Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,229,191	Δ1 bp	11.5%	intergenic (‑133/+43)	PP_4606 ← / ← PP_4607	ferric siderophore receptor/transmembrane sensor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,229,191	0	G	.	11.5%	155.3 / 15.0	52	intergenic (‑133/+43)	PP_4606/PP_4607	ferric siderophore receptor/transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base G (26/20);  new base . (3/3);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

CTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCC  >  NC_002947/5229053‑5229338                                                                                                                                            |                                                                                                                                                     cTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg                                                                                                                                            >  2:158319/1‑149 (MQ=255)     aTGGACGCAGACCTGCGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTCTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑gg                                                                                                                                         <  1:58827/148‑1 (MQ=255)     aTGGACGCAGACCTGCGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑gg                                                                                                                                         >  2:58827/1‑147 (MQ=255)         aCGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑ggg                                                                                                                                        >  4:124031/1‑144 (MQ=255)         aCGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑ggg                                                                                                                                        <  3:124031/144‑1 (MQ=255)          cGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGc                                                                                                                                                     >  1:251022/1‑131 (MQ=255)          cGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGc                                                                                                                                                     <  2:251022/131‑1 (MQ=255)          cGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTAcc                                                                                                                                  <  7:94006/149‑1 (MQ=255)               aCCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGa                                                                                                                             <  3:236595/149‑1 (MQ=255)                    tgAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCGCGAagag                                                                                                                         <  2:174234/148‑1 (MQ=38)                              attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTAc                                                                                                                                   >  4:249632/1‑128 (MQ=255)                              attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTAc                                                                                                                                   <  3:249632/128‑1 (MQ=255)                              agtcttGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGGGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAg                                                                                                               <  5:83809/144‑1 (MQ=255)                               ttattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAgg                                                                                                              >  7:111144/1‑148 (MQ=255)                                     cGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTAc                                                                                                       <  8:279573/149‑1 (MQ=255)                                     cGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCGGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGGGAAGAGGCTGGCACAGGCAACTAc                                                                                                       <  5:265976/149‑1 (MQ=255)                                            atcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAAc                                                                                                  >  7:223474/1‑147 (MQ=255)                                                   tttCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTgg                                                                                                                    <  2:302594/122‑1 (MQ=255)                                                   tttCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACCGG‑GGGTTTACCCGTGAAGAGGCTgg                                                                                                                    >  1:302594/1‑122 (MQ=255)                                                         tCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGccc                                                                                   >  8:173655/1‑149 (MQ=255)                                                           aTTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTAAAGAGTCTGGCAAAGGCAACTACCGAACCAGGGTCAAACGcccc                                                                                  >  6:307839/1‑148 (MQ=255)                                                                  ggATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGt                                                                          >  8:64393/1‑149 (MQ=255)                                                                    tctccACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTc                                                                         <  3:67585/145‑1 (MQ=255)                                                                        cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAg                                                                                                               >  5:124282/1‑106 (MQ=255)                                                                        cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAg                                                                                                               <  6:124282/106‑1 (MQ=255)                                                                        cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTc                                                                         >  4:67585/1‑144 (MQ=255)                                                                        cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCg                                                                    >  4:202632/1‑149 (MQ=255)                                                                         aCGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGt                                                                   <  1:158319/149‑1 (MQ=255)                                                                               tGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGcac                                                                                                                 >  7:203291/1‑97 (MQ=255)                                                                               tGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGcac                                                                                                                 <  8:203291/97‑1 (MQ=255)                                                                                 ttACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAAcc                                                           <  3:202632/149‑1 (MQ=255)                                                                                            gtgCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGc                                                >  6:292323/1‑149 (MQ=255)                                                                                               ccTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGcc                                               >  5:160213/1‑147 (MQ=255)                                                                                                cTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAg                                            >  2:272044/1‑149 (MQ=255)                                                                                                  ggAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGc                                           >  5:38954/1‑148 (MQ=255)                                                                                                   gAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGc                                           <  5:307839/147‑1 (MQ=255)                                                                                                   gAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCg                                          >  7:61876/1‑148 (MQ=255)                                                                                                     aaTATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAAt                                       >  8:41513/1‑149 (MQ=255)                                                                                                            t‑tGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAgcg                                 <  6:160213/148‑1 (MQ=255)                                                                                                                cTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCcag                             <  6:172580/149‑1 (MQ=255)                                                                                                                        gcCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCt                     >  8:205441/1‑149 (MQ=255)                                                                                                                              tcacgGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCGCGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCa               >  1:194240/4‑149 (MQ=37)                                                                                                                                gcgGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCAcc             <  7:41513/149‑1 (MQ=255)                                                                                                                                acgGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCAcc             >  5:189684/2‑149 (MQ=38)                                                                                                                                 cgGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCg            <  5:292323/149‑1 (MQ=255)                                                                                                                                 cgGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCGCGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCg            <  2:194240/149‑1 (MQ=38)                                                                                                                                   ggTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCgg           >  3:108692/1‑148 (MQ=255)                                                                                                                                    gTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCggg          >  4:171979/1‑148 (MQ=255)                                                                                                                                    gTAAACCC‑CCCGTAGGGGCGGGTTTACCCGCGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCggg          >  2:38712/1‑148 (MQ=38)                                                                                                                                     tAAACCC‑CCCGTAGGGGCGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGaa        <  6:189684/149‑1 (MQ=39)                                                                                                                                        aCCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCt     >  1:3881/1‑149 (MQ=255)                                                                                                                                           cGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCCCCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGcc  >  6:33517/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                                     CTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTT‑TGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCC  >  NC_002947/5229053‑5229338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑