Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,463,959 A→T 19.0% intergenic (+6/+22) PP_3956 → / ← betT‑III hypothetical protein/choline/carnitine/betaine transporter BetTC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,463,9590AT19.0% 67.9 / 13.1 37intergenic (+6/+22)PP_3956/betT‑IIIhypothetical protein/choline/carnitine/betaine transporter BetTC
Reads supporting (aligned to +/- strand):  ref base A (15/15);  new base T (4/3);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

CTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACTTCCGC  >  NC_002947/4463818‑4464106
                                                                                                                                             |                                                                                                                                                   
cTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGc                                                                                                                                               <  8:290297/148‑1 (MQ=255)
 tACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGTCCTGCCCTGTCGCCGTCAGGGCAg                                                                                                                                             >  3:11827/1‑149 (MQ=255)
 tACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGTCCTGCCCTGTCGCCGTCAGGGCAg                                                                                                                                             >  3:7474/1‑149 (MQ=255)
    cAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGTCCTGCCCTGTCGCCGTCAGGGCAgggc                                                                                                                                          <  4:7474/149‑3 (MQ=255)
    cAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGTCCTGCCCTGTCGCCGTCAGGGCAgggc                                                                                                                                          <  4:11827/149‑3 (MQ=255)
    cAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATAGGTTAGGACCGCCGAGCGACCGCATTGCTGCAGTAGACTTCTGGGACTGTCAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTCCCCTGCCGGCGACACGGCAggcc                                                                                                                                          >  8:142069/1‑147 (MQ=255)
        tCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGaa                                                                                                                                      <  7:165993/149‑1 (MQ=255)
            cACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATc                                                                                                                                    <  5:50836/147‑1 (MQ=255)
            cACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATc                                                                                                                                    <  3:246968/147‑1 (MQ=255)
               gACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACtt                                                                                                                               >  3:244443/1‑149 (MQ=255)
                    aCGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGcc                                                                                                                          >  6:216086/1‑149 (MQ=255)
                                        tATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATg                                                                                                        >  2:258174/1‑147 (MQ=255)
                                                   aCCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCg                                                                                           <  1:258174/149‑1 (MQ=255)
                                                       ccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACt                                                                                       >  2:258191/1‑149 (MQ=255)
                                                              aaCGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAg                                                                                >  6:121794/1‑149 (MQ=255)
                                                               aCGCATTGCTGCAGTAGACTTCTGGTCCTTTGAATATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGAGGGCGACAGGGCAGGACCGACTCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAgg                                                                               <  7:142069/149‑1 (MQ=255)
                                                                cacATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGa                                                                              >  6:193662/3‑149 (MQ=255)
                                                                       ctgcAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGc                                                                        >  3:227337/1‑148 (MQ=255)
                                                                                aCTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATc                                                              <  4:244443/149‑1 (MQ=255)
                                                                                  ttCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGt                                                            >  7:183280/1‑149 (MQ=255)
                                                                                                 aaTCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCa                                             <  5:193662/149‑1 (MQ=255)
                                                                                                   tCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCat                                            >  4:211151/1‑148 (MQ=255)
                                                                                                        cTTTTGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCGACCACGTGCAAGTACTGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTc                                      <  8:104616/149‑1 (MQ=255)
                                                                                                        cTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTc                                      <  2:312194/149‑1 (MQ=255)
                                                                                                            ggACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAg                                  >  8:96098/1‑149 (MQ=255)
                                                                                                             gACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAg                                  <  4:273037/148‑1 (MQ=255)
                                                                                                                    ggCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGcc                          >  7:68394/1‑149 (MQ=255)
                                                                                                                     gCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGccc                         <  7:88450/149‑1 (MQ=255)
                                                                                                                         aCCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCa                                                          <  4:185403/112‑1 (MQ=255)
                                                                                                                         aCCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCa                                                          >  3:185403/1‑112 (MQ=255)
                                                                                                                          ccGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTtc                    <  1:258191/149‑1 (MQ=255)
                                                                                                                           ggtcctgccctgTCGCCGTCAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTtct                   >  7:245636/4‑149 (MQ=255)
                                                                                                                            gtcctgccctgTCGCCGTCAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTtctc                  <  8:245636/147‑1 (MQ=255)
                                                                                                                            gccctgccctGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTtctc                  <  8:183280/149‑1 (MQ=255)
                                                                                                                             ccctgccctgACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCg                 >  3:1879/1‑149 (MQ=255)
                                                                                                                                   cctgTCGCCGTCAGGGCAGGGCCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATg           >  4:46500/1‑149 (MQ=255)
                                                                                                                                            gACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACTTCcgc  >  5:81416/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACTTCCGC  >  NC_002947/4463818‑4464106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑