| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,936,630 | G→C | 7.1% | intergenic (‑41/‑377) | flhA ← / → PP_4345 | flagellar export pore protein/GntR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,936,630 | 0 | G | C | 7.1% | 166.5 / 3.6 | 56 | intergenic (‑41/‑377) | flhA/PP_4345 | flagellar export pore protein/GntR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (27/25); new base C (2/2); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
TCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGAGCAAGCAATCACCAAGAAAAACGAGACCTCCCGCCCAATCG > NC_002947/4936484‑4936769 | tcatcatTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGcc < 8:201624/149‑1 (MQ=34) catcatTGAGAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCACGTTGTTGCGGGCGTTGCAGATTAACTGAGAGCGATCCAACGCGAGTCCTCTTCATGCAAAACTTTGACGCCCAGCGGGCGCCt > 4:220412/1‑149 (MQ=11) atcatTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGAAGCAAAACTTTGACGCCCAGGGGGCGCCt < 1:183030/148‑1 (MQ=21) atTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGc < 1:154791/149‑1 (MQ=37) atTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGc < 8:35209/149‑1 (MQ=37) aaCATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGCGCCCCCTGGGCGtcaaa < 1:300763/149‑5 (MQ=255) cATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCttt < 4:153533/149‑1 (MQ=255) cATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCttt < 2:209001/149‑1 (MQ=255) aTCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTg < 1:179563/149‑1 (MQ=255) aCCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGc > 8:117830/1‑147 (MQ=255) ccAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGc > 8:216065/1‑146 (MQ=255) ccAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGc > 5:316549/1‑146 (MQ=255) agcagcAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCaagaa < 1:9230/149‑1 (MQ=255) gcaACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑tt < 5:110511/149‑1 (MQ=255) caACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTgg < 7:173044/133‑1 (MQ=39) caACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTgg > 8:173044/1‑133 (MQ=39) aaCAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTc < 5:211220/149‑1 (MQ=255) aGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCaa > 6:69762/1‑149 (MQ=255) gCACCCACAGGTTGCCCAGGCCTAGCCCGGCCAGGGTGTTGCGTGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACttt < 4:98598/149‑1 (MQ=14) aCCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTgg < 3:145320/149‑1 (MQ=255) ccccAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTgg > 2:272235/1‑147 (MQ=255) cAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGa > 3:102419/1‑148 (MQ=255) cAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGCCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGac < 4:29121/149‑1 (MQ=255) cctggCCGGGCTCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCacac > 3:122470/4‑149 (MQ=255) cGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGCCACCCACACGaa > 8:233716/1‑146 (MQ=255) cGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGaa > 6:128173/1‑146 (MQ=255) aGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGa < 6:126819/148‑1 (MQ=255) aGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGa > 5:126819/1‑148 (MQ=255) cGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAAc > 7:6117/1‑149 (MQ=255) gCCGGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCg > 3:256589/1‑149 (MQ=255) gttgCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGc < 7:216065/148‑1 (MQ=255) gCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGcacca > 5:259060/1‑149 (MQ=255) tGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACg > 2:222111/1‑149 (MQ=255) ttAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGt < 5:69762/149‑1 (MQ=255) aGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCAt > 5:220636/1‑149 (MQ=255) tGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGa < 1:222111/149‑1 (MQ=255) tCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGCCGAGCACCACCGCGTTGACGGGTGGCTCATGagcaag > 3:202778/1‑149 (MQ=255) ccACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGagcaag > 4:171555/1‑148 (MQ=255) cACCGCTAGTGCTGTTGATCCAAAACTTTGAAGCCCAGGGGGCGCCTGGGCGTTGCTTTGCAAGAAGG‑TTTCCAACTTTGGCAGACACCCACAGGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGagcaagc < 3:220412/148‑1 (MQ=255) cACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGagcaagc < 4:122470/148‑1 (MQ=255) aCCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCAt < 8:142745/139‑1 (MQ=255) aCCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCAt > 7:142745/1‑139 (MQ=255) ccGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGAGCAAGCAAt > 2:7758/1‑149 (MQ=255) cgcgAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGAGCAAGCAATc < 4:102419/149‑1 (MQ=255) cAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGAGCAAGCAATCACCAAGAAAAACgaga < 1:7758/148‑1 (MQ=255) aaaaCTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCTGGCACCCACACGAAAAGATGAACCGGGCGAGCACCACCGGGTTGACGGGTGGCTCATGAGCAAGCAATCAGCAAGAAAAACGAGccc > 2:262183/1‑149 (MQ=38) aaaaCTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGAGTGGCTCATGAGCAAGCAATCACCAAGAAAAACGAGAcc > 4:206581/1‑149 (MQ=255) aaaaCTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCCAGCACCACCGCGTTGACGGGTGGCTCAAGAGCAAGCAATCACAAAGAAACACGAGAcc > 2:88205/1‑149 (MQ=255) tGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGCTGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGAGCAAGCAATCACCAAGAAAAACGAGACCTCCCGc < 4:256589/148‑1 (MQ=255) ccaCGCCCAGGCGCCCCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGAGCAAGCAATc < 7:262858/122‑1 (MQ=255) ccaCGCCCAGGCGCCCCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGAGCAAGCAATc > 8:262858/3‑124 (MQ=255) aCGCCCAGGCGCCCCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGAGCAAGCAATCACCAAGAAAAACGAGACCTCCCGccc > 4:43977/1‑148 (MQ=255) gCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGAGCAAGCAATCACCAAGAAAAACGAGACCTCCAGCACAAt > 2:109039/1‑149 (MQ=255) cccAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGAGCAAGCAATCACCAAGAAAAACGAGACCTCCCGCCCAATc > 3:150417/1‑149 (MQ=255) cacAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGCTTTTCCTACTTTGGCAGACACCAACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTTGCTCATGAGCAAGCAATCACCAAGAAAAACGAGACCTCCCTCCCAAt < 1:109039/147‑1 (MQ=255) ccAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAGGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGAGCAAGCAATCACCAAGAAAAACGAGACCTCCCGCCCAATCg > 7:55921/1‑149 (MQ=255) | TCATCATTGCCAACATCACCAGCAGCAACAGCGGCACCCCCAGGTTGCCCCGGCCGAGCCCGGCCAGGTTGTTGCGGGCGTTGCTGATTAACTGAGTGCGATCCACCGCGAGTCCTCTTGATGCAAAACTTTGACGCCCAGGGGGCGCCTGGGCGTGGCTTTGCAAGAAGC‑TTTCCAACTTTGGCAGACACCCACACGAAAAGATGAACCGGGCGAGCACCACCGCGTTGACGGGTGGCTCATGAGCAAGCAATCACCAAGAAAAACGAGACCTCCCGCCCAATCG > NC_002947/4936484‑4936769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |