Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,705,424	A→G	11.1%	intergenic (‑162/+15)	kdpF ← / ← PP_4162	potassium‑transporting ATPase subunit F/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,705,424	0	A	G	11.1%	112.5 / 5.5	45	intergenic (‑162/+15)	kdpF/PP_4162	potassium‑transporting ATPase subunit F/membrane protein
Reads supporting (aligned to +/- strand):  ref base A (22/18);  new base G (3/2);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

TTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACA  >  NC_002947/4705280‑4705563                                                                                                                                                 |                                                                                                                                             ttACGGCTTGGGCCGCTTTGGGCAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGAGTAAAAATCAGCGTTGTGGTCTGACTGTAACGGCCTATTCGCGGGGTTGCCCGCGAAAGGGc                                                                                                                                          >  7:201762/1‑149 (MQ=255)                     gggAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATtgttgt                                                                                                                      >  7:1985/1‑149 (MQ=255)                     gggAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATtgttgt                                                                                                                      >  2:99280/1‑149 (MQ=255)                        aaaTTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTggc                                                                                                                   >  5:251599/1‑149 (MQ=255)                                    ggAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAAAGGACTCTTCGCGGGCTTGCCGGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGcgcc                                                                                                       >  8:95196/1‑149 (MQ=255)                                            gTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTAc                                                                                                                                    >  3:86363/1‑112 (MQ=255)                                              aaaGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTTCAGGCTTATTGTTGTGGCGGCTGCTGCCCCGCGTTACGGc                                                                                             >  8:39002/1‑149 (MQ=255)                                                   ggCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccag                                                                                        >  3:130934/1‑149 (MQ=255)                                                             gggggtgggggtGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCcagcag                                                                                >  8:300277/1‑147 (MQ=255)                                                             gggggtgggggtGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCcagcag                                                                                >  6:166788/1‑147 (MQ=255)                                                                    ggggtgggTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTa                                                                       <  4:130934/149‑1 (MQ=255)                                                                          ggtATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTAc                                                                 <  6:251599/149‑1 (MQ=255)                                                                             ataAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGc                                                              >  3:195269/1‑149 (MQ=255)                                                                              taAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGc                                                              <  4:195269/148‑1 (MQ=255)                                                                              taAAGAATGCGTAAAAATCAGCCGTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGTAGGCTACAGCCtgctgct                                                             <  1:56257/149‑7 (MQ=39)                                                                               aaaGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccagtccag                                                                                   >  6:178038/1‑126 (MQ=255)                                                                               aaaGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccagtccag                                                                                   <  5:178038/126‑1 (MQ=255)                                                                                  gAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCa                                                        <  7:39002/149‑1 (MQ=255)                                                                                           aaaaTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGc                                                <  7:300277/148‑1 (MQ=255)                                                                                            aaaTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTAc                                                                 >  2:211880/1‑131 (MQ=255)                                                                                            aaaTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTAc                                                                 <  1:211880/131‑1 (MQ=255)                                                                                              aTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTAc                                                                                                >  8:8681/1‑98 (MQ=255)                                                                                              aTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTAc                                                                                                <  7:8681/98‑1 (MQ=255)                                                                                                     ttGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAg                                     <  5:166788/149‑1 (MQ=255)                                                                                                     ttGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAg                                     <  1:99280/149‑1 (MQ=255)                                                                                                      tgtgGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACg                                                                                               >  5:330585/1‑91 (MQ=255)                                                                                                      tgtgGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACg                                                                                               <  6:330585/91‑1 (MQ=255)                                                                                                       gtgGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAgg                                    <  2:16837/148‑1 (MQ=255)                                                                                                                cTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGaa                            >  4:48993/1‑147 (MQ=255)                                                                                                                cTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGaa                            <  3:48993/147‑1 (MQ=255)                                                                                                                     aCGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAg                     >  3:237451/1‑149 (MQ=255)                                                                                                                       ggCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAACAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAgg                    >  4:87672/1‑148 (MQ=255)                                                                                                                        gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgc                   >  4:269932/1‑148 (MQ=255)                                                                                                                         ccTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgc                   >  2:67424/1‑147 (MQ=255)                                                                                                                          ctctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCTCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaa                 <  1:67424/149‑1 (MQ=255)                                                                                                                           ctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAACAGCAAGCAGAACCCAACCGC‑CAGCAAGGACGGCCCGATGAAAAGCCCAAACAACCCAAAGccaaa                >  7:48507/3‑147 (MQ=25)                                                                                                                             ttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTa                                                                       >  7:183096/1‑92 (MQ=37)                                                                                                                             ttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTa                                                                       <  8:183096/92‑1 (MQ=37)                                                                                                                             ttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGa                             >  6:254811/1‑133 (MQ=255)                                                                                                                             ttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGa                             <  5:254811/133‑1 (MQ=255)                                                                                                                                gcgGGCTTGCCCGCTAAAGGGCCGTTACAGGCTTATTGTTGTGTCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCaagcaagc           <  7:264506/148‑1 (MQ=255)                                                                                                                                 cgGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAgccg         >  2:69617/1‑149 (MQ=255)                                                                                                                                  gggCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAgccgc        <  8:1985/149‑1 (MQ=255)                                                                                                                                    cagagCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAgccgcc       <  6:109598/144‑1 (MQ=255)                                                                                                                                        gCCCGCGAAAGGGCCGTTACAGGCTTATTGTAGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAaca  >  1:33608/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                             TTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGC‑CAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACA  >  NC_002947/4705280‑4705563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑