| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,821,795 | T→A | 9.3% | D29V (GAC→GTC) ‡ | ptxD ← | phosphonate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,821,795 | 0 | T | A | 9.3% | 143.0 / 5.8 | 54 | D29V (GAC→GTC) ‡ | ptxD | phosphonate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (19/30); new base A (3/2); total (22/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01 | |||||||||||
CACGCCTACCGAAACGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCAGAGGTC > NC_002947/3821650‑3821942 | cACGCCTACCGAAACGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCa < 6:276835/149‑1 (MQ=255) cTACCGAAACGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAg < 1:262900/149‑1 (MQ=255) cTACCGAAACGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAg < 3:81353/149‑1 (MQ=255) ccGAAACGCTCGACACCTCTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGTATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTc < 1:54851/149‑1 (MQ=255) ccGAAACGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGt < 5:93317/148‑1 (MQ=255) ccGAAACGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTc < 2:11271/149‑1 (MQ=255) ccGAAACGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCAAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGACACCA‑CCCAgc < 2:164025/147‑2 (MQ=255) cGAAACGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCCACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CACAGGTCa > 6:243519/1‑149 (MQ=255) cGAAACGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCa > 4:171701/1‑140 (MQ=255) cGAAACGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCa < 3:171701/140‑1 (MQ=255) cGAAACGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCC‑CCCAGGTCa > 5:218176/1‑149 (MQ=255) aaaCGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCAc > 4:31199/1‑148 (MQ=255) gACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGAcgc < 7:34143/148‑1 (MQ=255) caccacTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGAt < 5:328955/149‑1 (MQ=255) accacTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATc > 7:129233/1‑149 (MQ=255) ttCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAGCGCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTg > 1:231219/1‑148 (MQ=255) ttCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGACACCA‑CCCAg < 5:58647/127‑1 (MQ=255) ttCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGACACCA‑CCCAg > 6:58647/1‑127 (MQ=255) ccAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACTACCCGTGCGCCCCGGGTAACGCATCGCGCTGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑GCCAGGTCACCTCGACGCGATCTTGCAg < 2:238720/148‑1 (MQ=255) ccAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCa < 3:58893/147‑1 (MQ=255) cAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGt > 8:233510/1‑126 (MQ=255) cAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGt < 7:233510/126‑1 (MQ=255) aGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCg < 1:1670/149‑1 (MQ=255) gTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGt < 8:215901/149‑1 (MQ=255) gTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGt < 7:193190/149‑1 (MQ=255) cGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCACCCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAgtcgtc < 1:67608/149‑1 (MQ=255) gCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGa > 6:127101/1‑148 (MQ=255) gCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGAc < 2:231219/149‑1 (MQ=255) tGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGt < 3:31199/149‑1 (MQ=255) aTCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTtat < 5:23934/149‑1 (MQ=255) tCCATGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTtata < 4:92994/149‑1 (MQ=255) cAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACa < 4:40143/149‑1 (MQ=255) gcgcAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGa < 5:126081/149‑1 (MQ=255) gCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACCGGACGATTc > 6:77737/1‑149 (MQ=255) gCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGc < 5:117088/147‑1 (MQ=255) ccAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGcttctt > 5:291979/1‑148 (MQ=255) caacCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTg > 4:256464/1‑149 (MQ=255) cccGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAg < 3:256464/149‑1 (MQ=255) gTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCa > 2:262213/1‑59 (MQ=255) cccGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAAt > 3:268876/1‑149 (MQ=255) gCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATgg < 5:127101/149‑1 (MQ=255) gCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATgg > 3:222114/1‑149 (MQ=255) gCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCACCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATgg > 7:238860/1‑149 (MQ=255) gcgcAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGa > 8:34886/1‑149 (MQ=255) cAGGCGGGCCAGGGCATCGGGCTGGCTGGTGTCCC‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGAcgc > 3:131271/1‑149 (MQ=255) aGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGAcgcg < 4:268876/149‑1 (MQ=255) ggCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCACCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGa < 8:238860/149‑1 (MQ=255) gCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAg > 6:200739/1‑149 (MQ=255) ggCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTa < 5:200739/149‑1 (MQ=255) ccAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATc < 4:222114/149‑1 (MQ=255) ggCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCgcgc > 3:292027/1‑149 (MQ=255) gCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCg < 6:291979/149‑1 (MQ=255) cTGGGTGGACACCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCgg > 8:4911/1‑149 (MQ=255) tGGGTGGACACCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGc > 7:89965/1‑149 (MQ=255) gtgtCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCagag < 2:323536/148‑1 (MQ=255) gtCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCAGAGGTc > 7:124707/1‑149 (MQ=255) tCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCttt > 6:229193/1‑133 (MQ=255) tCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCttt < 5:229193/133‑1 (MQ=255) | CACGCCTACCGAAACGCTCGACACCACTTCCAGTTGCGGGGCAAGGTCGAGCAGGCTGGCATCCAGGCGCAG‑GCTGGCCCCCAACAACCCGTGCGCCCCCGGTAACGCATCGCGCAGGCGGGCCAGGGCATCGGGCTGGGTGGTGTCCA‑CCCAGGTCACCTCGACGCGATCTTGCAGGCGTGCCATCAAGTCGTCGGACAGGCGTTTATACAGGACGATTCGCTTCTTCATTGCAGGTTTCCTAATGGTTCATGGCCAGACGCGAGCTATCGCGCTTTGCCCGGCCAGAGGTC > NC_002947/3821650‑3821942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |