Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,948,990	C→A	6.6%	G119V (GGC→GTC)	fliN ←	flagellar basal‑body C‑ring protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,948,990	0	C	A	6.6%	231.1 / 4.0	73	G119V (GGC→GTC)	fliN	flagellar basal‑body C‑ring protein
Reads supporting (aligned to +/- strand):  ref base C (30/38);  new base A (2/3);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

GCGCATCGTGCCCTTCACTCAGCGCAGCTTCTTGATACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCTGACATTTTC  >  NC_002947/4948842‑4949138                                                                                                                                                        |                                                                                                                                                      gcgcATCGTGCCCTTCACTCAGCGCAGCTTCTTGATACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAAGTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGc                                                                                                                                                       >  2:261046/1‑149 (MQ=255) gcgcATCGTGCCCTTCACTCAGCGCAGCTTCTTGATACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGc                                                                                                                                                       >  4:288181/1‑149 (MQ=255)        gTGCCCTTCACTCAGCGCAGCTTCTTGATACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAg                                                                                                                                                <  7:155734/149‑1 (MQ=255)             cTTCACTCAGCGCAGCTTCTTGATACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGt                                                                                                                                           >  1:213200/1‑149 (MQ=255)              ttCACTCAGCGCAGCTTCTTGATACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTc                                                                                                                                          >  1:315389/1‑149 (MQ=255)                 aCTCAGCGCAGCTTCTTGATACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGa                                                                                                                                        <  8:31486/148‑1 (MQ=255)                        gcAGCTTCTTGATACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGAt                                                                                                                                 >  7:130781/1‑148 (MQ=255)                            cttcttGATACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGCGCTCGATCAcc                                                                                                                             >  8:245217/1‑148 (MQ=255)                             ttcttGATACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCAcc                                                                                                                             >  1:70283/1‑147 (MQ=255)                               cttGATACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGa                                                                                                                           <  5:37724/147‑1 (MQ=255)                                  gATACGTTCGCTGGGGCTGATCACGTCAGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGcc                                                                                                                        <  7:37322/147‑1 (MQ=255)                                    tACTTTCGCTGGGGCTGATGACGTAGTTCAGGCGTATGCCGTACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGAGTGCCGTTGACCAGCAC‑‑‑GTGGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGGTGATCGAGctcga                                                                                                                     <  5:243754/148‑5 (MQ=255)                                    tACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑g                                                                                                                    <  7:178971/148‑1 (MQ=255)                                       gTTCGCTGGGGCTGATCCCGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGtt                                                                                                                 >  3:138109/1‑148 (MQ=255)                                       gTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGtt                                                                                                                 >  7:21472/1‑148 (MQ=255)                                       gTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTg                                                                                                                <  3:256532/149‑1 (MQ=255)                                            cTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTg                                                                                                           <  4:31186/149‑1 (MQ=255)                                               gggCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCAcc                                                                                                                             >  6:175357/1‑129 (MQ=255)                                               gggCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCAcc                                                                                                                             <  5:175357/129‑1 (MQ=255)                                               gggCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTgca                                                                                                         <  2:315389/148‑1 (MQ=255)                                                 gCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTgcag                                                                                                        >  5:106012/1‑147 (MQ=255)                                                 gCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTgcag                                                                                                        >  4:142822/1‑147 (MQ=255)                                                  cTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGGTCGACCGCCTGGCCGGCGAGc                                                                                                                                      >  5:328244/1‑117 (MQ=255)                                                  cTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGACCGCCTGGCCGGCGAGc                                                                                                                                      <  6:328244/117‑1 (MQ=255)                                                     aTCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAgg                                                                                                    <  2:213200/147‑1 (MQ=255)                                                     aTCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGtt                                                                                                  >  3:286403/1‑149 (MQ=255)                                                     aTCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGtt                                                                                                  >  2:134132/1‑149 (MQ=255)                                                      tCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGtt                                                                                                                 <  3:16314/133‑1 (MQ=255)                                                      tCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGtt                                                                                                                 >  4:16314/1‑133 (MQ=255)                                                          gTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGGTGAGCTGCAGCAGGTTGCGGa                                                                                             <  8:100714/149‑1 (MQ=255)                                                             ggTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGt                                                                                          <  7:69763/149‑1 (MQ=255)                                                             ggTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGt                                                                                          <  8:130781/149‑1 (MQ=255)                                                               tCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCgg                                                                                                                                                    <  2:145158/90‑1 (MQ=255)                                                               tCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTg                                                                                        <  1:134132/149‑1 (MQ=255)                                                                  ggCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGAtc                                                                                     <  3:142822/149‑1 (MQ=255)                                                                          ccGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCAGCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGtgct                                                                             >  3:302494/1‑149 (MQ=255)                                                                           cGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGc                                                                                                                                                   >  6:1769/1‑79 (MQ=255)                                                                           cGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGc                                                                                                                                                   <  5:1769/79‑1 (MQ=255)                                                                           cGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTgca                                                                                                         >  3:97621/1‑120 (MQ=255)                                                                           cGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTgca                                                                                                         <  4:97621/120‑1 (MQ=255)                                                                           cGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGtgctg                                                                            <  3:288181/149‑1 (MQ=255)                                                                                ttttCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCAc                                                                       >  8:152348/1‑149 (MQ=255)                                                                                 tttCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACt                                                                      >  4:4166/1‑149 (MQ=255)                                                                                   tagTTGACCACGAGCACTTCGCCTTGGGCGATCAGCGTGCAGTTGACCAGCCCTCTGTCGTCCCGCTCGACGGCCAGGCGGTGGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCTGTGCTGCCCa                                                                        <  1:105741/146‑1 (MQ=255)                                                                                     gTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCa                                                                  >  2:123209/1‑149 (MQ=255)                                                                                          ccacgaccacTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGtt                                                                                         <  3:54415/121‑1 (MQ=255)                                                                                          ccacgaccacTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGtt                                                                                         >  4:54415/1‑121 (MQ=255)                                                                                          ccacgaccacTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGa                                                              >  6:91715/1‑148 (MQ=255)                                                                                           cacgaccacTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGaaa                                                            >  6:77162/1‑149 (MQ=255)                                                                                               accacTTCGCCATCGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGtt                                                        <  2:45625/149‑1 (MQ=255)                                                                                                ccacTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGtt                                                        >  4:218468/1‑148 (MQ=255)                                                                                                   cTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCTCTGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCAc                                                     <  1:261046/149‑1 (MQ=255)                                                                                                   cTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCAcc                                                    <  7:152348/149‑1 (MQ=255)                                                                                                   cTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCAcc                                                    >  1:302480/1‑149 (MQ=255)                                                                                                    ttCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCg                                                   >  3:75266/1‑149 (MQ=255)                                                                                                       gCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGt                                                                                >  4:277312/1‑117 (MQ=255)                                                                                                       gCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGt                                                                                <  3:277312/117‑1 (MQ=255)                                                                                                       gCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGTTGCCCACTTCCATGGAAATGTTCACCGGGa                                                <  4:138109/149‑1 (MQ=255)                                                                                                             ggCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTcc                                           <  2:302480/148‑1 (MQ=255)                                                                                                               cGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAgg                                        <  5:91715/149‑1 (MQ=255)                                                                                                               cGATCAGCGGGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGATGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAgg                                        <  2:234170/149‑1 (MQ=255)                                                                                                                  tCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGAt                                      >  4:176053/1‑148 (MQ=255)                                                                                                                  tCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATc                                     <  4:155128/149‑1 (MQ=255)                                                                                                                          ccGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAg                             <  4:286403/149‑1 (MQ=255)                                                                                                                          ccGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAg                             <  8:21472/149‑1 (MQ=255)                                                                                                                            gTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGt                           >  5:113328/1‑149 (MQ=255)                                                                                                                            gTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGt                           <  2:70283/149‑1 (MQ=255)                                                                                                                                       c‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCAGCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCGATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCg                <  4:302494/149‑1 (MQ=255)                                                                                                                                            tCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAg              <  2:214872/149‑1 (MQ=255)                                                                                                                                                 gagCTCGACCGCCTGGCCGGCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCt           >  3:214862/3‑147 (MQ=255)                                                                                                                                                 gagCTCGACCGCCTGGCCGGCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCt           <  4:214862/145‑1 (MQ=255)                                                                                                                                                   gCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCggg                      <  4:44785/134‑1 (MQ=255)                                                                                                                                                   gCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCggg                      >  3:44785/1‑134 (MQ=255)                                                                                                                                                    cTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCTGACAt      <  6:106012/149‑1 (MQ=255)                                                                                                                                                     tCGCCGGCCAGGCGGTAGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATCTAGATCTCGGTGCTGCCCAATTCCATGTAAATGAACACCCGGATGACCAGGATCACCTCCAGTTTCGGGCCTTCGAGGATGACAtt     >  6:236203/1‑149 (MQ=255)                                                                                                                                                        ccGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCTGACATTTTc  <  1:123209/149‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                      GCGCATCGTGCCCTTCACTCAGCGCAGCTTCTTGATACGTTCGCTGGGGCTGATCACGTCGGTCAGGCGGATGCCGAACTTTTCGTTGACCACGACCACTTCGCCATGGGCGATCAGCGTGCCGTTGACCAGCAC‑‑‑GTCGAGCGGCTCGCCGGCCAGGCGGTCGAGCTCGATCACCGAGCCCT‑GGTTGAGCTGCAGCAGGTTGCGGATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCTGACATTTTC  >  NC_002947/4948842‑4949138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑