| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,907,912 | Δ1 bp | 7.5% | coding (55/1095 nt) | spuE ← | spermidine/putrescine ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,907,912 | 0 | C | . | 7.5% | 163.9 / 7.1 | 53 | coding (55/1095 nt) | spuE | spermidine/putrescine ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (22/26); new base . (2/2); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
CTTGCCTTCCAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAGCTTCGATGCCGC > NC_002947/5907766‑5908058 | cTTGCCTTCCAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGc > 2:209070/1‑147 (MQ=255) gCCTTCCAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGc < 8:243739/148‑1 (MQ=255) ccTTCCAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGt < 1:209070/149‑1 (MQ=255) ccTTCCAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGt < 7:187160/149‑1 (MQ=255) cTTCCAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTc < 7:42050/149‑1 (MQ=255) ccAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCTGGCATG‑CAGCGTcc < 6:14138/147‑2 (MQ=255) ccAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAgg > 8:162550/1‑149 (MQ=255) cAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCTGGCATG‑CAGCGTccaa < 4:278703/148‑4 (MQ=255) ggTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCcg > 8:91153/1‑149 (MQ=255) gTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCgccag > 6:227766/1‑149 (MQ=255) aGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATc < 3:35881/149‑1 (MQ=255) gAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAgg > 7:32430/1‑149 (MQ=255) aaCACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGc > 1:267840/1‑149 (MQ=255) ttGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCgg > 8:63026/1‑148 (MQ=255) gCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGaa < 5:272598/148‑1 (MQ=255) cacaGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATa < 7:162550/149‑1 (MQ=255) acaGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATAc < 5:227766/149‑1 (MQ=255) caGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACa < 4:112054/149‑1 (MQ=255) aGGCTTGATGCCCGTGGCTTTCTCGAAGACCGCGAGTGTGGTGGCGCCGATGAAGTCGGACCAGGTATAGAAGTGCAACGTCGGCGCCGCTTGGACCCT‑GCATGCCCGCCTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATa > 8:68578/1‑146 (MQ=255) gCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGaa < 7:284990/149‑1 (MQ=255) aTGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGa < 8:32430/149‑1 (MQ=255) ccGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAg > 5:49953/1‑149 (MQ=255) cGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACa < 2:267625/136‑1 (MQ=255) cGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACa > 1:267625/1‑136 (MQ=255) ggCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGGCAGCGTCAGGCCCGCGCCTGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTggg < 7:68578/149‑1 (MQ=255) gCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTgggg < 2:267840/149‑1 (MQ=255) tcGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCt > 5:62055/1‑148 (MQ=255) gAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCgccagcc > 6:303274/1‑103 (MQ=255) gAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCgccagcc < 5:303274/103‑1 (MQ=255) gAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTcac < 3:293615/149‑1 (MQ=255) tCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCCCAAt > 2:154110/1‑148 (MQ=255) gtggtgGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCtt < 4:239168/101‑1 (MQ=255) gtggtgGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCtt > 3:239168/1‑101 (MQ=255) tggtggGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTgg < 6:292261/148‑1 (MQ=255) tggtggGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTgg < 7:63026/148‑1 (MQ=255) gggCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGc < 5:253590/148‑1 (MQ=255) gggCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGc < 2:147365/148‑1 (MQ=255) gTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGcc < 7:91153/149‑1 (MQ=255) ggACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACa < 1:210917/91‑1 (MQ=255) ggACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACa > 2:210917/1‑91 (MQ=255) cAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACg > 5:135674/1‑149 (MQ=255) gTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCtt < 1:236710/75‑1 (MQ=255) gTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCtt > 2:236710/1‑75 (MQ=255) ataGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTgg > 1:135915/1‑120 (MQ=255) ataGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTgg < 2:135915/120‑1 (MQ=255) taGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAAc < 6:62055/149‑1 (MQ=255) taGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAAc > 1:68985/1‑149 (MQ=255) gcTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGc < 6:49953/149‑1 (MQ=255) cTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCGTTGCGGAATACAGATATAGACAAGTGGGGGTCCTCTCAATCAGTGCCTGGGTAGCGTCAATGCTCCCGCTCACGCAATACCGGCGCGCAACTTAACTTCGct > 5:45023/1‑148 (MQ=255) cTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCg > 2:188771/1‑147 (MQ=255) cctgACGCTGGCATG‑CAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAg > 5:33891/4‑149 (MQ=255) ctgACGCTGGCATG‑CAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAGc > 1:106348/3‑149 (MQ=255) ggACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAGCt > 6:27269/1‑149 (MQ=255) gCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAGCTTCGATGCCGc > 8:179323/1‑148 (MQ=255) | CTTGCCTTCCAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAGCTTCGATGCCGC > NC_002947/5907766‑5908058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |