| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,496,668 | A→G | 14.9% | intergenic (+18/+34) | PP_3987 → / ← PP_5651 | site‑specific recombinase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,496,668 | 0 | A | G | 14.9% | 59.2 / 4.2 | 28 | intergenic (+18/+34) | PP_3987/PP_5651 | site‑specific recombinase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (11/12); new base G (2/2); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01 | |||||||||||
CCCCAAGGCGGCAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGACCTTG > NC_002947/4496527‑4496808 | ccccAAGGCGGCAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTccc < 8:104654/149‑1 (MQ=255) cAAGGCGGCAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGc < 5:140489/149‑1 (MQ=255) aaGGCGGCAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCt > 7:82331/1‑149 (MQ=255) ggcggcAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGAGCGTACCCCGCttc < 7:69115/149‑2 (MQ=255) ggcggcAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGAGCGTACCCCGCttc < 6:228751/149‑2 (MQ=255) gCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAACCGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACaa > 5:224133/1‑149 (MQ=255) aTCGGCGAGTGGGGTCACGCACGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTcac < 6:224133/149‑1 (MQ=255) aGCCGCCTATTGGGTCACACTGACAAGCTCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATAc < 3:98484/149‑1 (MQ=255) tATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTAACAACTGATCTATTGAGCTATACGCATTg > 3:176778/1‑148 (MQ=255) cacTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTa < 7:63390/149‑1 (MQ=255) aCAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTtg < 8:82331/149‑1 (MQ=255) cATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGctct > 4:294948/1‑149 (MQ=255) aTCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTg > 3:200971/1‑149 (MQ=255) acacaAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCa > 8:42830/1‑149 (MQ=255) cacaAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCAc > 4:162382/1‑149 (MQ=255) aaaCGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATACACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCAccc > 4:102693/1‑148 (MQ=255) aCGTTATATAGAGGAGTAGGTGATATCTTACAGCCAACACGATAGGTAATTCGAAGCGTGGTTCGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACGCAat < 8:290378/149‑1 (MQ=255) cGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTAGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAatc < 3:102693/149‑1 (MQ=255) tataGACTAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATcatcat < 3:237091/149‑1 (MQ=255) aGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGctct < 7:205051/130‑1 (MQ=255) aGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGctct > 8:205051/1‑130 (MQ=255) gTAGGTGAAATCGTACATCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCga < 3:162382/149‑1 (MQ=255) tGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGaa > 1:189284/1‑149 (MQ=255) cAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCt < 3:294948/149‑1 (MQ=255) aTTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTg < 4:200971/148‑1 (MQ=255) ttCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGa > 2:157733/1‑149 (MQ=255) tcaaaGCGGGGAGCGTACCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACATCGCACGCCTGAGTAGCTCTTTTGAc > 4:43094/4‑149 (MQ=255) aGCGGGGAGCGTACCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGACCTTg > 7:156154/1‑149 (MQ=255) | CCCCAAGGCGGCAAGCGAAATCGGCGATTTGGGTCACGCAAGCCGCCTATTGGGTCACACTGACAAGCGCATCACACAAACGGTATATAGACGAGTAGGTGAAATCGTACAGCCAACACGATAGGTAATTCGAAGCGGGGTACGCTCCCCGCTTTGAGCACAATTCACAACTGATCTATTGAGCTATACGCATTGTATCAATCTTAGCTTGCTGCTCTGCCACCCAATCATCATAGGTTTCGAGAGAATACCTCGCACGCCTGAGTAGCTCTTTGGACCTTG > NC_002947/4496527‑4496808 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |