Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,499,480	2 bp→AC	intergenic (+57/‑106)	trpS → / → zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,499,480	0	C	A	100.0%	121.4 / NA	41	intergenic (+57/‑107)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/23);  total (18/23)
*	NC_002947	1,499,481	0	A	C	100.0%	121.9 / NA	41	intergenic (+58/‑106)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/23);  total (18/23)

CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGG‑CACG‑CCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGA  >  NC_002947/1499339‑1499620                                                                                                                                               ||                                                                                                                                                 cGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTCGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCt                                                                                                                                             <  1:289191/149‑1 (MQ=255) cGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCtt                                                                                                                                            <  1:378407/149‑1 (MQ=255)                 gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt‑tcgcgg                                                                                                                            <  3:278333/148‑6 (MQ=255)                       gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CAcg‑cc                                                                                                                   >  6:179437/1‑149 (MQ=37)                                 gttaTGCGGCCATTCAGAGCCTTACCGAAGCCTTGCCACCACAGCAGGAGTGAGGCTGCCAAACGGT‑GGGAGGGGGCGTGCCCGCGAAACTGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCc‑aca                                                                                                         <  8:105391/146‑1 (MQ=18)                                 gTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCc‑a                                                                                                           >  2:66192/1‑147 (MQ=25)                                                agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCAtt                                                                                           <  8:304972/148‑1 (MQ=21)                                                     cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCgg                                                                                     <  3:218303/149‑1 (MQ=21)                                                      tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtg                                                                                                                                    >  3:7247/1‑104 (MQ=255)                                                      tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtg                                                                                                                                    <  4:7247/104‑1 (MQ=255)                                                           gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCa                                                                                             <  6:181071/135‑1 (MQ=21)                                                           gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCa                                                                                             >  5:181071/1‑135 (MQ=21)                                                             aGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCg                                                                             <  1:66192/149‑1 (MQ=21)                                                               ccTTGGCACCACAGCATGTCTGAGGCTTCCAAACGGT‑GGGAGCTGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGATTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCt                                                                           <  5:155262/149‑1 (MQ=21)                                                                 ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTa                                                                          >  5:350225/1‑148 (MQ=21)                                                                     caccacAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTg                                                                     <  1:232526/149‑1 (MQ=21)                                                                        cacaGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCCTTCCCGCGAAACAAGCGCCGCGGTGCATGGCACCCGCTTTGCCGGTGT‑TAGCGGG‑GACGCCCGCTCCC‑CCAGACAGATTTCCAATAAGCCGGCCTATCGCGACAGTGACg                                                                  >  6:352125/1‑149 (MQ=255)                                                                            gcagGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCCTGCCCGCCAAACACGCCCCCCGGTGCAAGGCACCCGCATTGCCGGTGT‑TAACGGGCCCCC‑CCCCTCCC‑ACAGACAGAAAGGCAATAAACGGGCCGAAAGCAACAATGAccggcc                                                              >  2:107054/1‑144 (MQ=255)                                                                              agGACTGAGGCTGCCTATCGGG‑GGGAGCGGGCGTGCCGGCGAAACTGGCGCCGCGGTGCATGGCACCGGCTTGGCCTGTGT‑TCGGGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcgcgg                                                            <  4:329850/149‑2 (MQ=21)                                                                                 aCTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCCCCGTGCATTGCACCCGCTTTGCCGGTGT‑TAGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCAATAAGCGGGCCGATCGCAAAAGTGTCGGCCCGgttt                                                         >  8:293932/1‑149 (MQ=18)                                                                                  cTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt                                                        >  5:322882/1‑149 (MQ=21)                                                                                     aGGCTGCCAACGGTT‑GGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGGGCATGGCACCGGCTTTGCCGGTTT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt                                                       <  3:160093/147‑1 (MQ=21)                                                                                       gCTGCCTAACGGT‑GGGAGGGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGGGGG‑CACGCCCGCTCCCTACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                    <  1:107054/149‑1 (MQ=21)                                                                                                  gT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCAGGGCACCGGCTTTGCCGGTGTGC‑GCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACg                                        <  4:58937/149‑1 (MQ=21)                                                                                                               gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                            <  6:322882/149‑1 (MQ=37)                                                                                                                   ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                          >  2:223838/1‑147 (MQ=37)                                                                                                                   ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                          >  7:92093/1‑147 (MQ=37)                                                                                                                    cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                          >  4:305147/1‑146 (MQ=37)                                                                                                                     gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                            >  5:288427/1‑143 (MQ=37)                                                                                                                     gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                            <  6:288427/143‑1 (MQ=37)                                                                                                                          aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt                 <  6:350225/149‑1 (MQ=37)                                                                                                                           cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa                                          <  7:192370/123‑1 (MQ=25)                                                                                                                           cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa                                          >  8:192370/1‑123 (MQ=25)                                                                                                                             ggCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc              <  7:293932/149‑1 (MQ=37)                                                                                                                              gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAGTCATGACTCCCTTAGAACGCTATc              >  4:124735/1‑148 (MQ=37)                                                                                                                                gccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                        >  5:309455/1‑136 (MQ=37)                                                                                                                                gccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                        <  6:309455/136‑1 (MQ=37)                                                                                                                                gccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg           >  8:314336/1‑149 (MQ=37)                                                                                                                                 ccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAGTCATGACTCCCTTAGAACGCTATCAAGc          <  3:124735/149‑1 (MQ=37)                                                                                                                                  cgcgGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAACATGACTCCCTTAGAACGCTATCAAGCa         <  1:223838/149‑1 (MQ=37)                                                                                                                                          aTGGCACCGGCTTTGCCGGTGT‑TCGCGGG‑CACGCCCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGa  >  6:9755/1‑148 (MQ=38)                                                                                                                                               ||                                                                                                                                                 CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGT‑GGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGG‑CACG‑CCGCTCCC‑ACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGA  >  NC_002947/1499339‑1499620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑