Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	367,367	T→G	9.5%	intergenic (‑53/+21)	PP_5442 ← / ← PP_0306	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	367,367	0	T	G	9.5%	148.3 / 3.7	58	intergenic (‑53/+21)	PP_5442/PP_0306	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/22);  new base G (3/3);  total (33/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCAAATCTTCCCATGTATCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAGCGCGATAGACGCGGGTAGAGGTTTTGT  >  NC_002947/367219‑367515                                                                                                                                                      |                                                                                                                                                     cTGCAAATCTTCCCATGTATCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGct                                                                                                                                                      >  5:82931/1‑149 (MQ=255) cTGCAAATCTTCCCATGTATCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGct                                                                                                                                                      <  2:27362/149‑1 (MQ=255)  tGCAAATCTTCCCATGTATCGAGTGTCAGCCAGGGCGCAGGCTTCATGCTTACCCGCTCCTCGAATATTCGGGTGTCCACGCTCG‑TATCGTCCATGTGCCATTGCTCCTTGATGGATGCAGATGCCCAGAACCCGGTACAAGCGctcta                                                                                                                                                     >  7:132280/1‑148 (MQ=255)    cAAATCTTCCCATGTATCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTg                                                                                                                                                   <  4:156692/149‑1 (MQ=255)            cccATGTATCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATCTTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTAc                                                                                                                                               <  4:9246/145‑1 (MQ=255)            cccATGTATCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCgg                                                                                                                                           >  5:302335/1‑149 (MQ=255)            cccATGTATCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGAGCGCGCTGGTAc                                                                                                                                               <  1:295570/145‑1 (MQ=255)              cATGTATCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGc                                                                                                                                          >  3:228540/1‑148 (MQ=255)               aTGTATCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCt                                                                                                                                        >  5:2505/1‑149 (MQ=255)                    tCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTAccc                                                                                                                                             >  8:211258/1‑139 (MQ=255)                    tCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTAccc                                                                                                                                             <  7:211258/139‑1 (MQ=255)                    tCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCt                                                                                                                                    >  6:75856/1‑148 (MQ=255)                      gAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTaa                                                                                                                                  >  8:257069/1‑148 (MQ=255)                        gtgtCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCa                                                                                                                               <  6:2505/149‑1 (MQ=255)                         tgtCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGTGTGTCCAGGCTAG‑TATCGTCCATTTGGCATTGCTCCTTGATTGATTCAGACTACCAGAACCGGCTAACAGCGAGCTCTTGTACACTGCCTCTCTAAt                                                                                                                                 >  4:90248/1‑146 (MQ=255)                                cAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCga                                                                                                                       <  6:82931/149‑1 (MQ=255)                                  gtgcgcAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGGCCATGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCgaga                                                                                                                     <  8:248310/147‑1 (MQ=255)                                          gCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGAAAGCGACAAACGcccc                                                                                                             >  2:185906/1‑149 (MQ=255)                                              gATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCATATAGCGAGAAAACCGCCGAgg                                                                                                         >  8:141013/1‑149 (MQ=255)                                                       ccGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTg                                                                                                <  7:141013/149‑1 (MQ=255)                                                         gCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGa                                                                                               >  4:27545/1‑148 (MQ=255)                                                           tcctcGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGAtct                                                                                            >  5:98406/1‑149 (MQ=255)                                                             ctcGAATATTCGGGTGTCCAGGCTCGTTATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGAtctt                                                                                           >  1:14995/1‑149 (MQ=255)                                                             ctcGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGAtcttc                                                                                          <  6:302335/149‑1 (MQ=255)                                                             ctcGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGAtcttc                                                                                          <  3:27545/149‑1 (MQ=255)                                                                gAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCa                                                                                       >  8:52775/1‑149 (MQ=255)                                                                     ttCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTca                                                                                   <  3:143062/148‑1 (MQ=255)                                                                        gggTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACaa                                                                                <  7:257069/148‑1 (MQ=255)                                                                          gtgtCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGt                                                                             >  6:301548/1‑149 (MQ=255)                                                                                aGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTg                                                                       <  2:14995/149‑1 (MQ=255)                                                                                  gCTCT‑TATCGTCCATGTGGCATTGCTCATTGATGGATTCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGATACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCa                                                                     <  8:132280/149‑1 (MQ=255)                                                                                          tCGTCCATGTGGCATTGCTCCTTGATGGATGCAGACGACCAGAACCGGGTACCAGAGCGCGCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCg                                                               <  3:90248/148‑1 (MQ=255)                                                                                             tCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGCTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTAGAAGt                                                           >  4:175048/1‑149 (MQ=255)                                                                                               cATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGa                                                         >  8:141554/1‑149 (MQ=255)                                                                                                   tgGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGaa                                                     >  5:221263/1‑149 (MQ=255)                                                                                                   tgGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGaa                                                     >  4:203240/1‑149 (MQ=255)                                                                                                     gCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACg                                                   <  1:185906/149‑1 (MQ=255)                                                                                                       aTTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCt                                                 >  3:309859/1‑149 (MQ=255)                                                                                                            tCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACg                                            >  7:31719/1‑149 (MQ=255)                                                                                                             ccTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGt                                           >  7:97374/1‑149 (MQ=255)                                                                                                              cTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGt                                           <  5:75856/148‑1 (MQ=255)                                                                                                              cTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTg                                          >  1:287322/1‑149 (MQ=255)                                                                                                              cTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTg                                          >  2:291041/1‑149 (MQ=255)                                                                                                                tGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGcc                                        >  4:40528/1‑149 (MQ=255)                                                                                                                     gatgCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCttgtt                                   <  8:31719/149‑1 (MQ=255)                                                                                                                        gCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCg                                >  2:124697/1‑149 (MQ=255)                                                                                                                          aGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCg                                >  5:28134/1‑147 (MQ=255)                                                                                                                             tGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAgcg                           <  1:291041/149‑1 (MQ=255)                                                                                                                             tGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAgcg                           <  7:52775/149‑1 (MQ=255)                                                                                                                              gACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAgcgc                          >  2:90049/1‑149 (MQ=255)                                                                                                                                 cAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGGAGCGCGAt                       <  1:124697/149‑1 (MQ=255)                                                                                                                                   aggccGGGTACCAGAGCGCGCTGGTACCCGGTCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAGCGCGa                        >  6:106853/4‑146 (MQ=255)                                                                                                                                      ccGGGTACCAGAGCGCGCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAGCGCGATAGAcg                  >  5:148512/1‑149 (MQ=255)                                                                                                                                       cGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAGCGCGATAGAc                   >  8:250432/1‑147 (MQ=255)                                                                                                                                       cGGGTACCAGAGCGCGCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAGCGCGATAGAcgc                 >  7:205749/1‑149 (MQ=255)                                                                                                                                         ggTACCAGCGCGCGCTGCTACCCGGCCTCCCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAGCGCGATAGACGCgg               <  8:246774/149‑1 (MQ=255)                                                                                                                                              cAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGTGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAGCGCGATAGACGCGGGTa            >  6:279738/1‑147 (MQ=255)                                                                                                                                              cAGCGCGCTCTGGTACCCGGCCTCGCTAAACAGATAGCGAGAAACGCGCCGAGGTCCCCATAGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGATGCGAAGCGCGATAGACGCGGGTa            >  1:190476/1‑147 (MQ=255)                                                                                                                                               aGCCCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGGCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAGCGCGATAGACGCGGGTagag         <  4:46049/149‑1 (MQ=255)                                                                                                                                                  gcgcTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAGCGCGATAGACGCGGGTAGAGGtt      <  6:221263/149‑1 (MQ=255)                                                                                                                                                    gcTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAGCGCGATAGACGCGGGTAGAGGtttt    <  3:40528/149‑1 (MQ=255)                                                                                                                                                      tctGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAGCGCGATAGACGCGGGTAGAGGTTTTGt  >  3:258551/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                     CTGCAAATCTTCCCATGTATCGAGTGTCAGCCAGGGCGCAGGCTTGATGCTTAGCCGCTCCTCGAATATTCGGGTGTCCAGGCTCG‑TATCGTCCATGTGGCATTGCTCCTTGATGGATGCAGATGACCAGAACCGGGTACCAGCGCGCTCTGGTACCCGGCCTCGCTAATCAGATAGCGAGAAACGCGCCGAGGTCGCCTTTGATCTTCTCATTCACAAGGTCACGTGCAGCGTCGAAGTGACGAACGCTGAACGTGCCTTGTTGCGAAGCGCGATAGACGCGGGTAGAGGTTTTGT  >  NC_002947/367219‑367515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑