Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	3,845,705	+G	intergenic (‑72/+26)	PP_3394 ← / ← PP_3395	3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,845,703	1	.	G	100.0%	115.0 / NA	35	intergenic (‑70/+28)	PP_3394/PP_3395	3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/13);  total (22/13)

TGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAA  >  NC_002947/3845559‑3845840                                                                                                                                                  |                                                                                                                                           tgctgTCAGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑gggc                                                                                                                                        >  2:237778/1‑147 (MQ=255)   ctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTcgc                                                                                                                                             <  5:166653/143‑1 (MQ=255)   ctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTcgc                                                                                                                                             >  6:166653/1‑143 (MQ=255)    tgTCGGCATGATCAGTTGGAGGGTCTGCAAGCCATGGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGGTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑gggctga                                                                                                                                     <  1:285910/149‑6 (MQ=255)      tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCTGGCCGAGTTCTTTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑gggc                                                                                                                                        <  7:310666/144‑3 (MQ=255)      tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATCACGGGCAGAAAATCCGTGTTTCGC‑gggc                                                                                                                                        >  8:310666/1‑142 (MQ=255)             gATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGt                                                                                                                             >  2:19854/1‑148 (MQ=255)              aTCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGt                                                                                                                             >  7:303764/1‑147 (MQ=255)                  cTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCa                                                                                                                       >  6:118871/1‑149 (MQ=255)                  cTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCAAGACTAAGAGCTGGATTACTGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCATCCa                                                                                                                       >  8:326418/1‑149 (MQ=255)                     gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGa                                                                                                                    >  3:212964/1‑149 (MQ=255)                      cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGAt                                                                                                                   <  4:220760/149‑1 (MQ=255)                        ggCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATg                                                                                                                  >  3:110111/1‑148 (MQ=255)                          ctctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGTGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGAt                                                                                                               <  4:186556/149‑1 (MQ=255)                           tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGAt                                                                                                               >  1:218637/1‑148 (MQ=255)                               cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGcc                                                                                                          >  6:66353/1‑149 (MQ=255)                               cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGc                                                                                                           <  1:19854/148‑1 (MQ=255)                               cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGc                                                                                                           <  4:110111/148‑1 (MQ=255)                                        gcgcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCa                                                                                                             <  4:109374/137‑1 (MQ=255)                                        gcgcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCa                                                                                                             >  3:109374/1‑137 (MQ=255)                                         cgcCGGCCTAATTCATTTATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TTATCCATCGTCAGCCACGATGTATCAGCCCGCGCCCTGc                                                                                                <  6:375271/149‑1 (MQ=255)                                                        ttgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACg                                                                                  >  5:360431/1‑148 (MQ=255)                                                           attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcag                                                                               >  7:305258/1‑148 (MQ=255)                                                             tgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCaa                                                                            >  6:217021/1‑149 (MQ=255)                                                                 attgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg                                                                        >  1:176428/1‑149 (MQ=255)                                                                    gttgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtg                                                                      <  2:218637/148‑1 (MQ=255)                                                                       gtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTc                                                                   >  7:61860/1‑148 (MQ=255)                                                                          aTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TTATCCATCGTCTGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAg                                                                <  1:237778/148‑1 (MQ=255)                                                                                     tctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCCCGATGGATAAGCCCGCGCCCAGCCACGCCCGTGCACCCACCAACAGGTGGTCAACCAGGCgccccgc                                                    >  5:31754/1‑145 (MQ=255)                                                                                             aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCCTCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCg                                            <  5:66353/149‑1 (MQ=255)                                                                                                      gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtgt                                   >  6:180391/1‑149 (MQ=255)                                                                                                      gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATACATCGTGAGCCACGATGGATAAACCCCCGCCCAGCCAGGCCGGTGCACGCCGCAACAGGTGGTCAAGCAGGGCCCGACCCGCCCCCGAGAGccggt                                   >  4:19279/1‑145 (MQ=255)                                                                                                       ccAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGtgtg                                  >  1:283330/1‑149 (MQ=255)                                                                                                         aGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGcc                                <  8:61860/149‑1 (MQ=255)                                                                                                             tAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGACCGCGCCCTGCAAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCAGGTc                            >  5:343755/1‑149 (MQ=255)                                                                                                               agagCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTc                            <  6:360431/147‑1 (MQ=255)                                                                                                                          aCGGGCAGAAAAGACGTGTTTCGCGGGCATGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcag                                                                               >  1:21323/1‑86 (MQ=255)                                                                                                                              gCAGAAAAGACGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACg            >  1:255877/1‑148 (MQ=255)                                                                                                                                  aaaccaCGTGTTTCGCGGGC‑TGATCCATCGCCAGCCACTATGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTc       <  5:118871/144‑1 (MQ=255)                                                                                                                                       aCGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCAcc    >  6:334739/1‑147 (MQ=255)                                                                                                                                       aCGTGTTTCGCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCaa  <  5:334739/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                           TGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAA  >  NC_002947/3845559‑3845840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑