Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,705,413	T→A	11.2%	intergenic (‑151/+26)	kdpF ← / ← PP_4162	potassium‑transporting ATPase subunit F/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,705,413	0	T	A	11.2%	109.8 / 4.2	43	intergenic (‑151/+26)	kdpF/PP_4162	potassium‑transporting ATPase subunit F/membrane protein
Reads supporting (aligned to +/- strand):  ref base T (21/17);  new base A (3/2);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

GTGGATCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGC  >  NC_002947/4705265‑4705557                                                                                                                                                     |                                                                                                                                                  gtgGATCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCt                                                                                                                                                   >  1:16837/1‑149 (MQ=255)  tgGATCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATTAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCtt                                                                                                                                                  >  2:56257/1‑149 (MQ=255)                ttACGGCTTGGGCCGCTTTGGGCAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGAGTAAAAATCAGCGTTGTGGTCTGACTGTAACGGCCTATTCGCGGGGTTGCCCGCGAAAGGGc                                                                                                                                    >  7:201762/1‑149 (MQ=255)                                    gggAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATtgttgt                                                                                                                >  2:99280/1‑149 (MQ=255)                                    gggAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATtgttgt                                                                                                                >  7:1985/1‑149 (MQ=255)                                       aaaTTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTggc                                                                                                             >  5:251599/1‑149 (MQ=255)                                                   ggAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAAAGGACTCTTCGCGGGCTTGCCGGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGcgcc                                                                                                 >  8:95196/1‑149 (MQ=255)                                                           gTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTAc                                                                                                                              >  3:86363/1‑112 (MQ=255)                                                             aaaGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTTCAGGCTTATTGTTGTGGCGGCTGCTGCCCCGCGTTACGGc                                                                                       >  8:39002/1‑149 (MQ=255)                                                                  ggCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccag                                                                                  >  3:130934/1‑149 (MQ=255)                                                                            gggggtgggggtGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCcagcag                                                                          >  8:300277/1‑147 (MQ=255)                                                                            gggggtgggggtGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCcagcag                                                                          >  6:166788/1‑147 (MQ=255)                                                                                   ggggtgggTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTa                                                                 <  4:130934/149‑1 (MQ=255)                                                                                         ggtATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTAc                                                           <  6:251599/149‑1 (MQ=255)                                                                                            ataAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGc                                                        >  3:195269/1‑149 (MQ=255)                                                                                             taAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGc                                                        <  4:195269/148‑1 (MQ=255)                                                                                             taAAGAATGCGTAAAAATCAGCCGTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGTAGGCTACAGCCtgctgct                                                       <  1:56257/149‑7 (MQ=39)                                                                                              aaaGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccagtccag                                                                             >  6:178038/1‑126 (MQ=255)                                                                                              aaaGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccagtccag                                                                             <  5:178038/126‑1 (MQ=255)                                                                                                 gAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCa                                                  <  7:39002/149‑1 (MQ=255)                                                                                                          aaaaTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGc                                          <  7:300277/148‑1 (MQ=255)                                                                                                           aaaTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTAc                                                           >  2:211880/1‑131 (MQ=255)                                                                                                           aaaTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTAc                                                           <  1:211880/131‑1 (MQ=255)                                                                                                             aTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTAc                                                                                          <  7:8681/98‑1 (MQ=255)                                                                                                             aTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTAc                                                                                          >  8:8681/1‑98 (MQ=255)                                                                                                                    ttGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAg                               <  1:99280/149‑1 (MQ=255)                                                                                                                    ttGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAg                               <  5:166788/149‑1 (MQ=255)                                                                                                                     tgtgGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACg                                                                                         >  5:330585/1‑91 (MQ=255)                                                                                                                     tgtgGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACg                                                                                         <  6:330585/91‑1 (MQ=255)                                                                                                                      gtgGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAgg                              <  2:16837/148‑1 (MQ=255)                                                                                                                               cTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGaa                      >  4:48993/1‑147 (MQ=255)                                                                                                                               cTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGaa                      <  3:48993/147‑1 (MQ=255)                                                                                                                                    aCGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAg               >  3:237451/1‑149 (MQ=255)                                                                                                                                      ggCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAACAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAgg              >  4:87672/1‑148 (MQ=255)                                                                                                                                       gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgc             >  4:269932/1‑148 (MQ=255)                                                                                                                                        ccTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgc             >  2:67424/1‑147 (MQ=255)                                                                                                                                         ctctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCTCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaa           <  1:67424/149‑1 (MQ=255)                                                                                                                                          ctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAACAGCAAGCAGAACCCAACCGC‑CAGCAAGGACGGCCCGATGAAAAGCCCAAACAACCCAAAGccaaa          >  7:48507/3‑147 (MQ=25)                                                                                                                                            ttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTa                                                                 >  7:183096/1‑92 (MQ=37)                                                                                                                                            ttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTa                                                                 <  8:183096/92‑1 (MQ=37)                                                                                                                                            ttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGa                       <  5:254811/133‑1 (MQ=255)                                                                                                                                            ttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGa                       >  6:254811/1‑133 (MQ=255)                                                                                                                                               gcgGGCTTGCCCGCTAAAGGGCCGTTACAGGCTTATTGTTGTGTCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCaagcaagc     <  7:264506/148‑1 (MQ=255)                                                                                                                                                cgGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAgccg   >  2:69617/1‑149 (MQ=255)                                                                                                                                                 gggCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAgccgc  <  8:1985/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GTGGATCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGC  >  NC_002947/4705265‑4705557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑