Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 332,461 A→T 8.5% intergenic (+53/+13) PP_0272 → / ← PP_0273 ferric siderophore receptor/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947332,4610AT 8.5% 155.0 / 2.5 55intergenic (+53/+13)PP_0272/PP_0273ferric siderophore receptor/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (23/27);  new base T (3/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCACTACTACAGCCAGTACGGGTTCTATGCCGGGGCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTGCGCCATTGCCACAGGCTAGCGCCTT  >  NC_002947/332319‑332605
                                                                                                                                              |                                                                                                                                                
aaGCACTACTACAGCCAGTACGGGTTCTTTGCCGGGGCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCg                                                                                                                                              >  1:185805/1‑147 (MQ=255)
aaGCACTACTACAGCCAGTACGGGTTCTATGCCGGGGCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCg                                                                                                                                              <  2:185805/147‑1 (MQ=255)
   cACTACTACAGCCAGTACGGGTTCTATGCCGGGGCGATTTATGGCGACCCGCGTAACCTGTCCTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCTTGGCGGccca                                                                                                                                         <  7:169833/149‑2 (MQ=255)
        ctacAGCCAGTACGGGTTCTATGCCGGGGCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTc                                                                                                                                      <  8:50770/147‑1 (MQ=255)
        ctacAGCCAGTACGGGTTCTATGCCGGGGCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCt                                                                                                                                     >  1:180951/1‑148 (MQ=255)
                aGTACGGGGTCTATGCCGGGGCGATTTATGGCGCCCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCATGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGc                                                                                                                            <  6:271337/149‑1 (MQ=255)
                     gggTTCTATGCCGGGGCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTAc                                                                                                                       >  8:278980/1‑149 (MQ=255)
                            aTGCCGGGGCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGc                                                                                                                >  7:151690/1‑149 (MQ=255)
                             tGCCGGGGCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGcc                                                                                                               <  2:134114/149‑1 (MQ=255)
                               ccGGGGCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCt                                                                                                             >  1:311294/1‑149 (MQ=255)
                                cGGGGCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGAGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGAGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTg                                                                                                            >  3:60072/1‑149 (MQ=255)
                                 ggggCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTg                                                                                                            >  3:286056/1‑148 (MQ=255)
                                  gggCGATTTATGACGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGa                                                                                                          <  5:83485/149‑1 (MQ=255)
                                   ggCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCtt                                                                                                                  >  1:162860/1‑140 (MQ=255)
                                   ggCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCtt                                                                                                                  <  2:162860/140‑1 (MQ=255)
                                       aTTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAAc                                                                                                     <  2:311294/149‑1 (MQ=255)
                                       aTTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAAc                                                                                                     <  2:143337/149‑1 (MQ=255)
                                             ggCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCtt                                                                                               >  3:116078/1‑149 (MQ=255)
                                                gACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGTGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTtctc                                                                                            >  7:179364/1‑149 (MQ=255)
                                                   ccGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCaa                                                                                         >  2:206806/1‑149 (MQ=255)
                                                    cgcgTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAAc                                                                                        <  2:180951/149‑1 (MQ=255)
                                                        tAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGc                                                                                    <  5:146868/149‑1 (MQ=255)
                                                         aaCCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGcc                                                                                   >  1:303177/1‑149 (MQ=255)
                                                            cTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTc                                                                                >  3:274913/1‑149 (MQ=255)
                                                               tCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGaa                                                                             <  4:274913/149‑1 (MQ=255)
                                                                 gTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGc                                                                           >  5:174880/1‑149 (MQ=255)
                                                                  ttGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGc                                                                           >  6:13362/1‑148 (MQ=255)
                                                                       ggTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATcc                                                                      >  7:76856/1‑148 (MQ=255)
                                                                            gCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATcc                                                                <  1:206806/149‑1 (MQ=255)
                                                                            gCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTCGGCTCTACCGCTTGCCCTGGATCAACCGGCGTCTCCAAATGGCCCTCGAAGATATCCCAATc                                                                 >  4:127140/1‑148 (MQ=255)
                                                                              aCCGCGTTCTGATCAAACCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGaa                                                                             >  7:25953/1‑134 (MQ=255)
                                                                              aCCGCGTTCTGATCAAACCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGaa                                                                             <  8:25953/134‑1 (MQ=255)
                                                                                    ttCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCaaa                                                        <  8:76856/149‑1 (MQ=255)
                                                                                      cTGATCAAGCCCATTCACAGAACGATCGCGGTGTTCAAGGCCTGCGCTTGGTCGCCAAGGGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGTATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAAc                                                       <  4:60072/148‑1 (MQ=255)
                                                                                          tCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTc                                                  >  4:157322/1‑149 (MQ=255)
                                                                                               cccTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCg                                             <  4:116078/149‑1 (MQ=255)
                                                                                                    ccACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATg                                         >  6:162854/1‑148 (MQ=255)
                                                                                                     cacaGAACCAGCGCTGGGTTCAAGGCCTTCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGc                                        <  8:179364/148‑1 (MQ=255)
                                                                                                       caGATCCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGGGGCCCTTTCTTACCTGGTCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCATCTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCa                                       <  5:327523/147‑1 (MQ=255)
                                                                                                       caGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATg                                     <  5:162854/149‑1 (MQ=255)
                                                                                                       caGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATg                                     <  2:303177/149‑1 (MQ=255)
                                                                                                          aaCCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTc                                  <  3:157322/149‑1 (MQ=255)
                                                                                                            ccAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCg                                <  6:174880/149‑1 (MQ=255)
                                                                                                                 gcTGTGTTCAAGGCCTGCGGTGGGCCGTCTAGCGGCACTTTCTTACCTGTGCTCTACCTCTTGCCCTGGATCAACCGGCTTATCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTg                           <  6:328188/149‑1 (MQ=255)
                                                                                                                          aaGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTGCGCCATTGc                  <  4:286056/149‑1 (MQ=255)
                                                                                                                                  gaaagggCCGCTTGGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTGCGCCATTGCCACAGGc           >  7:231270/5‑148 (MQ=255)
                                                                                                                                     tGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTGCGCCATTGCCACAGGCTAgc       <  8:151690/149‑1 (MQ=255)
                                                                                                                                     agggCCGCTTGGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTGCGCCATTGCCACAGGCTAgc       >  5:68726/2‑149 (MQ=255)
                                                                                                                                     agggCCGCTTGGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTGCGCCATTGCCACAGGCTAgc       >  6:65385/2‑149 (MQ=255)
                                                                                                                                       ggCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTGCGCCGTTGCCACAGGCTAgt       <  5:13362/147‑2 (MQ=255)
                                                                                                                                        gccgccAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTGCGCCATTGCCACAGGCTAGCGcc    >  8:296479/1‑149 (MQ=255)
                                                                                                                                        gccgcaAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCAtc                                     >  2:113195/1‑115 (MQ=255)
                                                                                                                                          cgccAATCGGCCCTTTCTTACCTGGGCTCTACGGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTGCGCCATTGCCACAGGCTAGCGCCtt  <  3:127140/149‑1 (MQ=255)
                                                                                                                                          cgccAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTGCGCCATTGCCACAGGCTAGCGCCtt  <  7:278980/149‑1 (MQ=255)
                                                                                                                                          cgccAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTGCGCCATTGCCACAGGCTAGCGCCtt  >  2:38645/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                
AAGCACTACTACAGCCAGTACGGGTTCTATGCCGGGGCGATTTATGGCGACCCGCGTAACCTGTCGTTGACGGTAAGCACCGCGTTCTGATCAAGCCCTTCCACAGAACCAGCGCTGGGTTCAAGGCCTGCGCTGGGCCGCCAAGCGGCCCTTTCTTACCTGGGCTCTACCGCTTGCCCTGGATCAACCGGCTTCTCCAACTGGCCCTCGAAGCTATCCCAATCCTCGCCAAACAGCTCCACCGGATGCATGGTCCGGTCTGCGCCATTGCCACAGGCTAGCGCCTT  >  NC_002947/332319‑332605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑