Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,411,323 C→T 6.0% intergenic (‑183/+142) PP_3894 ← / ← PP_3895 replication protein O/regulatory protein Cro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,411,3230CT 6.0% 200.9 / 2.1 66intergenic (‑183/+142)PP_3894/PP_3895replication protein O/regulatory protein Cro
Reads supporting (aligned to +/- strand):  ref base C (34/28);  new base T (2/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

GACACACTCACGACGGAGTGCGGCTTTCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCACGCATAATTCATCTCGTCAGGC  >  NC_002947/4411176‑4411470
                                                                                                                                                   |                                                                                                                                                   
gACACACTCACGACGGAGTGCGGCTTTCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGc                                                                                                                                                     <  4:56471/148‑1 (MQ=255)
   acacTCACGACGGAGTGCGGCTTTCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGc                                                                                                                                                     >  1:125253/1‑145 (MQ=255)
   acacTCACGACGGAGTGCGGCTTTCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGc                                                                                                                                                     <  2:125253/145‑1 (MQ=255)
           gacgGAGTGCGGCTTTCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTc                                                                                                                                         >  7:59379/1‑149 (MQ=255)
               gAGTGCGGCTTTCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGAt                                                                                                                                      >  5:112172/1‑148 (MQ=255)
                aGTGCGGCTTTCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGAtt                                                                                                                                     >  7:184008/1‑148 (MQ=255)
                  tGCGGCTTTCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAAc                                                                                                                                  <  5:250411/149‑1 (MQ=255)
                   gCGGCTTTCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGTATAATCAACCTCGATTAAct                                                                                                                                 >  5:32054/1‑149 (MQ=255)
                   gCGGCTTTCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGTATAATCAACCTCGATTAAct                                                                                                                                 >  5:27421/1‑149 (MQ=255)
                       cTTTCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATCAActgctg                                                                                                                             <  4:286784/149‑1 (MQ=255)
                          tCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGTATAATCAACCTCGATTAACTGCTGtt                                                                                                                           <  6:27421/148‑1 (MQ=255)
                          tCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGTATAATCAACCTCGATTAACTGCTGtt                                                                                                                           <  6:32054/148‑1 (MQ=255)
                          tCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAActgctg                                                                                                                             >  7:51531/1‑146 (MQ=255)
                          tCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGtt                                                                                                                           <  8:184008/148‑1 (MQ=255)
                            gAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTgaa                                                                                                                        <  2:314808/149‑1 (MQ=255)
                                  gCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGcc                                                                                                                  >  5:158977/1‑149 (MQ=255)
                                   cTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCg                                                                                                                 >  2:227414/1‑149 (MQ=255)
                                           tGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCa                                                                                                         >  4:98086/1‑149 (MQ=255)
                                           tGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCa                                                                                                         >  3:178648/1‑149 (MQ=255)
                                            ggAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCAc                                                                                                        >  1:70665/1‑149 (MQ=255)
                                            ggAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCAc                                                                                                        >  1:1928/1‑149 (MQ=255)
                                                gCGAATCATTCGCGCGGCATGAAGTGCGGCGGATATGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCgg                                                                                                    >  1:44721/1‑149 (MQ=255)
                                                  gAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGtt                                                                                                  >  8:183696/1‑149 (MQ=255)
                                                       aTTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGtttttt                                                                                              >  7:284316/1‑148 (MQ=255)
                                                           gcgcgGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATg                                                                                          <  8:261315/148‑1 (MQ=255)
                                                             gcgGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGAGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCt                                                                                       <  5:274902/149‑1 (MQ=255)
                                                                 cATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCaa                                                                                   >  3:290772/1‑149 (MQ=255)
                                                                     aaGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCa                                                                               <  3:98086/149‑1 (MQ=255)
                                                                       gTGCGGCTGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAg                                                                              <  8:59379/148‑1 (MQ=255)
                                                                       gTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAgg                                                                             <  6:158977/149‑1 (MQ=255)
                                                                          cggcggATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTAc                                                                          >  6:228168/1‑149 (MQ=255)
                                                                           ggcggATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGtttttt                                                                                              <  4:213094/128‑1 (MQ=255)
                                                                            gcggATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTg                                                                        >  4:281813/1‑149 (MQ=255)
                                                                                 tttGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACg                                                                    >  5:312191/1‑148 (MQ=255)
                                                                                    gtgGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCTTAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGAt                                                                 >  5:189326/1‑148 (MQ=255)
                                                                                    gtgGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGa                                                                      >  1:162550/1‑143 (MQ=255)
                                                                                    gtgGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGa                                                                      <  2:162550/143‑1 (MQ=255)
                                                                                     tgGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGa                                                               <  3:229783/149‑1 (MQ=255)
                                                                                      ggAAGGCTTTCTGGTGTGAAAGGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCTACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGa                                                               <  1:227414/148‑1 (MQ=255)
                                                                                      ggAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACGCGGTTTTTTTA‑GGCTGCAAATAAGGTTATGGGCggggatcc                                                             >  5:278703/1‑143 (MQ=255)
                                                                                       gAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTAAGGTACTGGACGGATGaa                                                              >  5:178777/1‑148 (MQ=255)
                                                                                           gCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCTTAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAAcagca                                                         <  6:189326/149‑1 (MQ=255)
                                                                                                     gtgAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGtct                                                >  3:25687/1‑148 (MQ=255)
                                                                                                     gtgAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGtct                                                >  5:326057/1‑148 (MQ=255)
                                                                                                      tgAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCa                                              <  2:70665/149‑1 (MQ=255)
                                                                                                      tgAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGAAATTCAGGTACTGGACGGATTCACAGCAGGACGGTCTCa                                              >  8:130369/1‑149 (MQ=255)
                                                                                                        aaaCGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCAGCCGGTTTTTTTATGCCTTCAATTCAGGTACTGGACGGATGAACAGCAGGACGTTGTCac                                             <  8:51531/148‑1 (MQ=255)
                                                                                                        aaaCGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCac                                             >  7:62336/1‑148 (MQ=255)
                                                                                                          aCGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCactac                                          >  4:253332/1‑149 (MQ=255)
                                                                                                             gCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTgg                                       >  7:249807/1‑149 (MQ=255)
                                                                                                              ccAAGGGTGCAGTGTTGCTTGCATCCATT‑CAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGgcg                                     >  4:80944/1‑149 (MQ=255)
                                                                                                                   ggTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAAt                                 <  8:62336/149‑1 (MQ=255)
                                                                                                                   ggTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAAt                                 <  3:253332/149‑1 (MQ=255)
                                                                                                                   ggTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAAt                                 <  4:290772/149‑1 (MQ=255)
                                                                                                                    gTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAAt                                 <  3:281813/148‑1 (MQ=255)
                                                                                                                     tGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGTCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGc                               <  4:25687/149‑1 (MQ=255)
                                                                                                                     tGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAg                                                                              <  8:291094/102‑1 (MQ=255)
                                                                                                                     tGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAg                                                                              >  7:291094/1‑102 (MQ=255)
                                                                                                                     tGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGc                               >  8:32074/1‑149 (MQ=255)
                                                                                                                          tgtTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGc                               >  2:263382/1‑144 (MQ=255)
                                                                                                                            ttgcttgcATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGG‑TTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCAc                       >  7:239853/1‑149 (MQ=255)
                                                                                                                               cttgcATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCACg                      <  2:44721/148‑1 (MQ=255)
                                                                                                                               cttgcATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCACGc                     <  2:1928/149‑1 (MQ=255)
                                                                                                                                  gcATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCACGCa                    <  6:312191/147‑1 (MQ=255)
                                                                                                                                    aTCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCACGCATAAt                <  1:263382/149‑1 (MQ=255)
                                                                                                                                        aTTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCACGCATAATTCa             >  3:14045/1‑148 (MQ=255)
                                                                                                                                               agTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCACACATAATTCATCTCGTCa     <  6:112172/149‑1 (MQ=255)
                                                                                                                                                 tGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCACGCATAATTCATCTCGTCAgg   >  7:77297/1‑149 (MQ=255)
                                                                                                                                                 tGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCACGCATAATTCATCTCGTCAgg   <  8:249807/149‑1 (MQ=255)
                                                                                                                                                   cATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCACGCATAATTCATCTCGTCAGGc  <  6:326057/148‑1 (MQ=255)
                                                                                                                                                   cATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCACGCATAAGTCATCTCGTCAGGc  >  5:230888/1‑148 (MQ=255)
                                                                                                                                                   cATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGACCACGCATAATTCATCTCGTCAGGc  <  8:284316/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GACACACTCACGACGGAGTGCGGCTTTCGAGGTGGCTGCGTACTGGAGGCGAATCATTCGCGCGGCATGAAGTGCGGCGGATTTGTGGAAGGCTTTCTGGTGTGAAACGGCCAAGGGTGCAGTGTTGCTTGCATCCATTGCAGAGTGCATAATCAACCTCGATTAACTGCTGTTGAAGAAGCCGGGCTGCCACCCGGTTTTTTTATGCCTGCAATTCAGGTACTGGACGGATGAACAGCAGGACGGTCTCACTACTGGCGCAATGCCAGATCACGCATAATTCATCTCGTCAGGC  >  NC_002947/4411176‑4411470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑