Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,735,320 C→A 100% M105I (ATG→ATT)  sucA ← 2‑oxoglutarate dehydrogenase subunit E1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,735,3200CA100.0% 175.5 / NA 53M105I (ATG→ATT) sucA2‑oxoglutarate dehydrogenase subunit E1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/29);  total (24/29)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTCTTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGCATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGA  >  NC_002947/4735176‑4735454
                                                                                                                                                |                                                                                                                                      
ctctTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATAc                                                                                                                                    >  4:358/1‑149 (MQ=255)
   tttGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGa                                                                                                                                  >  1:307726/1‑148 (MQ=255)
   tttGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGa                                                                                                                                  >  8:192167/1‑148 (MQ=255)
   tttGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGa                                                                                                                                  >  4:219890/1‑148 (MQ=255)
     tGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGa                                                                                                                                  <  6:175954/146‑1 (MQ=255)
     tGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATa                                                                                                                                <  3:21446/148‑1 (MQ=255)
          aTGAACAGGTCGCCGGCACGGAAGGTCGTATCGAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTg                                                                                                                          >  1:160520/1‑149 (MQ=255)
                aGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAg                                                                                                                    >  5:320464/1‑149 (MQ=255)
                  gTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTc                                                                                                                  <  8:168408/149‑1 (MQ=255)
                   tCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCg                                                                                                                 >  5:299575/1‑149 (MQ=255)
                       cGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAg                                                                                                                               >  8:73984/1‑131 (MQ=255)
                       cGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAg                                                                                                                               <  7:73984/131‑1 (MQ=255)
                        ggCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGaa                                                                                                            <  4:16508/149‑1 (MQ=255)
                            cGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTc                                                                                                        <  6:29920/149‑1 (MQ=255)
                                        tCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGcttctt                                                                                            >  6:24469/1‑149 (MQ=255)
                                        tCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGcttctt                                                                                            >  6:34600/1‑149 (MQ=255)
                                          aaGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCttcttc                                                                                           <  7:192167/148‑1 (MQ=255)
                                           aGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTtctc                                                                                         <  6:299575/149‑1 (MQ=255)
                                              tCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGc                                                                                                 <  6:32987/138‑1 (MQ=255)
                                              tCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGc                                                                                                 >  5:32987/1‑138 (MQ=255)
                                                  cATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTTCGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGATTACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCa                                                                                  <  6:14368/149‑1 (MQ=255)
                                                   aTTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGAGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCAc                                                                                 >  3:175922/1‑149 (MQ=255)
                                                      ggTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTg                                                                               <  5:194596/148‑1 (MQ=255)
                                                       gTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGc                                                                              <  3:219890/148‑1 (MQ=255)
                                                         cAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCa                                                                           >  5:162427/1‑149 (MQ=255)
                                                          aaGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCAc                                                                          >  7:93836/1‑149 (MQ=255)
                                                              cGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATTAGTCGCAGAACTTTAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTc                                                                      >  2:319775/1‑149 (MQ=255)
                                                               atAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTCGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTcc                                                                     >  7:204843/2‑149 (MQ=255)
                                                                 aGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCcg                                                                   >  6:43691/1‑149 (MQ=255)
                                                                  gTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCAACAACCCCAACGGGTCGAGCTTGGCATCATGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTAAACCTCCTTCTTCTCCTGTTCACTGCTCACGCTccctg                                                                  >  1:229901/1‑147 (MQ=255)
                                                                     attgatCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCa                                                                                                                     <  8:268121/95‑1 (MQ=255)
                                                                     attgatCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCa                                                                                                                     >  7:268121/1‑95 (MQ=255)
                                                                      cgatctgACAGGTGTACGGGCGCAGGTGGCTGCCACAACCCCTACGGGTCGAGCTTTGCAGGCTGATGGCCGCGAATACGATAGGCCTGGATGAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGa                                                              <  6:64746/143‑1 (MQ=255)
                                                                            gACAGGGCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCg                                                         <  8:121982/148‑1 (MQ=255)
                                                                             aCAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCgt                                                                                       <  1:94369/117‑1 (MQ=255)
                                                                             aCAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCgt                                                                                       >  2:94369/1‑117 (MQ=255)
                                                                             aCAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGaaagaacg                                                       >  5:64746/1‑144 (MQ=255)
                                                                             aCAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGt                                                       <  8:204843/149‑1 (MQ=255)
                                                                               aGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTg                                                     >  3:236027/1‑149 (MQ=255)
                                                                                ggTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTgg                                                    <  6:162427/149‑1 (MQ=255)
                                                                                      cGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGggcgc                                                <  4:175922/147‑1 (MQ=255)
                                                                                         gcgcAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGggcgcggcgc                                           >  3:187366/1‑149 (MQ=255)
                                                                                          cgcAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCt                                          <  5:43691/149‑1 (MQ=255)
                                                                                          cgcAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCt                                          >  6:132945/1‑149 (MQ=255)
                                                                                           gcAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATTAGTCGCAGAACTTTAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTg                                         <  1:319775/149‑1 (MQ=255)
                                                                                                 cgcTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGAGCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCtt                                   <  4:208752/149‑1 (MQ=255)
                                                                                                  gcTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCttt                                  <  5:24469/149‑1 (MQ=255)
                                                                                                      ccACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGc                                <  3:358/147‑1 (MQ=255)
                                                                                                       cacaACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCcag                             <  4:187366/149‑1 (MQ=255)
                                                                                                       cacaACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCcag                             <  2:307726/149‑1 (MQ=255)
                                                                                                                         aGCTTGGCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTcgcg           <  2:160520/149‑1 (MQ=255)
                                                                                                                              ggCAGCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGAtt       <  6:320464/148‑1 (MQ=255)
                                                                                                                                  gCCTGATGGCCGCGAATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGa  <  8:93836/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                      
CTCTTTGCCGATGAACAGGTCGCCGGCACGGAAGGTCGTATCAAGATCGGCATTGGTCAAGCCGTAGTGATTGATCGACAGGTCTACGGGCGCAGGGCGCTGCCACAACCCCAACGGGTCGAGCTTGGCAGCCTGATGGCCGCGCATACGATAGGCCTGGATCAGTCGCAGAACTTCAACCTGCTTCTTCTCGTGTTCACTGCTCACGCTCCCGGCGGAAACCGGTTGGGCGCGGCGCTGGTTCTTTGCCAGCAGTACGAAATGGTCGCGGATTGTCGA  >  NC_002947/4735176‑4735454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑