Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,027,334	A→C	T42P (ACC→CCC)	wbpL →	glycosyl transferase WbpL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,027,334	0	A	C	100.0%	139.9 / NA	43	T42P (ACC→CCC)	wbpL	glycosyl transferase WbpL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/21);  total (22/21)

TTCTGGGTGCACGCCGCCCATGATATGGCTGATTGTGCTGGCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCAACCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTACGTTGCTCCGGCAGTGCTGACCAGTGTGTTGGGGG  >  NC_002947/2027192‑2027442                                                                                                                                               |                                                                                                             ttCTGGGTGCACGCCGCCCATGATATGGCTGATTGTGCTGGCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCAccc                                                                                                            <  2:374086/145‑1 (MQ=255)        tGCACGCCGCCCATGATATGGCTGATTGTGCTGGCAACTGTAGCCTCGTGCTTCATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCACAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAggcgg                                                                                                 >  3:298815/1‑149 (MQ=255)         gCACGCCGCCCATGATATGGCTGATTGTGCTGGCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAggcggc                                                                                                >  1:147247/1‑149 (MQ=255)           aCGCCGCCCATGATATGGCTGATTGTGCTGGCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAggcggcgg                                                                                              >  2:98576/1‑149 (MQ=255)                    aTGATATGGCTGATTGTGCTGGCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCggcttggct                                                                                       >  1:230745/1‑147 (MQ=255)                    aTGATATGGCTGATTGTGCTGGCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCggcttggct                                                                                       <  2:230745/147‑1 (MQ=255)                        tatGGCTGATTGTGCTGGCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTg                                                                                 <  3:100230/149‑1 (MQ=255)                          tGGCTGATTGTGCTGGCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGt                                                                               >  2:32511/1‑149 (MQ=255)                          tGGCTGATTGTGCTGGCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGt                                                                               >  2:82697/1‑149 (MQ=255)                                aTTGTGCTGGCAACTGTAGCCTCGTGCTTGATGACAGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCttt                                                                         >  8:238757/1‑149 (MQ=255)                                    tgCTGGCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCt                                                                      <  3:243280/148‑1 (MQ=255)                                      cTGGCATCTGTAGCGTCGTGCTTGATGAGCGCGGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCTATGCGCGTAGCTCGCATTCCTTGCGACCCGCTCGAGGCGGCGGCTTGGCTTTCGTGGTGGCTTTCCTGGc                                                                   <  4:298815/149‑1 (MQ=255)                                      cTGGCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGc                                                                   >  2:43628/1‑149 (MQ=255)                                         gCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGc                                                                >  5:35736/1‑149 (MQ=255)                                           aaCTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCggcttgg                                                                                         <  6:250611/122‑1 (MQ=255)                                           aaCTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCggcttgg                                                                                         >  5:250611/1‑122 (MQ=255)                                              tGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGc                                                           >  7:198027/1‑149 (MQ=255)                                                tAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCt                                                          <  1:82697/148‑1 (MQ=255)                                                tAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTg                                                         >  4:147092/1‑149 (MQ=255)                                                tAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTg                                                         <  6:169561/149‑1 (MQ=255)                                                          cTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCtgggg                                                <  7:238757/148‑1 (MQ=255)                                                              atgaCCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCtggggtggg                                            <  7:252118/148‑1 (MQ=255)                                                                 aCCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCAGGCTGCGTTGCTGGGCCTGGGGTGGGGCgg                                        <  1:32511/149‑1 (MQ=255)                                                                  ccgccgTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCggg                                       >  7:284574/1‑149 (MQ=255)                                                                   cgccgTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGt                                      <  1:43628/149‑1 (MQ=255)                                                                   cgccgTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCACCGCGAGGCGGCGGCTTGCCTTTCGTGCAAGCTTACCTGGCTGCGTTGCTGGCACTGGGGAGCGGCggg                                       >  8:264770/1‑148 (MQ=255)                                                                    gctgTGCGGCGCCGCTATGCGCGGTCCCGTACCCTGTTGGACATCCCCAATGCGCTTATCTCGCATACCTTGCCACCCCCGCGAGGCGGGGGGTTGGCTTTCGTGTTCGCTTTCCTGGCTGCGGTGCTGGGCCTGGGGGGGGGCgggaa                                     <  2:349583/146‑3 (MQ=255)                                                                    gccgTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTa                                     <  1:98576/149‑1 (MQ=255)                                                                              gccgcTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGTGCGGGTACGTTGCTCCg                           >  5:17243/1‑149 (MQ=255)                                                                                  cTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTACGTTGCTCCGGCAg                       >  7:45169/1‑149 (MQ=255)                                                                                     tGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTACGTTGCTCCGGCAGt                      <  3:147092/147‑1 (MQ=255)                                                                                         cggTCCCGTCGCCTGTGGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGCGGCGGCGGCTTGGCTTTCGGGGTCGCTTTCCTGGCTGCGTTGCTGGGCCGGGGGTGGGGCGGGTACGTTGCTCCGGCAGTGCTGAc                <  8:284574/147‑1 (MQ=255)                                                                                         cTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTACGTTGCTCCGGCAGTGCTGAc                <  8:198027/149‑1 (MQ=255)                                                                                            tCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTACGTTGCTCCGGCAGTGCTGACCAg             >  3:261983/1‑149 (MQ=255)                                                                                              ccGTAGCCTGGTGGACAGGCCCAATGCGCGTTGCTCGCATTCCTTGCCACGCCCGGGCGGCGGCGGCTTGGGTTTCGTGGTCTGTTTCCTGGCTGGGTTGCTGGGCGTGGGGTGGGGCGGGTACGTTGCTCCGGCAGTGCTGACCAgt            <  7:264770/148‑1 (MQ=255)                                                                                                   gCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTACGTTGCTCCGGCAGTGCTGACCAGTGTGtt       <  4:261983/148‑1 (MQ=255)                                                                                                    ccTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCtggggtggg                                            <  5:222767/110‑1 (MQ=255)                                                                                                    ccTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCtggggtggg                                            >  6:222767/1‑110 (MQ=255)                                                                                                    ccTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTACGTTGCTCCGGCAGTGCTGACCAGTGTGTTgg     >  7:274794/1‑149 (MQ=255)                                                                                                                              gCTCGCATACCTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTa                                     >  3:2947/1‑91 (MQ=255)                                                                                                                                        caagCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTACGTTGCTCCGGCAGTGCTGACCAGTGTGTTggggg  >  3:98007/4‑116 (MQ=255)                                                                                                                                        caagCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGGGGGGCGGGTACGTTGCTCCGGCAGTGCTGACCAGTGTGTTggggg  <  4:98007/113‑1 (MQ=255)                                                                                                                                        cTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTACGTTGCTc                             >  6:352751/1‑89 (MQ=255)                                                                                                                                        cTTGCCACCCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTACGTTGCTc                             <  5:352751/89‑1 (MQ=255)                                                                                                                                               |                                                                                                             TTCTGGGTGCACGCCGCCCATGATATGGCTGATTGTGCTGGCAACTGTAGCCTCGTGCTTGATGACCGCCGTGCTGCGCCGCTATGCGCTGTCCCGTAGCCTGTTGGACATCCCCAATGCGCGTAGCTCGCATACCTTGCCAACCCCGCGAGGCGGCGGCTTGGCTTTCGTGGTCGCTTTCCTGGCTGCGTTGCTGGGCCTGGGGTGGGGCGGGTACGTTGCTCCGGCAGTGCTGACCAGTGTGTTGGGGG  >  NC_002947/2027192‑2027442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑