Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,405,320 T→C 8.9% M81V (ATG→GTG)  PP_3886 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,405,3200TC 8.9% 119.1 / 2.9 45M81V (ATG→GTG) PP_3886hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/20);  new base C (2/2);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

CCGATAGCCAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAGCTGG  >  NC_002947/4405172‑4405462
                                                                                                                                                    |                                                                                                                                               
ccGATAGCCAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCAt                                                                                                                                                 >  8:12094/1‑149 (MQ=255)
       ccAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGa                                                                                                                                          <  7:65650/149‑1 (MQ=255)
        cAGCGATCTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCTTGACGTAATTTGGAGGGACTGGGCATAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGTCATGTGCCGa                                                                                                                                          <  7:121851/148‑1 (MQ=255)
            gAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCtt                                                                                                                                    <  5:268768/149‑1 (MQ=255)
            gAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCtt                                                                                                                                    <  7:12094/149‑1 (MQ=255)
                  ccTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCTCGGCACATGCCGGG‑GCtttttcg                                                                                                                               <  2:179499/148‑6 (MQ=255)
                   cTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACTGAATTTGGAGGGACTGGGCTGAAATCTGAGCATCTAACGTCTCGACGCTTTCGATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATgg                                                                                                                             <  5:252214/149‑1 (MQ=255)
                   cTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATgg                                                                                                                             <  2:72538/149‑1 (MQ=255)
                   cTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATgg                                                                                                                             >  7:37541/1‑149 (MQ=255)
                      ggAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTg                                                                                                                          <  6:66997/149‑1 (MQ=255)
                           aGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGAt                                                                                                                     <  6:37263/149‑1 (MQ=255)
                           aGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGAt                                                                                                                     >  3:286273/1‑149 (MQ=255)
                            gggCGACCCGACTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATg                                                                                                                    <  2:159251/149‑1 (MQ=255)
                             ggCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGTGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCTCGGCACATGCCGGG‑Gc                                                                                                                                      >  6:330913/1‑130 (MQ=255)
                             ggCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGTGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCTCGGCACATGCCGGG‑Gc                                                                                                                                      <  5:330913/130‑1 (MQ=255)
                                gACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCaa                                                                                                                >  8:9514/1‑149 (MQ=255)
                                 aCCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAAc                                                                                                               <  7:9514/149‑1 (MQ=255)
                                    cGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCt                                                                                                            >  3:300980/1‑149 (MQ=255)
                                              cgATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGc                                                                                                  <  3:114096/149‑1 (MQ=255)
                                              cgATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGc                                                                                                  >  4:114096/1‑149 (MQ=255)
                                                   aCCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAGTGCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTc                                                                                              <  1:61854/149‑1 (MQ=255)
                                                    cccGTGACGGAATTTGCAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGt                                                                                            >  1:302510/1‑149 (MQ=255)
                                                    accGTGACGGTATTTGGAGGGACTGGGCAGAAATCTTAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGt                                                                                            <  4:286273/148‑1 (MQ=255)
                                                       gTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGgc                                                                                         >  2:3387/1‑149 (MQ=255)
                                                            ggAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCACCCCTGTCACGTCGCTGTCGAGGCGCTTc                                                                                    >  8:237639/1‑149 (MQ=255)
                                                            ggAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTc                                                                                    >  2:24787/1‑149 (MQ=255)
                                                            ggAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCATGGAATGGGTGGAGATGGCAAACCTGTCAGGTCGCTGTCGTGGCGCTTc                                                                                    >  5:69736/1‑149 (MQ=255)
                                                                tttGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTc                                                                                <  8:37541/149‑1 (MQ=255)
                                                                                       tGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTAcc                                                         >  2:57223/1‑149 (MQ=255)
                                                                                                aaCGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTc                                                                           <  8:177239/122‑1 (MQ=255)
                                                                                                aaCGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTc                                                                           >  7:177239/1‑122 (MQ=255)
                                                                                                aaCGTCTCGAAGCTATCCATGACTGGCTACATTTAACGAAAAATCACCGGCATGTGCCGAG‑GCTTGGAATGTGTGGAGATGGAAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCATCCAGTCCCCACGCTGACTGTTAAACCTGCACGt                                                >  6:181725/1‑149 (MQ=255)
                                                                                                 aCGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGtt                                               >  3:255281/1‑149 (MQ=255)
                                                                                                               tCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCttt                                  <  1:24787/148‑1 (MQ=255)
                                                                                                                  aTGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatc                              <  4:300980/149‑1 (MQ=255)
                                                                                                                  aTGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGat                               >  6:266208/1‑148 (MQ=255)
                                                                                                                    gACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGatcat                            >  5:44699/1‑149 (MQ=255)
                                                                                                                           tCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCaa                      >  1:63715/1‑148 (MQ=255)
                                                                                                                           tCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCaa                      >  4:100498/1‑148 (MQ=255)
                                                                                                                            ccATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCaaa                     >  2:204599/1‑148 (MQ=255)
                                                                                                                              aTTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAAc                  <  4:255281/149‑1 (MQ=255)
                                                                                                                                     gAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAg           <  2:63715/149‑1 (MQ=255)
                                                                                                                                         aaGCCTCGGCACATGCCGGG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGcc         >  4:161714/1‑147 (MQ=255)
                                                                                                                                             cccGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCGGcca   >  2:267606/1‑147 (MQ=255)
                                                                                                                                             cccGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCACCAAAAACGACAAAGCCCACCTg   >  7:156451/1‑149 (MQ=255)
                                                                                                                                              ccGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAGCTgg  <  1:57223/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CCGATAGCCAGCGAACTGCCTGGGAACAGGGCGACCCGAGTTCCGCCGATGACCCGTGACGGAATTTGGAGGGACTGGGCAGAAATCTGAGCATCTAACGTCTCGACGCTATCCATGACTGGCTCCATTTAACGAAAAAGCCCCGGCATGTGCCGAG‑GCTTGGAATGGGTGGAGATGGCAACCCTGTCAGGTCGCTGTCGTGGCGCTTCCCTCCAGTCCCCACGCTGACTGTTACCCCTGCACGTTGCCGCCGGGCTTTGATCATCTCCAAAAACGACAAAGCCCAGCTGG  >  NC_002947/4405172‑4405462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑