Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,726,809 Δ1 bp 12.3% coding (157/2574 nt) opgH ← glucans biosynthesis glucosyltransferase H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,726,8090C.12.3% 169.8 / 18.2 57coding (157/2574 nt)opgHglucans biosynthesis glucosyltransferase H
Reads supporting (aligned to +/- strand):  ref base C (22/28);  new base . (3/4);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGCCTTGCGCTTGAG  >  NC_002947/5726662‑5726957
                                                                                                                                                     |                                                                                                                                                    
gcCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACg                                                                                                                                                     >  7:277108/1‑149 (MQ=255)
      gCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCg                                                                                                                                               <  5:93413/149‑1 (MQ=255)
       cTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCgg                                                                                                                                              <  2:6560/149‑1 (MQ=255)
       cTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCgg                                                                                                                                              <  2:293955/149‑1 (MQ=255)
        tCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCGCCGAGGC‑CGTGCA‑GGCCTCggg                                                                                                                                             >  8:56327/1‑148 (MQ=255)
          gggCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCGCCGAGGCAG‑TGCA‑GGCCTCggtgg                                                                                                                                           <  7:170969/149‑4 (MQ=255)
          gggCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCGCCGAGGC‑CGTGCA‑GGCCTCggtgg                                                                                                                                           <  7:56327/149‑4 (MQ=255)
          gggCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑CGTGCA‑GGCCTCggt                                                                                                                                             <  4:301277/147‑2 (MQ=255)
           ggCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCa                                                                                                                                            >  6:90745/1‑147 (MQ=255)
           ggCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCa                                                                                                                                            <  5:90745/147‑1 (MQ=255)
               cGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTg                                                                                                                                      <  2:269687/149‑1 (MQ=255)
                         gCGTTTGATCGGAGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCAGTTCGGCTTCTTCAAGCTCGGCGGCGCTGCCAACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCAGCAGAGCTGCCGGCCGGGt                                                                                                                            >  5:218681/1‑149 (MQ=255)
                              tGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCgg                                                                                                                                              <  3:107947/126‑1 (MQ=255)
                              tGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGgc                                                                                                                        <  6:5074/148‑1 (MQ=255)
                               gATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGt                                                                                                                            >  7:16848/1‑143 (MQ=255)
                               gATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGt                                                                                                                            <  8:16848/143‑1 (MQ=255)
                                 tCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGGCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGgccgcc                                                                                                                    <  5:158033/149‑1 (MQ=255)
                                     cggGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGc                                                                                                                <  8:277108/149‑1 (MQ=255)
                                     cggGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGc                                                                                                                <  1:182367/149‑1 (MQ=255)
                                     cggGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGACCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGc                                                                                                                <  2:308629/149‑1 (MQ=255)
                                        ggCGTTCTTCAGGCACAGGCGGCCGGTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGtt                                                                                                             <  6:226089/149‑1 (MQ=255)
                                        ggCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGtt                                                                                                             <  7:235626/149‑1 (MQ=255)
                                         gCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGGTg                                                                                                            >  7:151535/1‑149 (MQ=255)
                                                 cAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGgt                                                                                                          <  2:203152/143‑1 (MQ=255)
                                                   ggCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑AGCACCGCCTCGGCAGAGCAGGCCGCCGGGTTGGCCCCCCAGCGTTGGTGTAGCTc                                                                                                   >  2:146090/1‑148 (MQ=255)
                                                        aGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCACAGCTGGCGGCCGCGTTGGCCGCCAACCGTTCGTGTAGCTCACTGaa                                                                                             >  5:131511/1‑149 (MQ=255)
                                                               cGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTg                                                                                      >  6:255493/1‑149 (MQ=255)
                                                                    ccGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGc                                                                                  <  5:255493/148‑1 (MQ=255)
                                                                               gAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCt                                                                         >  7:237056/1‑146 (MQ=255)
                                                                               gAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCt                                                                         <  8:237056/146‑1 (MQ=255)
                                                                                              ttcCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCGCGGCAGAGCTGGCGGCCGGGGTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCACCTGGCAAGCAAAGCCCCCTGCTCGTCGc                                                       >  2:177437/1‑149 (MQ=255)
                                                                                                ccAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCt                                                      >  3:131968/1‑148 (MQ=255)
                                                                                                ccAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCt                                                      >  2:84388/1‑148 (MQ=255)
                                                                                                ccAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGGTGGCCGCCAAGCGGTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCt                                                      >  5:11959/1‑148 (MQ=255)
                                                                                                 cAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCCCGGCCGCGGCAGAGCTGGCGGCCGGGTTGGCCGCCCAGCGTTGGTGTAGCTCACTGCACGCGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCt                                                      >  7:170065/1‑147 (MQ=255)
                                                                                                      cggcggCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGt                                               <  1:84388/149‑1 (MQ=255)
                                                                                                       ggcggcGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCtcgt                                                          <  3:115673/137‑1 (MQ=255)
                                                                                                       ggcggcGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGt                                               <  1:177437/148‑1 (MQ=255)
                                                                                                           gcgcTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTg                                          >  6:300018/1‑149 (MQ=255)
                                                                                                                 cccACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCACTCAGTGGTAGGTGGGCCAg                                    >  3:214181/1‑149 (MQ=255)
                                                                                                                   cACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGt                                  <  1:146090/149‑1 (MQ=255)
                                                                                                                   cACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCCTTGGTGTAGCTCACTGAACGAGGTGCACCTGGCAAGCTCAGCCCGCTGCTCGTCGCGCAGTGGTAGGTGGGCGAgg                                   >  2:269641/1‑148 (MQ=255)
                                                                                                                     cGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCACTCAGTGGTAGGTGGGCCAGGTAc                                <  4:214181/149‑1 (MQ=255)
                                                                                                                              gcgGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGc                       <  8:151535/149‑1 (MQ=255)
                                                                                                                                 ggCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGAt                    <  3:231534/149‑1 (MQ=255)
                                                                                                                                    ctgccGAGGC‑CGTGCA‑GGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCaa                         >  7:213116/4‑141 (MQ=255)
                                                                                                                                    ctgccGAGGC‑CGTGCA‑GGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCaa                         <  8:213116/138‑1 (MQ=255)
                                                                                                                                     cACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCg                >  4:134557/1‑149 (MQ=255)
                                                                                                                                       ccGAGGC‑CGTGCA‑GGCCTCGGTAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGc              >  6:142990/1‑149 (MQ=255)
                                                                                                                                       ccGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGc              >  5:45353/1‑149 (MQ=255)
                                                                                                                                               c‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGc                                       >  5:4601/1‑117 (MQ=255)
                                                                                                                                               c‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGc                                       <  6:4601/117‑1 (MQ=255)
                                                                                                                                               c‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGCCTTgcgc       >  6:77184/1‑149 (MQ=255)
                                                                                                                                                  gCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGAtt                   >  4:284/1‑135 (MQ=255)
                                                                                                                                                  gCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGAtt                   <  3:284/135‑1 (MQ=255)
                                                                                                                                                    aCGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGCCTTGCGCTTGa   <  5:300018/149‑1 (MQ=255)
                                                                                                                                                    aCGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGCCTTGCGCTTGa   <  8:170065/149‑1 (MQ=255)
                                                                                                                                                     cGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGCCTTGCGCTTGAg  <  6:11959/149‑1 (MQ=255)
                                                                                                                                                     cGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGCCTTGCGCTTGAg  <  4:131968/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGC‑C‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGCCTTGCGCTTGAG  >  NC_002947/5726662‑5726957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑