| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,832,071 | T→G | 7.4% | intergenic (+46/+106) | pvdS → / ← pvdY | alternative sigma factor/hydroxyproline acetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,832,071 | 0 | T | G | 7.4% | 164.6 / 4.7 | 54 | intergenic (+46/+106) | pvdS/pvdY | alternative sigma factor/hydroxyproline acetylase |
| Reads supporting (aligned to +/- strand): ref base T (25/25); new base G (2/2); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
AGGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGAAAAACCGCTCCCGCCCCAG > NC_002947/4831925‑4832218 | aGGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTTCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCtt < 1:148688/148‑1 (MQ=255) gACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGATCTACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCtttt < 1:230943/149‑1 (MQ=255) aTTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTttgctt > 1:289395/1‑149 (MQ=255) ttGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCAAGAGCGAACCTGGATCGCTCTCGCTTttgcttg < 6:128509/149‑1 (MQ=255) aGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAgcgc < 7:156054/149‑1 (MQ=255) tGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTAt > 4:39668/1‑149 (MQ=255) ggCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAgcg > 4:45680/1‑140 (MQ=255) ggCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAgcg < 3:45680/140‑1 (MQ=255) tCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGtttt > 5:74027/1‑149 (MQ=255) gACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCa < 7:19990/126‑1 (MQ=255) gACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GAAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCa > 8:19990/1‑126 (MQ=255) ccTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGc > 8:89785/1‑144 (MQ=255) ccTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGc < 7:89785/144‑1 (MQ=255) cTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATAc > 6:39725/1‑149 (MQ=255) gTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGc < 6:137011/148‑1 (MQ=255) aaCTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCaa < 3:169541/148‑1 (MQ=255) ttCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAAc > 3:74260/1‑149 (MQ=255) tCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACt > 2:59398/1‑149 (MQ=255) cgcgATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAATGCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAgcg > 1:256486/1‑149 (MQ=255) gTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAAcgccg > 1:283542/1‑148 (MQ=255) gTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAAcgccg > 4:73615/1‑148 (MQ=255) tGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAAcgccgc > 6:222676/1‑148 (MQ=255) gCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTAt > 8:221352/1‑149 (MQ=255) ccGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATg < 5:328225/149‑1 (MQ=255) ccGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATg < 5:222676/149‑1 (MQ=255) cAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTc > 3:212789/1‑149 (MQ=255) aGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTtgtg < 2:289395/149‑1 (MQ=255) ccAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAaca > 3:243830/1‑149 (MQ=255) ccAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAaca < 5:39725/149‑1 (MQ=255) cAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAacac < 2:256486/149‑1 (MQ=255) ggCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAAcacca < 5:112473/149‑1 (MQ=255) gCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAAcaccac < 7:167887/149‑1 (MQ=255) gCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAAcacca > 2:313951/1‑148 (MQ=255) aaGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGGCGTTATTTGTGAAACACCACACTa < 3:313531/148‑1 (MQ=255) gAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGcc < 4:243830/149‑1 (MQ=255) gAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGcc > 5:131002/1‑149 (MQ=255) gAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGcc > 4:116368/1‑149 (MQ=255) tagcgcTGCAA‑GCAAAAGCGAGAGCGATCCAGGTTCGCTCTGGCGTTTGCTTGCAGTGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCTTAAGTTATGGTCGTTATTTGTGa < 7:201918/129‑1 (MQ=21) tagcgcTGCAA‑GCAAAAGCGAGAGCGATCCAGGTTCGCTCTGGCGTTTGCTTGCAGTGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTTTTTGTGa > 8:201918/6‑134 (MQ=21) gACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGcccc > 3:258920/1‑148 (MQ=255) cTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTAc < 3:39668/149‑1 (MQ=255) caa‑gcaaACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTtgt < 3:201696/124‑1 (MQ=255) caa‑gcaaACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTtgt > 4:201696/1‑124 (MQ=255) aa‑gcaaACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGcccc < 2:202745/142‑1 (MQ=255) aa‑gcaaACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGcccc > 1:202745/1‑142 (MQ=255) aa‑gcaaACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCa < 4:258920/149‑1 (MQ=255) aa‑gcaaAAGCGAGAGCGATCCAGGTTCGCTCTGGCGTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGt > 4:255042/1‑147 (MQ=38) caaACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCTCAGCGCAAAAAACGCAGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCACCTACGTCAGCt > 4:180240/1‑149 (MQ=255) cAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGcc > 6:286956/1‑130 (MQ=255) cAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGcc < 5:286956/130‑1 (MQ=255) gagCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGAt > 5:177999/1‑149 (MQ=255) aaCCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGaaa < 1:150815/149‑1 (MQ=255) ccTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGaaaaa < 1:59398/149‑1 (MQ=255) caggttCGCTCTGGCGTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGAAAAAc < 6:233161/144‑1 (MQ=255) gCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGAAAAACCGCTCCCGcccc < 6:74027/148‑1 (MQ=255) gCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGAAAAACCGCTCCCGccc < 7:221352/147‑1 (MQ=255) ttttGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGAAAAACCGCTCCCGCCCCAg > 2:161561/1‑148 (MQ=255) | AGGACATTGCCAAGGAACTGGGCGTATCGCCGACCTTGGTCAACTTCATGATCCGCGATGCGCTGGTGCATTGCCGCAAGACGGCCAGTCGCCAGGCCTGAAGAT‑GACTGCAA‑GCAAACGCCAGAGCGAACCTGGATCGCTCTCGCTTTTGCTTGCAGCGCTAGTATGGTGTTTTGCAAATACGGCCAATAACTCGCAGCGCAAAAAACGCCGCAAGTTATGGTCGTTATTTGTGAAACACCACACTAGCCCCTACGTCAGCTTGCAACGATCGAAAAACCGCTCCCGCCCCAG > NC_002947/4831925‑4832218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |