Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,673,550 G→C 8.8% G108R (GGG→CGG) ‡ PP_4137 → outer membrane siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,673,5500GC 8.8% 213.9 / 8.6 68G108R (GGG→CGG) ‡PP_4137outer membrane siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (40/22);  new base C (3/3);  total (43/25)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

TGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGG  >  NC_002947/4673407‑4673689
                                                                                                                                               |                                                                                                                                           
tggtggACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGgcg                                                                                                                                        >  5:53581/1‑149 (MQ=255)
  gtggACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGgcg                                                                                                                                        <  3:13702/147‑1 (MQ=255)
     gACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACgg                                                                                                                                   <  7:129703/149‑1 (MQ=255)
     gACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCACgg                                                                                                                                   <  1:7026/149‑1 (MQ=255)
        aTGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTc                                                                                                                                <  2:218110/149‑1 (MQ=255)
             gcgcGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGgtg                                                                                                                           >  3:251214/1‑149 (MQ=255)
              cgcgTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTTAACCGTGCGCCGGGGGCGCACGGTTCCGgtgt                                                                                                                          >  4:63403/1‑149 (MQ=255)
              cgcgTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCACGGtt                                                                                                                                 <  3:183549/142‑1 (MQ=255)
              cgcgTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCACGGtt                                                                                                                                 >  4:183549/1‑142 (MQ=255)
                      gCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACAcc                                                                                                                   >  4:108096/1‑148 (MQ=255)
                      gCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACAcc                                                                                                                   <  3:108096/148‑1 (MQ=255)
                      gCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCa                                                                                                                  >  3:58104/1‑149 (MQ=255)
                      gCAAGGCCGAGTGGCCAGCAACCAGGACGAGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCa                                                                                                                  >  7:216213/1‑149 (MQ=255)
                           gCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTc                                                                                                             >  1:127997/1‑149 (MQ=255)
                            ccGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTc                                                                                                             >  6:152688/1‑148 (MQ=255)
                                 tGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGTCCTCAACCGTGCGGCGGGGGAGCACGGTTACGGTGTCTACACCATTTTCGACGGc                                                                                                       >  3:6535/1‑149 (MQ=255)
                                 tGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCACgg                                                                                                                                   <  7:61198/121‑1 (MQ=255)
                                 tGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCACgg                                                                                                                                   >  8:61198/1‑121 (MQ=255)
                                    ccAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCCGTGTCTAACCCATGTTCGACGGCCTg                                                                                                    >  2:325883/1‑149 (MQ=255)
                                     cAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGc                                                                                                   >  2:168160/1‑149 (MQ=255)
                                     cAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCAGGGGGCGCACGGTTCCGGTGTCTACACCATGTTAGACGGACTGc                                                                                                   >  8:150989/1‑149 (MQ=255)
                                         aaCCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGtt                                                                                               <  5:19761/149‑1 (MQ=255)
                                                 cGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGcc                                                                                       >  8:240031/1‑149 (MQ=255)
                                                            aCCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGCCCGGCCCGGGCGGCCCg                                                                            >  8:305150/1‑149 (MQ=255)
                                                            aCCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGAcc                                                                                           <  3:142475/134‑1 (MQ=255)
                                                            aCCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGAcc                                                                                           >  4:142475/1‑134 (MQ=255)
                                                              cagccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGa                                                                                             <  3:216208/130‑1 (MQ=255)
                                                              cagccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGa                                                                                             >  4:216208/1‑130 (MQ=255)
                                                                gccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGggc                                                                                  >  5:159876/1‑139 (MQ=255)
                                                                gccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGggc                                                                                  <  6:159876/139‑1 (MQ=255)
                                                                 ccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTTAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTa                                                                       <  3:63403/149‑1 (MQ=255)
                                                                  cagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTAc                                                                      >  8:267620/1‑149 (MQ=255)
                                                                   agGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGcc                                                                                                  >  7:248105/1‑120 (MQ=255)
                                                                   agGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGcc                                                                                                  <  8:248105/120‑1 (MQ=255)
                                                                       gtgtTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCTAGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACt                                                                 >  4:31102/1‑149 (MQ=255)
                                                                            cGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTc                                                             <  7:150989/148‑1 (MQ=255)
                                                                              cccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                   >  4:268528/1‑140 (MQ=255)
                                                                              cccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGaa                                                                   <  3:268528/140‑1 (MQ=255)
                                                                              cccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGaa                                                          >  8:138697/1‑149 (MQ=255)
                                                                               ccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAAc                                                         >  7:220859/1‑149 (MQ=255)
                                                                                cAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAAcc                                                        >  4:132183/1‑149 (MQ=255)
                                                                                             aCGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGc                                           >  6:111990/1‑149 (MQ=255)
                                                                                               gATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCg                                         >  8:278755/1‑149 (MQ=255)
                                                                                                    cATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGcc                                     >  2:46054/1‑148 (MQ=255)
                                                                                                    cATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCg                                    <  7:267620/149‑1 (MQ=255)
                                                                                                    cATCAAGCTGTCGATACGCGGATCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTAGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGcc                                     >  7:21918/1‑148 (MQ=255)
                                                                                                     aTCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCg                                    >  6:140340/1‑148 (MQ=255)
                                                                                                      tCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCg                                    >  6:85385/1‑147 (MQ=255)
                                                                                                        aaGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAg                                 <  6:79051/148‑1 (MQ=255)
                                                                                                              tCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACCGTTACGGTGTGTACACCATGTTAGACGGCCAGCCCTTTACCGGCCCCGGCGGCACGCCTTACGAACTTTTAGAACCGCAGTGGGGGAGCCGTTCCGAAGTGCTGCg                          >  2:163398/1‑149 (MQ=255)
                                                                                                                gATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTg                        <  7:240031/149‑1 (MQ=255)
                                                                                                                gATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACCGCCTGCCGATGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCAGCGTg                        >  1:321868/1‑149 (MQ=255)
                                                                                                                  tACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGgc                      >  6:21301/1‑149 (MQ=255)
                                                                                                                  tACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGgc                      >  2:9927/1‑149 (MQ=255)
                                                                                                                    ggcggcgCGGGCCCCACCCGGGCGCCGGGGGCCCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGgcgc                    <  1:325883/148‑1 (MQ=255)
                                                                                                                    cgcgGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGgcgc                    <  3:132183/149‑1 (MQ=255)
                                                                                                                     gcgGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGgcgc                    >  7:186189/1‑148 (MQ=255)
                                                                                                                        gCTCGGGCCTCACCGGGGCGCGGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAAt                <  2:321868/149‑1 (MQ=255)
                                                                                                                         cTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATg               <  8:186189/149‑1 (MQ=255)
                                                                                                                         cTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATg               >  3:107823/1‑149 (MQ=255)
                                                                                                                             ggCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGGtt           >  8:292858/1‑149 (MQ=255)
                                                                                                                              gCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACTAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATCGCttt          <  7:292858/149‑1 (MQ=255)
                                                                                                                              gCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCttt          <  8:216213/149‑1 (MQ=255)
                                                                                                                                cTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGa        >  4:312221/1‑149 (MQ=255)
                                                                                                                                  cAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGAc       >  6:77387/1‑148 (MQ=255)
                                                                                                                                     ccGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAg   >  3:304627/1‑149 (MQ=255)
                                                                                                                                      cGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAgg  <  1:9927/149‑1 (MQ=255)
                                                                                                                                      cGTGCGCCCCCGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAgg  >  3:120917/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                           
TGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCACGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGG  >  NC_002947/4673407‑4673689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑