Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,937,497	A→G	6.1%	D164G (GAC→GGC)	PP_4345 →	GntR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,937,497	0	A	G	6.1%	190.4 / 2.2	65	D164G (GAC→GGC)	PP_4345	GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (34/27);  new base G (2/2);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

GCGCAGATGTACGAACTCTTATCTACCATGGAGCATGCCACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGCCGAGCACCTTGCCGAG  >  NC_002947/4937355‑4937642                                                                                                                                                |                                                                                                                                                  gcgcAGATGTACGAACTCTTATCTACCATGGAGCATGCCACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTc                                                                                                                                                <  1:322429/146‑1 (MQ=255) gcgcAGATGTACGAACTCTTATCTACCATGGAGCATGCCACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGt                                                                                                                                             <  1:283047/149‑1 (MQ=255)           aCGAACTCTTATCTACCATGGAGCATGCCACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCAt                                                                                                                                   >  8:83155/1‑149 (MQ=255)               aCTCTTATCTACCATGGAGCATGCCACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTc                                                                                                                               >  8:191389/1‑149 (MQ=255)               aCTCTTATCTACCATGGAGCATGCCACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTc                                                                                                                               <  7:191389/149‑1 (MQ=255)                 tctTATCTACCATGGAGCATGCCACCGACCCGCACGACCTTAGCGAACTCAATGAAAAGTTTCATGACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGt                                                                                                                             >  1:177293/1‑149 (MQ=255)                 tctTATCTACCATGGAGCATGCCACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTATGGGGAATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCg                                                                                                                              >  1:272443/1‑149 (MQ=255)                   ttATCTACCATGGAGCATGCCACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGttt                                                                                                                           >  1:153138/1‑149 (MQ=255)                          ccATGGAGCATGCCACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGtttttt                                                                                                                        <  1:254329/145‑1 (MQ=255)                          ccATGGAGCATGCCACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTttgt                                                                                                                      <  8:52005/147‑1 (MQ=255)                           cATGGAGCATGCCACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGa                                                                                                                   >  5:180112/1‑149 (MQ=255)                                      ccACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGa                                                                                                         <  7:83155/148‑1 (MQ=255)                                      ccACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGa                                                                                                         <  3:163346/148‑1 (MQ=255)                                       cACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTttgt                                                                                                                      <  7:190083/134‑1 (MQ=255)                                       cACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTttgt                                                                                                                      >  8:190083/1‑134 (MQ=255)                                       cACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGAc                                                                                                        >  2:73383/1‑148 (MQ=255)                                              ccGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATACAAAATCAAAGCGCCCCAGATTA‑CTTGCATTGATTAATTCCCTTCATGACTCGGTGAATCGCCATCTTCGTTATTTGTTCACAAAACTCGAcagaaagc                                                                                                 >  2:113115/1‑141 (MQ=255)                                                        ttAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCaa                                                                                      >  6:319492/1‑149 (MQ=255)                                                            cGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAgg                                                                                  <  2:177293/149‑1 (MQ=255)                                                                 tCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCa                                                                              >  4:161615/1‑148 (MQ=255)                                                                   aaTGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGGCTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAAc                                                                                     <  6:300915/139‑1 (MQ=255)                                                                   aaTGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGGCTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAAc                                                                                     <  6:296056/139‑1 (MQ=255)                                                                   aaTGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGGCTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAAc                                                                                     >  5:300915/1‑139 (MQ=255)                                                                   aaTGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGGCTCGGTGGATCGACATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAAc                                                                                     >  5:296056/1‑139 (MQ=255)                                                                   aaTGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATa                                                                            >  5:199228/1‑148 (MQ=255)                                                                     tGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGa                                                                                                                   >  6:291248/1‑107 (MQ=255)                                                                     tGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGa                                                                                                                   <  5:291248/107‑1 (MQ=255)                                                                       aaaaGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAg                                                                        >  3:198541/1‑148 (MQ=255)                                                                              tcatcaCTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTg                                                                 <  1:129023/148‑1 (MQ=255)                                                                               catcaCTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAg                                                               >  8:296647/1‑149 (MQ=255)                                                                                atcaCTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTAGACTATCACAAGAAATCGCAACAGGATCAAAAAAGCATTTTGAgc                                                              >  8:131038/1‑149 (MQ=255)                                                                                atcaCTTTCTTTATTCAAAACCAAACCGCCCCAGATTA‑CTAGCAATGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAgc                                                              <  8:277820/149‑1 (MQ=255)                                                                                  caCTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAgcgc                                                            >  7:141612/1‑149 (MQ=255)                                                                                     tttctttATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTg                                                         >  7:134042/1‑149 (MQ=255)                                                                                     tttctttATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTg                                                         >  4:111248/1‑149 (MQ=255)                                                                                             ttCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCTCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAgg                                                 >  4:202533/1‑149 (MQ=255)                                                                                             ttCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAgg                                                 <  2:272443/149‑1 (MQ=255)                                                                                             ttCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTACCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAgg                                                 >  3:127393/1‑149 (MQ=255)                                                                                               cAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGt                                               >  8:141337/1‑149 (MQ=255)                                                                                                aaaaGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAgg                                                 <  1:50275/146‑1 (MQ=255)                                                                                                aaaaGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAgg                                                 >  2:50275/1‑146 (MQ=255)                                                                                                  aagcaaagcGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGAt                                            >  5:169588/1‑149 (MQ=255)                                                                                                    gcaaagcGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATcg                                          >  2:113750/1‑149 (MQ=255)                                                                                                     caaagcGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATcgc                                         <  6:199228/149‑1 (MQ=255)                                                                                                         gcgcCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAg                                     <  8:141612/149‑1 (MQ=255)                                                                                                              ccAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAgccgcc                                <  2:153138/149‑1 (MQ=255)                                                                                                                aGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTTACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCACCaa                              <  4:305848/149‑1 (MQ=255)                                                                                                                          gCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAAtttt                      >  7:69419/1‑148 (MQ=255)                                                                                                                           cATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTa                    <  6:180112/149‑1 (MQ=255)                                                                                                                           cATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCATCGAAATTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGAATCATAAAAGCATTTTGAGCGCTTTCGCCAAGGGTGATCGCGAAACCTCCAAGCAAATTTTa                    >  2:283384/1‑149 (MQ=255)                                                                                                                            aTTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAg                   <  5:319492/149‑1 (MQ=255)                                                                                                                               gATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGcc                 <  4:198541/148‑1 (MQ=255)                                                                                                                                 ttAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAAttttt                     <  2:102143/142‑1 (MQ=255)                                                                                                                                 ttAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAAAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAAttttt                     >  1:102143/1‑142 (MQ=255)                                                                                                                                    aGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCTAAGCCGCCAAGCAATTTTTAGCCGAGCAc           >  8:1298/1‑149 (MQ=255)                                                                                                                                    aGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGCCGAGCAc           <  7:296647/149‑1 (MQ=255)                                                                                                                                    aGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGCCGAGCAc           >  3:127954/1‑149 (MQ=255)                                                                                                                                    aGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGCCGAGCAc           >  4:310725/1‑149 (MQ=255)                                                                                                                                    aGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGCCGAGCAc           >  8:5998/1‑149 (MQ=255)                                                                                                                                     gTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGCCGAGCAcc          >  5:272893/1‑149 (MQ=255)                                                                                                                                        ccTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGCCGAGCACCTTg       <  6:272893/149‑1 (MQ=255)                                                                                                                                         cTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGCCGAGCAcc          <  7:251834/145‑1 (MQ=255)                                                                                                                                         cTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGCCGAGCAcc          >  8:251834/1‑145 (MQ=255)                                                                                                                                            cATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGCCGAGCACCTTGCCg    <  1:113750/148‑1 (MQ=255)                                                                                                                                             aTGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGCCGAGCACCTTGCCGAg  <  4:127954/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                  GCGCAGATGTACGAACTCTTATCTACCATGGAGCATGCCACCGACCCGCACCACCTTAGCGAACTCAATGAAAAGTTTCATCACTTTCTTTATTCAAAAGCAAAGCGCCCCAGATTA‑CTTGCATTGATTAAGTCCCTTCATGACTCGGTGGATCGCCATCTTCGTTTTTTGTTGACAAATCTCGACTATCACAAGAAATCGCAACAGGATCATAAAAGCATTTTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGCCGAGCACCTTGCCGAG  >  NC_002947/4937355‑4937642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑