| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | 100% | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 100.0% | 74.5 / NA | 26 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (13/13); total (13/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 100.0% | 74.8 / NA | 26 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (13/13); total (13/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCT > NC_002947/1499344‑1499618 || ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCg < 5:132115/149‑1 (MQ=255) aGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGttc < 1:73595/149‑2 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCATAGCCTTAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCACGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg < 1:217831/149‑6 (MQ=37) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAc‑g‑cccgc < 5:256675/143‑4 (MQ=25) ggCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTCCCGCGAAACAGGCGC‑CGCCGTGCATGGCACCCGCTTTGCCGGTGTTAGCGGGCAC‑GCCCGCTCCcacacccagat > 3:50934/1‑142 (MQ=255) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACAC‑CCGCTCCCACAGACTGATTGCCATTAAGc > 5:293405/1‑149 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACAC‑CCGCTCCCACAGACTGATTGCCATTAAGCgg < 6:293405/149‑1 (MQ=21) ggCACCACAGCAGTACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGGGC‑CGCGGTGCATGGCACCGGCTTTGCGGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa < 6:131943/148‑1 (MQ=21) cggCTGCCAAACGGTGGGAGCGGGCGGGCCCGCTAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg < 5:8511/147‑1 (MQ=21) aGGCGGCCAAACGGTGGGCGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAG‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg < 7:250556/148‑1 (MQ=21) gCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 6:140587/149‑1 (MQ=25) cccGCGAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 6:286995/1‑148 (MQ=37) ccGCGAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt > 8:141133/1‑139 (MQ=25) ccGCGAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 7:141133/139‑1 (MQ=25) ccGCGAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 5:308229/1‑147 (MQ=37) ccGCGAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 3:311949/1‑147 (MQ=37) cgcgAAACAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 6:253857/1‑146 (MQ=37) cAGGCGC‑CGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt > 5:220855/1‑148 (MQ=37) gc‑cgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 2:11016/1‑146 (MQ=37) gc‑cgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCa < 5:82216/147‑1 (MQ=37) gc‑cgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCa > 6:82216/1‑147 (MQ=37) c‑cgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc < 4:311949/149‑1 (MQ=37) cgcgGTGCCGGGCACCGGCTTTGCCGGTGTTCGCGGGCCC‑GCCCGCGCCAACAGACGGATTGCCATTAAGCGGGCCTATCGCGACAGGGCCGGCCCGTGTTGTGTTGCCTCGCTCACGACTCCTGACGACCGTAGCACGCGATCAAg > 2:293924/1‑147 (MQ=12) cgcgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt > 4:99310/1‑106 (MQ=21) cgcgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 3:99310/106‑1 (MQ=21) gCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCt > 2:109991/1‑148 (MQ=38) || GGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGC‑CGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCT > NC_002947/1499344‑1499618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |