Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,837,281 A→G 6.4% S430G (AGC→GGC)  ccoN‑I → cbb3‑type cytochrome c oxidase subunit 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,837,2810AG 6.4% 177.3 / 2.6 62S430G (AGC→GGC) ccoN‑Icbb3‑type cytochrome c oxidase subunit 1
Reads supporting (aligned to +/- strand):  ref base A (33/25);  new base G (2/2);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

TGCGCACTTCTGGCTGGCCACCATCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCTTGAGGAGAAAC  >  NC_002947/4837139‑4837426
                                                                                                                                              |                                                                                                                                                  
tGCGGACTTCTGGCTGGCCACCATCGGTACCGTGCTGTACATCGCCTCGATGTTGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCTTTCGTGGAAACCTTGGTGGCCAGCCAcc                                                                                                                                              <  8:65906/149‑1 (MQ=255)
 gcgcACTTCTGGCTGGCCACCATCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCAcc                                                                                                                                              >  7:29245/1‑148 (MQ=255)
   gcACTTCTGGCTGGCCACCATCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCTGGCCACCAAg                                                                                                                                           <  1:1507/149‑1 (MQ=255)
       ttCTGGCTGGCCACCATCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCAc                                                                                                                                               <  2:77682/141‑1 (MQ=255)
       ttCTGGCTGGCCACCATCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCAc                                                                                                                                               >  1:77682/1‑141 (MQ=255)
         ctggctggCCACCATCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTtc                                                                                                                                      <  1:308066/148‑1 (MQ=255)
              tggcCACCATCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTg                                                                                                                                >  4:187505/1‑149 (MQ=255)
                  caccaTCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCt                                                                                                                            >  2:237878/1‑149 (MQ=255)
                    ccaTCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTtc                                                                                                                          <  7:103743/149‑1 (MQ=255)
                     caTCGGTACCGTGCTGTACATCGCCTAGATGTGGGTCAACTGCATCGCACAGGGCCTGATTTGTCGCGCAGTCAACAGCGACTGCACGCTCACCTAATCGTTCGTGGAAACCTTGGTGTCCAGCCACCCAGGCTTCGTCGTGCGCTtcg                                                                                                                         <  1:293152/149‑1 (MQ=255)
                                     tACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGCCGGCACGCTCACCTACTCGTTCGTCGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCt                                                                                                         >  2:304496/1‑148 (MQ=255)
                                       cATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTc                                                                                                       <  2:126392/148‑1 (MQ=255)
                                          cGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCt                                                                                                     >  5:182696/1‑147 (MQ=255)
                                             cTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCg                                                                                                <  6:107333/149‑1 (MQ=255)
                                             cTCGATGTGGGTCAACGGCATCGCCCAGCGCCTGATGTGGCGCGCGGTCACCAGCGAAGGACCGAGCACCTACACGTTCGTGGAAACCTTGGTGGCCAGCCACACAGGCTTCGTCGTGCGCTTCGTC‑GGCGTCGCCCTCTTCCTCcgcg                                                                                                >  3:272702/1‑149 (MQ=25)
                                                   gtgGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGt                                                                                          >  8:227820/1‑149 (MQ=255)
                                                     gggTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTc                                                                                        >  6:228324/1‑149 (MQ=255)
                                                      ggTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTcc                                                                                       >  6:187635/1‑149 (MQ=255)
                                                               cATCGCCCAGGGTCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCt                                                                              >  5:5437/1‑149 (MQ=255)
                                                                 tCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATg                                                                                  >  7:233839/1‑143 (MQ=255)
                                                                 tCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATg                                                                                  <  8:233839/143‑1 (MQ=255)
                                                                   gCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGaa                                                                          >  6:65172/1‑149 (MQ=255)
                                                                   gCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGa                                                                           <  6:182696/148‑1 (MQ=255)
                                                                    cccAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAac                                                                         <  7:227820/149‑1 (MQ=255)
                                                                      cAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTg                                                                             >  8:88911/1‑143 (MQ=255)
                                                                      cAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTg                                                                             <  7:88911/143‑1 (MQ=255)
                                                                      cAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAacac                                                                       >  6:125851/1‑149 (MQ=255)
                                                                       aGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACAcc                                                                      >  5:141993/1‑149 (MQ=255)
                                                                       aGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACAcc                                                                      <  2:35093/149‑1 (MQ=255)
                                                                              gATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCa                                                               >  5:136368/1‑149 (MQ=255)
                                                                                              aaCAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTg                                                                                     <  3:113641/111‑1 (MQ=255)
                                                                                              aaCAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTg                                                                                     >  4:113641/1‑111 (MQ=255)
                                                                                              aaCAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAgc                                               >  4:172444/1‑149 (MQ=255)
                                                                                               aCAGCGACGGCACGCTCACCTTCTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAgcg                                              >  6:68701/1‑149 (MQ=255)
                                                                                               aCAGCGACGGCACGCTCACCTACTCGTTAGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAgcg                                              >  8:170038/1‑149 (MQ=255)
                                                                                                 aGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCt                                            <  6:136368/149‑1 (MQ=255)
                                                                                                  gCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCtt                                           <  2:193863/149‑1 (MQ=255)
                                                                                                      cGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACg                                       >  7:306351/1‑149 (MQ=255)
                                                                                                             cTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GCCCGCGCGATCTTCCTCAGCCCAATATACCTGAAGGCCAAGAACACCTGGCCCAACGTCCGTACGACAGCTCATGACgaagacc                                 >  4:191241/1‑142 (MQ=255)
                                                                                                                acgtACGCGGTCGTGGATACCGTGGTCGCCATCCACCCAGGCGTCGTCGTTGGCGTCGTC‑GGCGGCGCGATCTTGCTCAGCGGCTTGTTCCTGATGGCCTGGAACACCTGGCTCACAGTGCGTTCGCCAGCGGTTGAGGTGGCCCCTGc                             <  4:272702/146‑1 (MQ=255)
                                                                                                                aCCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGTCCTGGAACACCTGGAGCACCGTGCGTTCGCCAGCGGATGACGTCGCACCTGc                             >  8:266620/1‑149 (MQ=255)
                                                                                                                aCCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTg                              <  1:237878/148‑1 (MQ=255)
                                                                                                                aCCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGAGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGc                             <  7:170038/149‑1 (MQ=255)
                                                                                                                  cTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCg                            >  3:278709/1‑148 (MQ=255)
                                                                                                                   tACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGaa                          >  3:182265/1‑149 (MQ=255)
                                                                                                                     cTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACg                        >  5:71136/1‑149 (MQ=255)
                                                                                                                      tcgttcgtGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGc                       >  8:149946/1‑149 (MQ=255)
                                                                                                                         cacgacgAAGCCTGGGTGGCTGGCCACCAAGGTTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGcc                      >  8:42793/7‑147 (MQ=38)
                                                                                                                           cgtGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAg                   <  6:141993/148‑1 (MQ=255)
                                                                                                                           cgacgAAGCCTGGGTGGCTGGCCACCAAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGt                  >  4:191732/5‑149 (MQ=255)
                                                                                                                            gtGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGtt                 >  7:135460/1‑149 (MQ=255)
                                                                                                                            gtGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGtt                 >  6:129004/1‑149 (MQ=255)
                                                                                                                             tGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTg                <  3:172444/149‑1 (MQ=255)
                                                                                                                              ggAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTgg               >  4:58593/1‑149 (MQ=255)
                                                                                                                               gAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGc              <  3:58593/149‑1 (MQ=255)
                                                                                                                                 aaCCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCTCCCCTGCGAACGCCCAGTTGGCtt            <  5:65172/149‑1 (MQ=255)
                                                                                                                                  aCCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCtt            >  6:163410/1‑148 (MQ=255)
                                                                                                                                   ccTGGGTGGCTGGCCACCAAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCTTga          <  3:191732/149‑1 (MQ=255)
                                                                                                                                    cTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCGCCTGCGAACGCCCAGTTGGCTTgag         <  1:304496/149‑1 (MQ=255)
                                                                                                                                    cTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCTTgag         >  2:60407/1‑149 (MQ=255)
                                                                                                                                    cTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCCGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCTTgag         <  8:165841/149‑1 (MQ=255)
                                                                                                                                        gtggCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCTTgaggag      <  5:125851/148‑1 (MQ=255)
                                                                                                                                           gccagccaCCCAGGCTTCGTCGTGCGCTTCGTCGGGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCTTGAGGAGaaa   >  1:48954/1‑149 (MQ=255)
                                                                                                                                           gccagccaCCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGTGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCTTGAGGAGAAAc  <  8:29245/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TGCGCACTTCTGGCTGGCCACCATCGGTACCGTGCTGTACATCGCCTCGATGTGGGTCAACGGCATCGCCCAGGGCCTGATGTGGCGCGCGGTCAACAGCGACGGCACGCTCACCTACTCGTTCGTGGAAACCTTGGTGGCCAGCCACCCAGGCTTCGTCGTGCGCTTCGTC‑GGCGGCGCGATCTTCCTCAGCGGCATGTTCCTGATGGCCTGGAACACCTGGCGCACCGTGCGTTCGCCAGCGCTTGACGTCGCCCCTGCGAACGCCCAGTTGGCTTGAGGAGAAAC  >  NC_002947/4837139‑4837426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑