Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,183,245 G→C 5.6% F163L (TTC→TTG)  PP_4563 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,183,2450GC 5.6% 233.8 / 3.2 72F163L (TTC→TTG) PP_4563hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (36/32);  new base C (2/2);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

GCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGCACCACG  >  NC_002947/5183099‑5183389
                                                                                                                                                  |                                                                                                                                                
gcgcCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGaa                                                                                                                                                <  5:249105/149‑1 (MQ=255)
 tgcCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAg                                                                                                                                               <  1:98921/148‑1 (MQ=255)
     tACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCa                                                                                                                                           >  4:256455/1‑149 (MQ=255)
       ccGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGccc                                                                                                                                            <  5:305563/146‑1 (MQ=255)
       ccGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGccc                                                                                                                                            >  6:305563/1‑146 (MQ=255)
           cTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGGCTTCAAGGCCATcatca                                                                                                                                     <  3:33395/149‑5 (MQ=255)
           cTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGGCTTCAAGGCCATcatca                                                                                                                                     <  3:29347/149‑5 (MQ=255)
               aGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTc                                                                                                                                 <  6:230387/149‑1 (MQ=255)
                 gCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGc                                                                                                                               >  8:150045/1‑149 (MQ=255)
                   tCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCg                                                                                                                             >  7:40133/1‑149 (MQ=255)
                     aGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCg                                                                                                                             <  8:10502/147‑1 (MQ=255)
                     aGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCg                                                                                                                             <  3:168871/147‑1 (MQ=255)
                      gTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGttt                                                                                                                                  <  1:323468/141‑1 (MQ=255)
                      gTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGttt                                                                                                                                  >  2:323468/1‑141 (MQ=255)
                       tGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGcca                                                                                                                         >  2:123081/1‑149 (MQ=255)
                            tCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACcag                                                                                                                     >  7:265340/1‑148 (MQ=255)
                              ttGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACcagcag                                                                                                                  >  7:224783/1‑149 (MQ=255)
                                gCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCt                                                                                                                >  3:53753/1‑149 (MQ=255)
                                    tCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGAc                                                                                                            <  3:256455/149‑1 (MQ=255)
                                    tCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGAc                                                                                                            <  1:123081/149‑1 (MQ=255)
                                         gcgcACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCt                                                                                                       >  6:229934/1‑149 (MQ=255)
                                             aCTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTc                                                                                                                                 <  7:211198/119‑1 (MQ=255)
                                             aCTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTc                                                                                                                                 >  8:211198/1‑119 (MQ=255)
                                                   ttGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCAACCAGCAGCTCGACCGGCTGGCCAAACtt                                                                                             >  1:73271/1‑149 (MQ=255)
                                                     gatgaAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTg                                                                                            <  8:40133/148‑1 (MQ=255)
                                                     gatgaAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTg                                                                                            <  7:150045/148‑1 (MQ=255)
                                                         aaGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGGGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGt                                                                                        <  7:134846/148‑1 (MQ=255)
                                                          aGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGccacca                                                                                                                      >  4:153390/1‑117 (MQ=255)
                                                          aGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGccacca                                                                                                                      <  3:153390/117‑1 (MQ=255)
                                                              cTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCGTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCg                                                                                  >  6:324226/1‑149 (MQ=255)
                                                                gctcGGGCATGCACGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTg                                                                                            >  1:323442/2‑137 (MQ=255)
                                                                gctcGGGCATGCACGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTg                                                                                            <  2:323442/136‑1 (MQ=255)
                                                                 ctcGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCggg                                                                                <  5:229934/148‑1 (MQ=255)
                                                                    gTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGATTCGCCGGCCACCAGCAGCTCGACCGCCTGGCCAACCATGTAGTCGGCCGGGCTGa                                                                            >  2:257517/1‑149 (MQ=255)
                                                                        aTGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATc                                                                        >  6:112678/1‑149 (MQ=255)
                                                                            ccGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGccac                                                                                                                        <  3:93070/97‑1 (MQ=255)
                                                                            ccGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGccac                                                                                                                        >  4:93070/1‑97 (MQ=255)
                                                                            ccGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAgg                                                                    >  1:113361/1‑149 (MQ=255)
                                                                            ccGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAgg                                                                    >  6:123053/1‑149 (MQ=255)
                                                                              gAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTa                                                                  <  5:112678/149‑1 (MQ=255)
                                                                                     aGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTc                                                            >  3:316261/1‑148 (MQ=255)
                                                                                      gAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTc                                                            >  5:325080/1‑147 (MQ=255)
                                                                                        aCTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAg                                                         <  6:264091/148‑1 (MQ=255)
                                                                                          ttGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGgcg                                                      >  1:250771/1‑149 (MQ=255)
                                                                                            gAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGgcg                                                      <  2:250771/147‑1 (MQ=255)
                                                                                                acCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGt                                                >  4:172095/1‑149 (MQ=255)
                                                                                                  cTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGTCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTgg                                              >  6:172183/1‑149 (MQ=255)
                                                                                                  cTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGgc                                                       <  8:168813/140‑1 (MQ=255)
                                                                                                  cTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGgc                                                       >  7:168813/1‑140 (MQ=255)
                                                                                                  cTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTgg                                              <  7:27238/149‑1 (MQ=255)
                                                                                                  cTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTgg                                              >  7:217492/1‑149 (MQ=255)
                                                                                                    cGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCa                                            <  5:123053/149‑1 (MQ=255)
                                                                                                           gtgGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGa                                        <  4:316261/146‑1 (MQ=255)
                                                                                                              gATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGTCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCcggc                                  <  5:172183/149‑1 (MQ=255)
                                                                                                                  aGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCggg                               <  8:224783/148‑1 (MQ=255)
                                                                                                                     ccccAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTgcg                           >  3:212390/1‑149 (MQ=255)
                                                                                                                     ccccAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTgcg                           >  4:85319/1‑149 (MQ=255)
                                                                                                                       ccAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCt                         >  5:29199/1‑149 (MQ=255)
                                                                                                                          gTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGt                      <  4:53753/149‑1 (MQ=255)
                                                                                                                             gCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGa                   <  6:29199/149‑1 (MQ=255)
                                                                                                                              cGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCTGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAg                  <  6:77107/149‑1 (MQ=255)
                                                                                                                                gttgttGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCAACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAg                                                                     <  1:36476/96‑1 (MQ=255)
                                                                                                                                gttgttGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCAACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAg                                                                     >  2:36476/1‑96 (MQ=255)
                                                                                                                                  tgttgATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTa               >  5:140794/1‑148 (MQ=255)
                                                                                                                                   gttgATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTaca             >  8:256858/1‑149 (MQ=255)
                                                                                                                                       ccgatgGGCTTCAAGGCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTg         >  1:194390/3‑149 (MQ=255)
                                                                                                                                       ccgatgGGCTTCAAGGCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTg         >  1:189656/3‑149 (MQ=255)
                                                                                                                                        tgatgGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACTTCGAGGTCGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGc        <  1:257517/149‑1 (MQ=255)
                                                                                                                                         gatgGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGca       >  1:309463/1‑149 (MQ=255)
                                                                                                                                         gatgGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGc        <  8:265340/148‑1 (MQ=255)
                                                                                                                                         gatgGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGACGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGca       >  4:222996/1‑149 (MQ=255)
                                                                                                                                              ccTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGCACCACg  >  1:9366/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCGCCTACCGGCTGCAGGCTCAGTGAAATCTTGCCGTCCTGGCGCACTTCCTTGATGAAGCCCTTCTCGTGCATGCCCGAGCGCAAGAACTTGAACACCTCGTTCTTGTGGATCAGGCCCCAGTGGCGGTTGTTGATGATGGCCTTGAAGCCCATCGGCGTTTCGCCGGCCACCAGCAGCTCGACCGGCTGGCCAACCTTGTAGTCGGCCGGGGTGACATCGAGGTAGCGGTCCAGGCGCGACGTGGCAGTGATCCGGCGGGTGCGCTTGTCGAGGTACACGTGCACCACG  >  NC_002947/5183099‑5183389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑