  7:238609/1‑148 (MQ=255)
                                                                                ccAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGc                                                              >  1:10198/1‑148 (MQ=255)
                                                                                  aaGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCaaa                                                           <  2:61653/149‑1 (MQ=255)
                                                                                   aGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAg                                                          >  8:256956/1‑149 (MQ=255)
                                                                                       ccgccgGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCCCCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCac                                                      >  5:104694/1‑148 (MQ=255)
                                                                                        cgccgGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAgg                                                     >  5:64050/1‑149 (MQ=255)
                                                                                          ccgGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTc                                                   >  3:41595/1‑149 (MQ=255)
                                                                                                  cGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGATTGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAggg                                           >  5:192768/1‑149 (MQ=255)
                                                                                                  cGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAgg                                            <  6:176179/148‑1 (MQ=255)
                                                                                                   ggCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGt                                          <  2:284751/149‑1 (MQ=255)
                                                                                                    gCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTg                                         <  3:83176/149‑1 (MQ=255)
                                                                                                          ggCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCAc                                    >  5:287114/1‑148 (MQ=255)
                                                                                                            ccGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAg                                 <  7:18900/149‑1 (MQ=255)
                                                                                                                   caagatcggCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCTAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGtt                           <  3:284598/141‑1 (MQ=255)
                                                                                                                    gCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGATTGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCggg                             <  6:192768/145‑1 (MQ=255)
                                                                                                                    gCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCg                               <  5:130778/143‑1 (MQ=255)
                                                                                                                     cAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGt                         <  7:69082/148‑1 (MQ=255)
                                                                                                                     cAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGt                         <  7:73366/148‑1 (MQ=255)
                                                                                                                       gAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAg                       >  5:7985/1‑148 (MQ=255)
                                                                                                                        aaGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCTGGTTGTAGaa                     <  6:287114/149‑1 (MQ=255)
                                                                                                                        aaGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAGaa                     <  8:238609/149‑1 (MQ=255)
                                                                                                                        aaGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAGaa                     <  4:41595/149‑1 (MQ=255)
                                                                                                                        aaGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAGaa                     <  2:10305/149‑1 (MQ=255)
                                                                                                                             cTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAGAACTTGt                <  6:246958/149‑1 (MQ=255)
                                                                                                                              tGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAGaa                     <  4:305911/143‑1 (MQ=255)
                                                                                                                              tGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAGaa                     >  3:305911/1‑143 (MQ=255)
                                                                                                                                 aTGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAGAACTTGtcgat            >  8:115674/1‑149 (MQ=255)
                                                                                                                                    aaaCTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAGAACTTGtcgatcga         <  6:64050/149‑1 (MQ=255)
                                                                                                                                      aCTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAGAACTTGTCGATCGACt       >  4:9923/1‑149 (MQ=255)
                                                                                                                                           ccGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAGAACTTGTCGATCGACTGGccc  <  7:303269/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCCGCCACGCCGTTGACCGCACATATCACCGGCAAAGGCAGGTCGCGCAGGGTGCGCACCAGCGGGTTGTAGAACTTGTCGATCGACTGGCCC  >  NC_002947/3718063‑3718350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑