Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,796,604 A→G 12.7% intergenic (‑162/+56) pvdH ← / ← PP_4224 diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,796,6040AG12.7% 109.8 / 8.1 47intergenic (‑162/+56)pvdH/PP_4224diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (21/20);  new base G (3/3);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

GCAAGGCTGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAC  >  NC_002947/4796462‑4796749
                                                                                                                                              |                                                                                                                                                 
gCAAGGCTGGAAGCGGGGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAAcccg                                                                                                                                             <  5:280818/149‑1 (MQ=255)
       tGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCATGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAg                                                                                                                                      >  5:262113/1‑149 (MQ=255)
           aGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGc                                                                                                                                  <  3:313128/149‑1 (MQ=255)
             cGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCATGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCgg                                                                                                                                <  6:262113/149‑1 (MQ=255)
              ggTGGACATGGGTAAATCCTCGCTAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCTATTTTATTTTTGTCTGTGCTTGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCggg                                                                                                                               <  5:171370/149‑1 (MQ=255)
              ggTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCggg                                                                                                                               <  7:287138/149‑1 (MQ=255)
                     aTGGGTAAATCGACGCGAGCAAGCGGATGGGCGGTAATCGCTTTGGAAACGCTTGTGTGGGGGGAGGATTTAGCGGTTGATACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCTTGTGAACCCGCTCCTACGGGCGGGTTTAccc                                                                                                                        <  5:292825/149‑1 (MQ=25)
                     aTGGGTAAATCCTGGCGTGCAAGCGTTTGGGCTGTTATCGCTTTGGAAAGGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAccc                                                                                                                        <  7:51408/149‑1 (MQ=25)
                     aTGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTGCGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAccc                                                                                                                        >  6:37700/1‑149 (MQ=255)
                     aTGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAccc                                                                                                                        <  2:260611/149‑1 (MQ=38)
                     aTGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAccc                                                                                                                        <  2:37562/149‑1 (MQ=255)
                          tAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCTGGTTCACCcgcg                                                                                                                     >  3:72597/1‑147 (MQ=255)
                          tAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgcg                                                                                                                     >  8:147323/1‑147 (MQ=255)
                                         aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGc                                                                                                    >  4:287950/1‑149 (MQ=255)
                                               aTGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTGCGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATaaa                                                                                              <  5:37700/149‑1 (MQ=255)
                                                   gCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACAt                                                                                           >  1:689/1‑148 (MQ=255)
                                                          tCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGcc                                                                                    >  8:14223/1‑148 (MQ=255)
                                                           cGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAgg                                                                                                     >  7:52388/1‑130 (MQ=255)
                                                           cGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAgg                                                                                                     <  8:52388/130‑1 (MQ=255)
                                                            gCTTTGGAAACGCTTGAGTGGGGGTAGGAGTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGTGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCa                                                                                  <  3:187164/148‑1 (MQ=255)
                                                            gCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAg                                                                                 <  5:305777/149‑1 (MQ=255)
                                                              tttGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGa                                                                               >  8:40271/1‑149 (MQ=255)
                                                                   aaaCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACgcgc                                                                          >  6:182862/1‑149 (MQ=255)
                                                                   aaaCGCTTGAGAGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGATCACCCGCCAAGACGCCACCACAGGCCATAAACATCAAATCCCATGaagagc                                                                          >  8:78934/1‑144 (MQ=37)
                                                                      cGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCgg                                                                       <  7:147323/149‑1 (MQ=255)
                                                                               ggggggAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTg                                                                 >  7:211069/1‑146 (MQ=255)
                                                                                   ggaggaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACACGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCTCCGGTACCTGcaacaa                                                           >  5:295002/1‑148 (MQ=255)
                                                                                       gaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCACTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAgg                                                                                                     >  3:175262/1‑102 (MQ=255)
                                                                                       gaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCACTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAgg                                                                                                     <  4:175262/102‑1 (MQ=255)
                                                                                         tttAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGc                                                    <  7:14223/149‑1 (MQ=255)
                                                                                                        gcgATTTTATTTTTGTCTGTGCTGGCCTCTTCCCCGGTAAAACCGCCCGTAAGAGCGGGTTAACCCGCGAAGAGGCCAGCCCAGGCGATAAAAAAAAAATCCCAGGACGCCCCGGTACCAGCAACAAAACCCCCAACGCCTAACTACgg                                     >  6:70991/1‑149 (MQ=255)
                                                                                                            ttttattttTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGt                                 <  5:70991/149‑1 (MQ=255)
                                                                                                                 tttttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa                             <  5:182862/148‑1 (MQ=255)
                                                                                                                 tttttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa                             <  7:78934/148‑1 (MQ=255)
                                                                                                                 tttttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa                             <  7:40271/148‑1 (MQ=255)
                                                                                                                   tttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAgc                           <  3:287950/148‑1 (MQ=255)
                                                                                                                       tCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAgg                      <  6:295002/149‑1 (MQ=255)
                                                                                                                             cTGGCCTGTTCGCTTGTAAAACCGCGCGTAGGTGCGGGTTCAGCCTCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCGTGGAGGCGCGGGTACCTGCAACATAACCCGCAAGCCGTCACTACGGCTGTCAAATCGCAGGCTAcc                 <  4:72597/148‑1 (MQ=255)
                                                                                                                               ggCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACCCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCgg               >  8:30358/1‑148 (MQ=255)
                                                                                                                                     ttCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCg        >  3:27086/1‑149 (MQ=255)
                                                                                                                                        gcgGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTg     >  5:117718/1‑149 (MQ=37)
                                                                                                                                         cgGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTg     >  3:194168/1‑148 (MQ=255)
                                                                                                                                          gggTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCa   >  4:23943/1‑149 (MQ=38)
                                                                                                                                          gggTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACACGCATGCCGTCACTACGGCTGTCAACGCGCAGGCTCCCGGCACACCGCTGcc   >  8:235356/1‑148 (MQ=255)
                                                                                                                                           ggTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGGCGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc  >  3:1013/1‑149 (MQ=37)
                                                                                                                                            gTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc  >  8:208017/1‑148 (MQ=255)
                                                                                                                                            gTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc  >  1:314814/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GCAAGGCTGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAC  >  NC_002947/4796462‑4796749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑