Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,499,498 1 bp→CG intergenic (+75/‑89) trpS → / → zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,499,4971.C93.9% 142.0 / ‑3.8 50intergenic (+74/‑90)trpS/zapEtryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  major base C (19/27);  minor base A (3/0);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 8.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0029471,499,4980TG98.0% 146.7 / ‑5.8 50intergenic (+75/‑89)trpS/zapEtryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (21/28);  minor base A (1/0);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGG‑CACG‑CCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCA  >  NC_002947/1499361‑1499639
                                                                                                                                           ||                                                                                                                                               
gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CAcg‑cc                                                                                                                                      >  6:179437/1‑149 (MQ=37)
          gttaTGCGGCCATTCAGAGCCTTACCGAAGCCTTGCCACCACAGCAGGAGTGAGGCTGCCAAACGGTGGGAGGGGGCGTGCCCGCGAAACTGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCc‑aca                                                                                                                            <  8:105391/146‑1 (MQ=18)
          gTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCc‑a                                                                                                                              >  2:66192/1‑147 (MQ=25)
                         agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCAtt                                                                                                              <  8:304972/148‑1 (MQ=21)
                              cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCgg                                                                                                        <  3:218303/149‑1 (MQ=21)
                                    gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCa                                                                                                                >  5:181071/1‑135 (MQ=21)
                                    gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCa                                                                                                                <  6:181071/135‑1 (MQ=21)
                                      aGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCg                                                                                                <  1:66192/149‑1 (MQ=21)
                                        ccTTGGCACCACAGCATGTCTGAGGCTTCCAAACGGTGGGAGCTGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGATTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCt                                                                                              <  5:155262/149‑1 (MQ=21)
                                          ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTa                                                                                             >  5:350225/1‑148 (MQ=21)
                                              caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTg                                                                                        <  1:232526/149‑1 (MQ=21)
                                                 cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCCTTCCCGCGAAACAAGCGCCGCGGTGCATGGCACCCGCTTTGCCGGTGT‑TAGCGGG‑GACGCCCGCTCCC‑CCAGACAGATTTCCAATAAGCCGGCCTATCGCGACAGTGACg                                                                                     >  6:352125/1‑149 (MQ=255)
                                                     gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCCTGCCCGCCAAACACGCCCCCCGGTGCAAGGCACCCGCATTGCCGGTGT‑TAACGGGCCCCC‑CCCCTCCC‑ACAGACAGAAAGGCAATAAACGGGCCGAAAGCAACAATGAccggcc                                                                                 >  2:107054/1‑144 (MQ=255)
                                                       agGACTGAGGCTGCCTATCGGGGGGAGCGGGCGTGCCGGCGAAACTGGCGCCGCGGTGCATGGCACCGGCTTGGCCTGTGT‑TCGGGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcgcgg                                                                               <  4:329850/149‑2 (MQ=21)
                                                          aCTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCCCCGTGCATTGCACCCGCTTTGCCGGTGT‑TAGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCAATAAGCGGGCCGATCGCAAAAGTGTCGGCCCGgttt                                                                            >  8:293932/1‑149 (MQ=18)
                                                           cTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt                                                                           >  5:322882/1‑149 (MQ=21)
                                                              aGGCTGCCAACGGTTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGGGCATGGCACCGGCTTTGCCGGTTT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt                                                                          <  3:160093/147‑1 (MQ=21)
                                                                gCTGCCTAACGGTGGGAGGGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGGGGG‑CACGCCCGCTCCCTACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                       <  1:107054/149‑1 (MQ=21)
                                                                           gTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCAGGGCACCGGCTTTGCCGGTGTGC‑GCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACg                                                           <  4:58937/149‑1 (MQ=21)
                                                                                       gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                                               <  6:322882/149‑1 (MQ=37)
                                                                                           ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                             >  7:92093/1‑147 (MQ=37)
                                                                                           ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                             >  2:223838/1‑147 (MQ=37)
                                                                                            cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                             >  4:305147/1‑146 (MQ=37)
                                                                                             gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                                               >  5:288427/1‑143 (MQ=37)
                                                                                             gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                                               <  6:288427/143‑1 (MQ=37)
                                                                                                  aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt                                    <  6:350225/149‑1 (MQ=37)
                                                                                                   cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa                                                             >  8:192370/1‑123 (MQ=25)
                                                                                                   cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa                                                             <  7:192370/123‑1 (MQ=25)
                                                                                                     ggCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc                                 <  7:293932/149‑1 (MQ=37)
                                                                                                      gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAGTCATGACTCCCTTAGAACGCTATc                                 >  4:124735/1‑148 (MQ=37)
                                                                                                        gccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                           >  5:309455/1‑136 (MQ=37)
                                                                                                        gccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                           <  6:309455/136‑1 (MQ=37)
                                                                                                        gccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg                              >  8:314336/1‑149 (MQ=37)
                                                                                                         ccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAGTCATGACTCCCTTAGAACGCTATCAAGc                             <  3:124735/149‑1 (MQ=37)
                                                                                                          cgcgGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAACATGACTCCCTTAGAACGCTATCAAGCa                            <  1:223838/149‑1 (MQ=37)
                                                                                                                  aTGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGa                     >  6:9755/1‑148 (MQ=38)
                                                                                                                   tggcaccGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaaa                   >  5:168638/7‑149 (MQ=38)
                                                                                                                    ggcaccGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAAc                  >  7:6290/6‑149 (MQ=38)
                                                                                                                       accGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc               <  7:314336/147‑1 (MQ=38)
                                                                                                                        ccGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcc              <  8:6290/148‑1 (MQ=39)
                                                                                                                        ccGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcc              >  7:244503/2‑149 (MQ=39)
                                                                                                                        ccGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcc              >  6:101688/2‑149 (MQ=39)
                                                                                                                         cGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc             <  3:226507/149‑1 (MQ=39)
                                                                                                                            caaagCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc          <  5:281134/145‑1 (MQ=255)
                                                                                                                            caaagCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc          <  7:214302/145‑1 (MQ=255)
                                                                                                                             tttGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAAc                  <  5:204639/140‑1 (MQ=39)
                                                                                                                             tttGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAAc                  >  6:204639/1‑140 (MQ=39)
                                                                                                                               tGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActt        <  3:48602/148‑1 (MQ=255)
                                                                                                                                gCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtc       <  4:49889/148‑1 (MQ=255)
                                                                                                                                 ccGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtctt     <  6:168638/149‑1 (MQ=255)
                                                                                                                                     gtgttcgcGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTACCTTAGAACGCTATAAAGCAGATCTGAAACGTCCCGACTTCTTCCa  >  2:310096/8‑149 (MQ=255)
                                                                                                                                           gcGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                       <  7:305076/73‑1 (MQ=255)
                                                                                                                                           gcGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                       >  8:305076/2‑74 (MQ=255)
                                                                                                                                           ||                                                                                                                                               
GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGG‑CACG‑CCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCA  >  NC_002947/1499361‑1499639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑