Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,264,299 Δ1 bp 10.2% intergenic (‑53/+31) zinU ← / ← PP_4640 P‑loop guanosine triphosphatase‑dependent zinc chaperone/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,264,2990A.10.2% 150.4 / 11.5 49intergenic (‑53/+31)zinU/PP_4640P‑loop guanosine triphosphatase‑dependent zinc chaperone/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/22);  new base . (2/3);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

GAGCTTCAGGCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCTGCATGTGTTCGACATAG  >  NC_002947/5264151‑5264447
                                                                                                                                                     |                                                                                                                                                     
gAGCTTCAGGCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCa                                                                                                                                                       <  8:31679/149‑1 (MQ=255)
 aGCTTCAGGCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAg                                                                                                                                                      <  1:208956/149‑1 (MQ=255)
      cAGGCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTc                                                                                                                                                  <  2:39620/148‑1 (MQ=255)
        ggCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGTCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCCTGGGTAACC‑GGCTCCca                                                                                                                                               <  8:65610/149‑1 (MQ=255)
        ggCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTccc                                                                                                                                                >  3:257641/1‑148 (MQ=255)
           cATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAg                                                                                                                                            <  1:206500/149‑1 (MQ=255)
           cATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAg                                                                                                                                            >  5:217844/1‑149 (MQ=255)
            aTGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAgg                                                                                                                                           >  4:114837/1‑149 (MQ=255)
                  ggCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTac‑a                                                                                                                                    >  6:198538/1‑149 (MQ=255)
                  ggCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGAACCTAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACTCAAAATACTCCGGAATCTGAAAATGACTTTGGGCGAC‑GGTTACCCAGGCTCCCACAGGTTTac‑a                                                                                                                                    >  6:237345/1‑149 (MQ=255)
                     cTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACg                                                                                                                                  >  3:183301/1‑148 (MQ=255)
                        gAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAAc                                                                                                                               <  3:114837/148‑1 (MQ=255)
                          gCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGc                                                                                                                            <  5:198538/149‑1 (MQ=255)
                           cATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCCTGGGTAACC‑GGCTCCCACAgtc                                                                                                                                          >  4:83531/1‑133 (MQ=39)
                           cATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCCTGGGTAACC‑GGCTCCCACAgtc                                                                                                                                          <  7:285276/135‑3 (MQ=39)
                           cATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCCTGGGTAACC‑GGCTCCCACAgtc                                                                                                                                          <  3:83531/135‑3 (MQ=39)
                           cATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCCTGGGTAACC‑GGCTCCCACAgtc                                                                                                                                          >  8:285276/1‑133 (MQ=39)
                            aTGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAg                                                                                                                          >  7:234450/1‑149 (MQ=255)
                                     aGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAg                                                                                                                 <  8:234450/149‑1 (MQ=255)
                                     aGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAg                                                                                                                 <  6:271834/149‑1 (MQ=255)
                                      gCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGc                                                                                                                <  3:59909/149‑1 (MQ=255)
                                                   gCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGa                                                                                                   >  3:96016/1‑149 (MQ=255)
                                                    ccGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGa                                                                                                   <  4:183301/148‑1 (MQ=255)
                                                    ccGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGa                                                                                                   <  6:217844/148‑1 (MQ=255)
                                                    ccGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGAc                                                                                                  <  7:281951/149‑1 (MQ=255)
                                                          tcGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCa                                                                                            <  5:211073/148‑1 (MQ=255)
                                                                 aGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAAc                                                                                     <  4:257641/149‑1 (MQ=255)
                                                                   ccTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGt                                                                                   >  5:202309/1‑149 (MQ=255)
                                                                     tgtgAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGc                                                                                 >  4:128200/1‑149 (MQ=255)
                                                                     tgtgAGGACGGTTAAGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCTCAGGCTCAACAGAACTTGGGCCCGGACTTGCCACCGTAACGTGc                                                                                 >  4:234114/1‑149 (MQ=255)
                                                                           gACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCt                                                                            >  6:102123/1‑148 (MQ=255)
                                                                             ctgTTACGTGAATGTGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGATCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGAc                                                                         <  3:37269/147‑1 (MQ=255)
                                                                                   cGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTACCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCTGACTTGCCACCGTAACGTGCTTCCTGACGTTcgc                                                                   <  4:96585/149‑1 (MQ=255)
                                                                                       aaTGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCgg                                                                 >  2:257031/1‑147 (MQ=255)
                                                                                        aTGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGgaag                                                              <  5:102123/149‑1 (MQ=255)
                                                                                                   cACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTa                                                   <  1:11941/149‑1 (MQ=255)
                                                                                                        aaaaTACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCtca                                              >  5:188049/1‑149 (MQ=255)
                                                                                                             aCTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCAcc                                         >  6:253503/1‑149 (MQ=255)
                                                                                                               tCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCgg                                       >  7:229361/1‑149 (MQ=255)
                                                                                                                    aaTCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGt                                  >  3:103108/1‑149 (MQ=255)
                                                                                                                      tCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTcc                                >  4:97124/1‑149 (MQ=255)
                                                                                                                      tCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTcc                                >  6:64208/1‑149 (MQ=255)
                                                                                                                      tCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTcc                                >  3:230806/1‑149 (MQ=255)
                                                                                                                          aaaaTGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGa                             <  3:128200/148‑1 (MQ=255)
                                                                                                                           aaaTGACTGTGGGAGCC‑GTTTACCCAGGCTTGCTCAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATg                           <  4:230806/149‑1 (MQ=255)
                                                                                                                                    tgGGAGCC‑GGTTACCCAGGCTCCCACAGGTGTAC‑ACGAACCGCACGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCTg                  <  5:226215/149‑1 (MQ=255)
                                                                                                                                      ggAGCC‑GGTTACCCAGGCTCCCACAGGTTTACGACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCTg                  >  1:192786/1‑148 (MQ=255)
                                                                                                                                             ggTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCTGCATGTGtt          <  5:64208/149‑1 (MQ=255)
                                                                                                                                                   ccAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAAt                                                           >  7:221631/1‑94 (MQ=255)
                                                                                                                                                   ccAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAAt                                                           <  8:221631/94‑1 (MQ=255)
                                                                                                                                                     aGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCGGGATGCGTTTTCTGCATGTGTTCGACATAg  <  4:96016/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAGCTTCAGGCCATGCTCGGCCTTGAGCATGTACTTGAGCAAGGTGGTCTTGCCGGCACCGAGGAAGCCTGTGAGGACGGTTACGGGAATGGGCATCTGCACGCAAAATACTCCTGAATCTGAAAATGACTGTGGGAGCC‑GGTTACCCAGGCTCCCACAGGTTTAC‑ACGAACCGCAGGCTCAACAGCACTTGGGCCCGGACTTGCCACCGTAACGTGCTTCCTGACGTTCGCGGAAGAATGCCTCGTAGCTCATCACCGGCTTGTCCGGATGCGTTTTCTGCATGTGTTCGACATAG  >  NC_002947/5264151‑5264447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑