Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,527,403 T→G 8.3% V150G (GTC→GGC)  purB → adenylosuccinate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,527,4030TG 8.3% 162.5 / 5.2 57V150G (GTC→GGC) purBadenylosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base T (29/23);  new base G (2/3);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCCGCCAAGCTGCCTGAGCTGGCCAAGGTCAGCGAGTTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCGTGTACCGCCTGGAGCGCCAGATCGCCCAGGTT  >  NC_002947/4527262‑4527548
                                                                                                                                             |                                                                                                                                                 
aGGCCGCCAAGCTGCCTGAGCTGGCCAAGGTCAGCGAGTTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGGCGCGGat                                                                                                                                            >  4:249777/1‑148 (MQ=255)
  gccgccAAGCTGCCTGAGCTGGCCAAGGTCAGCGAGTTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGc                                                                                                                                          >  2:325437/1‑149 (MQ=255)
        aaGCTGCCTGAGCTGGCCAAGGTCAGCGAGTTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCcg                                                                                                                                    >  7:28275/1‑149 (MQ=255)
               cTGAGCTGGCCAA‑GTCAGCGAGTTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTgg                                                                                                                            >  1:169633/1‑149 (MQ=255)
                  aGCTGGCCAAGGTCAGCGAGTTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGGCGCGGATGGCGTCGGCGACCTGgc                                                                                                                           <  3:249777/148‑1 (MQ=255)
                            ggTCAGCGAGTTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGcccacgcccacg                                                                                                                <  5:64093/149‑1 (MQ=255)
                                  cGAGTTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAg                                                                                                          >  6:192826/1‑149 (MQ=255)
                                  cGAGTTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAg                                                                                                          >  2:138805/1‑149 (MQ=255)
                                    aGTTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTg                                                                                                        <  5:192826/149‑1 (MQ=255)
                                     gTTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGc                                                                                                       <  6:8115/149‑1 (MQ=255)
                                         aTCCTCTTCGCCTGCACCATCGAGGACATCACCAACGTGTCCCACGCCCTGATGCTGCGCGCCGTCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACTCCATCCGCGCCCTGGCCCACGCACACGCCTAAGTGCCAAt                                                                                                   <  2:156965/149‑1 (MQ=255)
                                          tCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATg                                                                                                  >  4:179750/1‑149 (MQ=255)
                                             aCTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATg                                                                                                  >  5:203884/1‑146 (MQ=255)
                                                 cGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTcg                                                                                            <  2:169633/148‑1 (MQ=255)
                                                   ccTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTcgcg                                                                                          >  2:168080/1‑148 (MQ=255)
                                                       cACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCAc                                                                                       <  5:261283/147‑1 (MQ=255)
                                                          cAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACg                                                                                  >  2:208972/1‑149 (MQ=255)
                                                           aGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACgg                                                                                 <  1:168080/149‑1 (MQ=255)
                                                                  aCATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCACACGCCCACGCCGAACTGCCAATCCTGTCGCGCACCCACGGCCAGCCg                                                                          >  8:268780/1‑149 (MQ=255)
                                                                   cATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCgg                                                                         <  1:253682/149‑1 (MQ=255)
                                                                    aTCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGt                                                                                              <  6:158741/127‑1 (MQ=255)
                                                                    aTCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGt                                                                                              >  5:158741/1‑127 (MQ=255)
                                                                    aTCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGc                                                                        >  1:130133/1‑149 (MQ=255)
                                                                    aTCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGc                                                                        >  1:296599/1‑149 (MQ=255)
                                                                    aTCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGc                                                                        <  2:44747/149‑1 (MQ=255)
                                                                       aacaacCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGctctttac                                                                     >  7:139245/1‑142 (MQ=255)
                                                                       aacaacCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTc                                                                     >  3:308032/1‑149 (MQ=255)
                                                                       aacaacCGGTCCCACGCCCTGATGCTGCCCGCCGGCCGAGACGAAGTGCTGCTGCCGCTGCTGCGCCAGATCGCCGACGCCATACGCGCCCTGGCCCAAGCCCACGAGGCAGTGCCAATGCTGAAGCGCAACCACGGCCCGCCAGCTTc                                                                     >  6:253793/1‑149 (MQ=255)
                                                                        acaacCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGTTGTCGCGCACCCACGGCCAGCCGGCTTCg                                                                    <  7:164242/149‑1 (MQ=255)
                                                                        acaacCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGTTGTCGCGCACCCACGGCCAGCCGGCTTCg                                                                    >  8:164242/1‑149 (MQ=255)
                                                                         caacCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCAc                                                                                   <  5:218404/133‑1 (MQ=255)
                                                                         caacCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCAc                                                                                   >  6:218404/1‑133 (MQ=255)
                                                                                  ccACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGa                                                                >  4:94794/1‑143 (MQ=255)
                                                                                  ccACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGATGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGa                                                                <  3:94794/143‑1 (MQ=255)
                                                                                   cACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCt                                                         >  5:18038/1‑149 (MQ=255)
                                                                                    aCGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTg                                                        >  5:312915/1‑149 (MQ=255)
                                                                                     cGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCGCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTgg                                                       <  6:203884/149‑1 (MQ=255)
                                                                                              gctgcGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGc                                              >  7:170960/1‑149 (MQ=255)
                                                                                                  cgcgcCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTgg                                           <  5:173601/148‑1 (MQ=255)
                                                                                                  cgcgcCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTgg                                           <  1:325437/148‑1 (MQ=255)
                                                                                                  cgcgcCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGc                                          <  4:308032/149‑1 (MQ=255)
                                                                                                        ggccgTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAAcgt                                    >  4:268754/1‑149 (MQ=255)
                                                                                                          ccgTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAAcgtcg                                  <  6:18038/149‑1 (MQ=255)
                                                                                                             tGACGAAGTGCTGCTGCCGCTGATGCGCCAGGGCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCgtgt                               <  8:127760/149‑1 (MQ=255)
                                                                                                              gACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCGTGTa                              >  3:261368/1‑149 (MQ=255)
                                                                                                                 gAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCGTGTACCg                           >  7:11698/1‑149 (MQ=255)
                                                                                                                  aaGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCGTGTACCGc                          <  2:296599/149‑1 (MQ=255)
                                                                                                                       ctgctgcCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCGTGTACCGCCTg                       >  7:7135/1‑147 (MQ=255)
                                                                                                                          ctgcCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCGTGTACCGCCTGGAgcg                  >  4:260516/1‑149 (MQ=255)
                                                                                                                           tgcCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCGTGTACCGCCTGGAgcgc                 >  8:215696/1‑149 (MQ=255)
                                                                                                                                   aTGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCGTGTACCGCCTGGAGCGCCAGATCg          <  7:215696/148‑1 (MQ=255)
                                                                                                                                   aTGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCGTGTACCGCCTGGAGCGCCAGATCg          <  8:170960/148‑1 (MQ=255)
                                                                                                                                   aTGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCACACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGATGGCCAACGTCGTGTACAGCCTGCACCGCCAGATAGc         >  4:157737/1‑149 (MQ=255)
                                                                                                                                      cgcCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCGTGTACCGCCTGGAGCGCCAGATCGccc       <  3:268754/148‑1 (MQ=255)
                                                                                                                                         cAGGGCGCGGATGGCGTCGGCGACCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACg                                                                                  >  3:181466/1‑70 (MQ=18)
                                                                                                                                         cAGGGCGCGGATGGCGTCGGCGACCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACg                                                                                  <  4:181466/70‑1 (MQ=18)
                                                                                                                                          aGGTCGCCGACGCCATCCGCGCCCTGGCCCACGGCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCGTGTACCGCCTGGAGCGCCAGATCGCCCAGGtt  <  7:268780/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
AGGCCGCCAAGCTGCCTGAGCTGGCCAAGGTCAGCGAGTTCATCCACTTCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGCCGGCCGTGACGAAGTGCTGCTGCCGCTGATGCGCCAGGTCGCCGACGCCATCCGCGCCCTGGCCCACGCCCACGCCGAAGTGCCAATGCTGTCGCGCACCCACGGCCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCCAACGTCGTGTACCGCCTGGAGCGCCAGATCGCCCAGGTT  >  NC_002947/4527262‑4527548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑