Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,304,814	Δ1 bp	5.7%	coding (2438/3483 nt)	recC ←	multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,304,814	0	T	.	5.7%	225.8 / 6.4	70	coding (2438/3483 nt)	recC	multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit
Reads supporting (aligned to +/- strand):  ref base T (32/34);  new base . (2/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

CGCAAGAAGTCATTCAGCTGGTGCAGACTCAGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCA  >  NC_002947/5304673‑5304961                                                                                                                                              |                                                                                                                                                    cgcAAGAAGTCATTCAGCTGGTGCAGACTCAGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGaa                                                                                                                                              <  3:16203/149‑1 (MQ=255) cgcAAGAAGTCATTCAGCTGGTGCAGACTCAGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTCCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGaa                                                                                                                                              <  1:237869/149‑1 (MQ=255)    aagaagTCATTCAGCTGGTGCAGACTCAGGGCTTCACCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCGGGTGCAGCTCCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGgtagta                                                                                                                                           <  4:138401/149‑1 (MQ=255)            aTTCAGCTGGTGCAGACTCAGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGc                                                                                                                                   <  3:290287/149‑1 (MQ=255)             ttCAGCTGGTGCAGACTCAGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCt                                                                                                                                  >  5:126870/1‑149 (MQ=255)             ttCACCTGGTGCAGACTCAGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGc                                                                                                                                   >  2:273530/1‑148 (MQ=255)                             tCAGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCggg                                                                                                                  >  2:48559/1‑149 (MQ=255)                             tCAGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCC‑TCTGGAAGTACCGAggg                                                                                                                                    >  1:229488/1‑130 (MQ=255)                             tCAGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCC‑TCTGGAAGTACCGAggg                                                                                                                                    >  1:233451/1‑130 (MQ=255)                             tCAGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCC‑TCTGGAAGTACCGAggg                                                                                                                                    <  2:233451/130‑1 (MQ=255)                             tCAGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCC‑TCTGGAAGTACCGAggg                                                                                                                                    <  2:229488/130‑1 (MQ=255)                               aGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGGTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCggg                                                                                                                  <  4:56630/147‑1 (MQ=255)                               aGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAg                                                                                                                      >  8:87332/1‑143 (MQ=255)                               aGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAg                                                                                                                      <  7:87332/143‑1 (MQ=255)                               aGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTg                                                                                                                >  1:139293/1‑149 (MQ=255)                                 ggCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAggg                                                                                                                                    >  6:1018/1‑127 (MQ=255)                                 ggCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAggg                                                                                                                                    <  5:1018/127‑1 (MQ=255)                                 ggCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCt                                                                                                              >  5:243712/1‑149 (MQ=255)                                        gCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGt                                                                                                       <  2:211059/149‑1 (MQ=255)                                           tCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAg                                                                                                    >  3:189317/1‑149 (MQ=255)                                              tGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAgtgc                                                                                                 >  6:30972/1‑149 (MQ=255)                                                aTAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAgtgcgt                                                                                               <  1:47746/149‑1 (MQ=255)                                                    ggcggcAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCaa                                                                                           >  7:157967/1‑149 (MQ=255)                                                        gcAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGt                                                                                       >  5:75106/1‑149 (MQ=255)                                                           aGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGc                                                                                    >  8:250180/1‑149 (MQ=255)                                                            gCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCt                                                                                   <  6:243712/149‑1 (MQ=255)                                                              ccGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGATGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCg                                                                                 >  6:39670/1‑149 (MQ=255)                                                              ccGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCg                                                                                 >  1:112496/1‑149 (MQ=255)                                                               cGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACTTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTgg                                                                                                         <  8:155576/124‑1 (MQ=255)                                                               cGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACATGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTgg                                                                                                         >  7:155576/1‑124 (MQ=255)                                                                     gctcTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCgg                                                                            <  8:157967/147‑1 (MQ=255)                                                                         tGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTcgc                                                                      >  5:297616/1‑149 (MQ=255)                                                                           cgcgTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCgg                                                                    <  2:139293/149‑1 (MQ=255)                                                                              gtctgtctCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCt                                                                 >  6:46173/1‑149 (MQ=255)                                                                               tctgtctCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTg                                                                <  2:145640/149‑1 (MQ=255)                                                                                   tctcACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGccc                                                            >  3:118833/1‑149 (MQ=255)                                                                                   tctcACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGccc                                                            >  5:175571/1‑149 (MQ=255)                                                                                   tctcACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGcc                                                             <  4:254633/148‑1 (MQ=255)                                                                                       aCCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCctg                                                          <  6:126870/147‑1 (MQ=255)                                                                                       aCCCTGCTGGTGCAGCACCTGCCATTCATGGACATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCctgct                                                        >  8:46085/1‑149 (MQ=255)                                                                                         ccTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGt                                                                                       >  5:277390/1‑116 (MQ=255)                                                                                         ccTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGt                                                                                       <  6:277390/116‑1 (MQ=255)                                                                                         ccTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCtgctgc                                                      <  7:46085/149‑1 (MQ=255)                                                                                             ctgGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGt                                                  >  2:20314/1‑149 (MQ=255)                                                                                                gTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGt                                                                                       <  1:101102/109‑1 (MQ=255)                                                                                                gTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGt                                                                                       >  2:101102/1‑109 (MQ=255)                                                                                                gTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCg                                                <  5:46173/148‑1 (MQ=255)                                                                                                   cagcaCCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACGGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGcc                                             >  2:17622/1‑148 (MQ=255)                                                                                                   cagcaCCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGcc                                             <  4:118833/148‑1 (MQ=255)                                                                                                      caCCTGCCATTCATGGGCATTGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTgcgc                                                    >  6:153397/1‑138 (MQ=255)                                                                                                      caCCTGCCATTCATGGGCATTGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTgcgc                                                    <  5:153397/138‑1 (MQ=255)                                                                                                      caCCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAAt                                         <  1:273530/149‑1 (MQ=255)                                                                                                      caCCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAAt                                         <  5:30972/149‑1 (MQ=255)                                                                                                            ccATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCa                                     <  4:189317/147‑1 (MQ=255)                                                                                                            ccATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGc                                   <  6:75106/149‑1 (MQ=255)                                                                                                             cATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGcc                                  >  3:7180/1‑149 (MQ=255)                                                                                                             cATTCATGGGCATAGCTGAACAGTGGGCTGACCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCGGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGcc                                  >  6:8734/1‑149 (MQ=255)                                                                                                              aTTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCa                                     <  2:85189/145‑1 (MQ=255)                                                                                                              aTTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCa                                     >  1:85189/1‑145 (MQ=255)                                                                                                                      gCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGCCCGCAGCGAt                         <  1:20314/149‑1 (MQ=255)                                                                                                                            cTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGc                                                              >  6:79257/1‑106 (MQ=255)                                                                                                                            cTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGc                                                              <  5:79257/106‑1 (MQ=255)                                                                                                                            cTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGCCCGCAGCGATGTGATc                   <  6:297616/149‑1 (MQ=255)                                                                                                                            cTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGCCCGCAGCGATGTGATc                   <  4:7180/149‑1 (MQ=255)                                                                                                                               aaCAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGCCCGCAGCGATGTGATcgcg                >  5:287004/1‑149 (MQ=255)                                                                                                                               aaCAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGCCCGCAGCGATGTGATc                   <  5:283750/146‑1 (MQ=255)                                                                                                                               aaCAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGCCCGCAGCGATGTGATc                   >  6:283750/1‑146 (MQ=255)                                                                                                                                            ccTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGACCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGAt    >  3:25080/1‑148 (MQ=255)                                                                                                                                             cTTCTGGATGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCa  <  5:39670/149‑1 (MQ=255)                                                                                                                                             cTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCa  <  6:175571/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                    CGCAAGAAGTCATTCAGCTGGTGCAGACTCAGGGCTTCAGCGTCCTGATAAGGCGGCAAGCCCGGCTCGCTCTGCGCGTCTGTCTCACCCTGCTGGTGCAGCACCTGCCATTCATGGGCATAGCTGAACAGTGGGCTGCCCTTCTGGAAGTACCGAGGGCTGAACGGCTGCAGCGGGTGCTCCTGGGTCAGTGCGTGCAACAGTTGCTCGCCCGGTCTCGCGGGCTGGCCCTGCTGCGCGTCGGCCAAATGCCAGCCCGCAGCGATGTGATCGCGCAACTGGCCGATCA  >  NC_002947/5304673‑5304961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑