Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,499,508	+C	intergenic (+85/‑79)	trpS → / → zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,499,506	1	.	C	100.0%	65.0 / NA	23	intergenic (+83/‑81)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (9/14);  total (9/14)

AGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCG  >  NC_002947/1499386‑1499646                                                                                                                            |                                                                                                                                             agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAcg‑cccgct                                                                                                                                        <  8:263359/128‑5 (MQ=21) agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAcg‑cccgct                                                                                                                                        >  7:263359/1‑124 (MQ=21)   agtcTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAg                                                                                                                  >  5:175717/4‑149 (MQ=21)       tGAACGACGCCTTGGCACCACAGCAGCACTGAGGCTGCCAAACGGTGCGAGCGGGCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCcac                                                                                                                                    <  5:112067/127‑1 (MQ=18)              aGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg                                                                                                       >  5:355443/1‑149 (MQ=21)                       accacAGCATGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa                                                                                              <  1:113603/149‑1 (MQ=21)                                ggACTGAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtt                                                                                     <  7:185432/149‑1 (MQ=21)                                      aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg                                                                                <  6:175717/148‑1 (MQ=21)                                        gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGGAAAAGGCGCCGCCGGTG‑ATGGCACCGGCATTTCCCGTGTTAGCGGGCAAGCCCGCGCCCACACACTGATTGCCAATAAACGGGCCGATCGCTACAGTGCAGGACCGGATTttgttg                                                                             >  8:252056/1‑149 (MQ=12)                                        gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                              <  4:101604/148‑1 (MQ=21)                                                         gCGGGCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCGGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                            <  8:323226/149‑1 (MQ=25)                                                           gggCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                                                          <  2:73565/149‑1 (MQ=37)                                                           gggCGTGCCCGCGAAACAGGCGCCG‑CGGTGCAGGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                                                          <  3:219187/149‑1 (MQ=37)                                                                   ccGCGAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                  <  1:20421/149‑1 (MQ=37)                                                                    cgcgAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                                    >  1:35722/1‑146 (MQ=37)                                                                       gAAACAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc                                              >  6:125025/1‑149 (MQ=37)                                                                           cAGGCGCCG‑CGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa                                          <  6:355443/149‑1 (MQ=37)                                                                                  cg‑cgGTGCATGGCACCGGCTTTGCCGGTGTTCGCTGTCACGCCCTCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCa                                   <  7:252056/149‑1 (MQ=37)                                                                                  cg‑cgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg                                     >  6:227761/1‑147 (MQ=37)                                                                                                             gtgttcgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa         >  7:255250/8‑149 (MQ=255)                                                                                                              tgttcgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt        <  2:323587/143‑1 (MQ=255)                                                                                                               tttcgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTAAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATg       <  4:283237/144‑1 (MQ=255)                                                                                                                   gcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc                                    >  2:96371/2‑116 (MQ=255)                                                                                                                   gcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc                                    <  1:96371/115‑1 (MQ=255)                                                                                                                    cGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgcg  >  4:74290/1‑149 (MQ=255)                                                                                                                            |                                                                                                                                             AGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCG‑CGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCG  >  NC_002947/1499386‑1499646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑