Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	195,501	Δ1 bp	coding (1007/26049 nt)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	195,498	0	C	.	100.0%	74.6 / NA	25	coding (1004/26049 nt)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (13/12);  total (13/12)

AAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAC  >  NC_002947/195358‑195642                                                                                                                                             |                                                                                                                                                 aaGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCaa                                                                                                                                         >  8:111713/1‑149 (MQ=255)                  gCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACaacaac                                                                                                                         <  6:285788/148‑1 (MQ=37)                  gCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACg                                                                                                                        <  1:324953/149‑1 (MQ=37)                      gATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTc                                                                                                                    >  5:293496/1‑149 (MQ=25)                       aTCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCAGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTc                                                                                                                    <  7:176903/148‑1 (MQ=19)                        tCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGa                                                                                                                  <  3:42248/149‑1 (MQ=21)                            cTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGt                                                                                                              <  3:162292/149‑1 (MQ=21)                                acCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGc                                                                                                          <  7:111713/149‑1 (MQ=21)                                  cGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGt                                                                                                        >  3:97980/1‑149 (MQ=21)                                        ccTGAGCAACCCTGCCCAGACGCTGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACATCTACAACAACGGTTCGCCCGTCAGCGTCACCAt                                                                                                  >  7:177768/1‑149 (MQ=255)                                        ccTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGAc                                                                                                                                     >  5:302219/1‑114 (MQ=25)                                        ccTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGAc                                                                                                                                     <  6:302219/114‑1 (MQ=25)                                        ccTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACCGTTCGACCGTCAGCGTCACCAt                                                                                                  >  4:102559/1‑149 (MQ=19)                                           gAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATc                                                                                                 >  1:220376/1‑147 (MQ=21)                                                  ccTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGcc                                                                                        >  8:355041/1‑149 (MQ=17)                                                      cccAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACACCAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACcg                                                                                    >  7:351811/1‑149 (MQ=12)                                                                    accgtgaccCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg                                                                      <  7:355041/149‑1 (MQ=25)                                                                     ccgtgaccCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGc                                                                     <  6:361629/149‑1 (MQ=25)                                                                                       gCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGa                                                   <  2:220376/149‑1 (MQ=255)                                                                                                              ggCAAAACCCAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAc                            >  2:236801/1‑149 (MQ=37)                                                                                                                      ccAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCCGCCCCCGAt                                >  6:349920/1‑137 (MQ=11)                                                                                                                      ccAGGGCAGCGTCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGAt                                <  5:349920/137‑1 (MQ=21)                                                                                                                              gCGTCTATTTCCAGA‑CCCTGACAATGACATCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAcacca            <  8:177768/149‑1 (MQ=11)                                                                                                                                 tCGATTTCCAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAcaccacca         >  5:89839/1‑149 (MQ=255)                                                                                                                                        ccAGA‑CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAc  >  7:10991/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                 AAGCGTGACCGAAGGTGGCGTGATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAC  >  NC_002947/195358‑195642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑