Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 329,843 G→A 8.5% A43V (GCC→GTC)  gltR‑I ← two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947329,8430GA 8.5% 127.8 / 2.3 47A43V (GCC→GTC) gltR‑Itwo‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (23/20);  new base A (2/2);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

AGACCGATGATGCGGTCCAGCGTGCTGCCCTTGGCTGACAGCATGATCACTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGCTCATGCAGAAAAACACCTGTTCGGT  >  NC_002947/329696‑329991
                                                                                                                                                   |                                                                                                                                                    
aGACCGATGATGCGGTCCAGCGTGCTGCCCTTGGCTGACAGCATGATCACTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCCCCAGCTCCACTTTGTGCCGGGc                                                                                                                                                     >  6:168231/1‑149 (MQ=255)
aGACCGATGATGCGGTCCAGCGTGCTGCCCTTGGCTGACAGCATGATCACTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGc                                                                                                                                                     <  1:57064/149‑1 (MQ=255)
 gACCGATGATGCGGTCCAGCGTGCTGCCCTTGGCTGACAGCATGATCACTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGcc                                                                                                                                                    <  4:118795/149‑1 (MQ=255)
          tgCGGTCCAGCGTGCTGCCCTTGGCTGACAGCATGATCACTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCa                                                                                                                                           >  3:34222/1‑149 (MQ=255)
                           cccTTGGCTGACAGCATGATCACTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTc                                                                                                                          >  2:121391/1‑149 (MQ=255)
                            ccTTGGCTGACAGCATGATCACTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTct                                                                                                                         <  6:15117/149‑1 (MQ=255)
                                       aGCATGATCACTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCCCGCATCTGTTGACCGTCTGCTGCCGCCAg                                                                                                              >  7:218685/1‑149 (MQ=255)
                                         cATGATCACTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCa                                                                                                            <  7:15433/149‑1 (MQ=255)
                                              tCACTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTg                                                                                                       <  4:34222/149‑1 (MQ=255)
                                              tCACTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTg                                                                                                       >  6:66477/1‑149 (MQ=255)
                                                aCTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTg                                                                                                       >  6:66841/1‑147 (MQ=255)
                                                      aaCCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCgg                                                                                                >  4:15797/1‑148 (MQ=255)
                                                      aaCCAAGGTCTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCgg                                                                                                >  1:245930/1‑148 (MQ=255)
                                                       aCCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTCTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGc                                                                                               >  6:243732/1‑148 (MQ=255)
                                                             gtgtGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGGCGCCAGCACGTTGTAGCCGGCATCGGTc                                                                                        <  2:245930/149‑1 (MQ=255)
                                                              tgtgGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCa                                                                                       <  2:69721/149‑1 (MQ=255)
                                                                       cAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGTCATCGGTCAGGTAATcac                                                                              <  4:10537/149‑1 (MQ=255)
                                                                        aaCTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATcac                                                                              >  3:129480/1‑148 (MQ=255)
                                                                        aaCTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCACCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATcac                                                                              >  7:265006/1‑148 (MQ=255)
                                                                          ctgcctgcACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATcaca                                                                             >  2:110719/1‑147 (MQ=255)
                                                                             cctgcACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAaag                                                                          <  5:66841/147‑1 (MQ=255)
                                                                               tgcACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGt                                                                      >  2:54689/1‑149 (MQ=255)
                                                                                gcACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGACCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATc                                                                                <  4:115222/138‑1 (MQ=255)
                                                                                gcACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGACCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATc                                                                                <  4:119892/138‑1 (MQ=255)
                                                                                gcACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGACCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATc                                                                                >  3:119892/1‑138 (MQ=255)
                                                                                gcACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGACCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATc                                                                                >  3:115222/1‑138 (MQ=255)
                                                                                 cacaGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGTTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTc                                                                    >  8:204455/1‑149 (MQ=255)
                                                                                      gCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTgccgc                                                                                                                 <  6:60510/99‑1 (MQ=255)
                                                                                        tAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGAtc                                                             >  3:128419/1‑149 (MQ=255)
                                                                                        tAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGAtc                                                             >  5:268260/1‑149 (MQ=255)
                                                                                                      cccGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCatcatc                                               <  5:66477/149‑1 (MQ=255)
                                                                                                      cccGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTCGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCatcatc                                               <  2:224005/149‑1 (MQ=255)
                                                                                                       ccGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATAACAAAGAAGTTCGCGGATCTCTTCGtcatca                                                 >  2:88211/1‑146 (MQ=255)
                                                                                                              caTCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAAc                                         >  1:292776/1‑147 (MQ=255)
                                                                                                              caTCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAAc                                         >  8:102051/1‑147 (MQ=255)
                                                                                                                       gAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTc                               <  6:268260/148‑1 (MQ=255)
                                                                                                                            ccaccaGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGTCCGTCTGTTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGcgc                         <  7:204455/149‑3 (MQ=255)
                                                                                                                            ccaccaGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGCt                          >  8:221351/1‑148 (MQ=255)
                                                                                                                                   cTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGCTCATGCAg                   >  8:90283/1‑148 (MQ=255)
                                                                                                                                     ccACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGCTCATGCAGaa                 >  7:124537/1‑148 (MQ=255)
                                                                                                                                      cACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGCTCATGCAGaaa                <  3:15797/148‑1 (MQ=255)
                                                                                                                                       aCTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGCTCATGCAGaaaaa              <  1:121391/149‑1 (MQ=255)
                                                                                                                                        cTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGCTCATGCAGAAAAac             <  1:110719/149‑1 (MQ=255)
                                                                                                                                         tttGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGCTCATGCAGAAAAac             >  5:126585/1‑148 (MQ=255)
                                                                                                                                           tgtgCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGCTCATGCAGAAAAacac           <  5:286514/148‑1 (MQ=255)
                                                                                                                                           tgtgCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGCTCATGCAGAAAAacac           <  1:54689/148‑1 (MQ=255)
                                                                                                                                           tgtgCCGGGCCAGCTGGTCACGCATCTGTTGACCGGCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGCTCATGCAGAAAAacac           <  8:238911/148‑1 (MQ=255)
                                                                                                                                                   gCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGTTAATCACAAAGAAGTTCGCGGATCTTGTCGTCATCATCGACAACCAGCAGGGTCGTGCTCATGCAGAAAAACACCTGTTCGGt  <  1:88211/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGACCGATGATGCGGTCCAGCGTGCTGCCCTTGGCTGACAGCATGATCACTGCCAACCCAGGTGTGGCCTGCAACTGCCTGCACAGGCTAAGGCCGTCCTCACCCGGCAGCATCAGGTCGAGCACCACCAGCTCCACTTTGTGCCGGGCCAGCTGGTCACGCATCTGTTGACCGTCTGCTGCCGCCAGCACGTTGTAGCCGGCATCGGTCAGGTAATCACAAAGAAGTTCGCGGATCTCGTCGTCATCATCGACAACCAGCAGGGTCGTGCTCATGCAGAAAAACACCTGTTCGGT  >  NC_002947/329696‑329991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑