Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,126,647	+C	intergenic (‑101/+48)	tdcG‑II ← / ← gcvP‑I	L‑serine dehydratase/glycine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,126,645	1	.	C	100.0%	60.1 / NA	19	intergenic (‑99/+50)	tdcG‑II/gcvP‑I	L‑serine dehydratase/glycine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (10/9);  total (10/9)

GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCC  >  NC_002947/1126500‑1126768                                                                                                                                                   |                                                                                                                            gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCGCGAATGCGGGGGTGTATCCACCATAGACTTCGCGGGTAAA‑ccc                                                                                                                          >  4:297739/1‑148 (MQ=255) gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑cc                                                                                                                           <  2:82062/148‑1 (MQ=255)    gggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑cccgct                                                                                                                       <  3:230328/149‑5 (MQ=255)          atgccgatCTTGAACAGGTCGAAGACGCTCAGTGACTTGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGTTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTaca                                                                                                                  <  8:191394/149‑1 (MQ=255)                                            aCATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACACCCGCATTCGGAGGTTTAGCCACCATAGCATTAGCGGGTAAACCCCCACCTACACAGAGCCCGCCCCCCAACCCAGGTGGCGGGGAgg                                                                                >  6:300107/1‑149 (MQ=255)                                             cATGTGGACCTCCTGCCAGGTTGTTTGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGATTGCGGTGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAggg                                                                               <  5:104912/149‑1 (MQ=38)                                                   gACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTTACCCCGAAAGCGGAGGTATAGCCACCATAGCAATAGCCGGTAAACCCCCACCTACACAGAGCCCCCCACCCCAGCCCGGTGGCGGGGAGGGCTAt                                                                           >  2:51512/1‑147 (MQ=255)                                                    tcctcctGCCAGGTTTGTGGGTGGACTTTGCGCCCTGAAGGCGCGTGTTTCCCGGCGATTGCGGTGGTATAGCCACCTTCGCATTGGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg                                                                        <  4:257530/148‑1 (MQ=255)                                                                                                   tttACCCGCTAATGCGGAGGTATAGCCACCATCGCATTCGCGGTTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCg                         <  3:377867/149‑1 (MQ=255)                                                                                                   tttACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCg                         <  5:300107/149‑1 (MQ=255)                                                                                                       cccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTTGATCGACGGGCAGGCGCACACCAGGTTGCGGTc                      <  1:51512/148‑1 (MQ=255)                                                                                                        ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc                    >  8:11814/1‑149 (MQ=255)                                                                                                        ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGACCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc                    >  3:323489/1‑149 (MQ=255)                                                                                                         cgcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc                   >  4:227486/1‑149 (MQ=255)                                                                                                          gcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGATGCGGTCGcc                   >  4:164689/1‑148 (MQ=255)                                                                                                           cgAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCg                  >  7:77299/1‑148 (MQ=255)                                                                                                                  ggAGGTATAGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCGGTCGATCGACGGGCAGGCGCACACAAGGTTGCGGTCGCCGAACACGtt          >  8:161629/1‑149 (MQ=39)                                                                                                                     ggTATAGCCACAATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTc       <  3:15442/149‑1 (MQ=255)                                                                                                                       tataGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGa     <  4:323489/149‑1 (MQ=255)                                                                                                                        ataGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCt                                                                 >  4:94303/1‑88 (MQ=255)                                                                                                                        ataGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCt                                                                 <  3:94303/88‑1 (MQ=255)                                                                                                                          aGCCACCATCGCATTCGCGGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAccc  >  6:42673/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                            GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCC  >  NC_002947/1126500‑1126768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑