Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,231,835 Δ1 bp 6.9% coding (878/1545 nt) glpD ← aerobic glycerol‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,231,8350G. 6.9% 225.5 / 10.4 72coding (878/1545 nt)glpDaerobic glycerol‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (37/29);  new base . (3/2);  total (41/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.11e-01

CGCCGAAGGGTTGTCCGACTCGTCGTTGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTCATACAGGCGCGGCACGATAATGTGG  >  NC_002947/1231690‑1231981
                                                                                                                                                   |                                                                                                                                                   
cgccgAAGGGTTGTCCGACTCGTCGTTGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCgg                                                                                                                                                  >  7:31275/1‑149 (MQ=255)
  ccgAAGGGTTGTCCGACTCGTCGTTGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGAACGGTAATTGCGACCGCGGcc                                                                                                                                                >  8:277906/1‑149 (MQ=255)
    gAAGGGTTGTCCGACTCGTCGTTGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCg‑g                                                                                                                                             <  8:31275/149‑1 (MQ=255)
    gAAGGGTTGTCCGACTCGTCGTTGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCg‑g                                                                                                                                             <  8:201901/149‑1 (MQ=255)
          ttGTCCGACTCGTCGTTGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACC‑CGGCCGCGGTcgcc                                                                                                                                       <  2:302341/149‑1 (MQ=255)
                gACTCGTCGTTGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTTGGGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTg                                                                                                                                   >  1:238585/1‑148 (MQ=255)
                 aCTCGTCGTTGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTAt                                                                                                                                <  4:145281/149‑1 (MQ=255)
                  cTCGTCGTTGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTAtt                                                                                                                               >  7:78569/1‑149 (MQ=255)
                     gtcgtTGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTccc                                                                                                                            >  1:179464/1‑149 (MQ=255)
                     gtcgtTGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTc                                                                                                                              <  8:254018/147‑1 (MQ=255)
                         ttGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGt                                                                                                                         >  7:85305/1‑148 (MQ=255)
                             acaGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAgtg                                                                                                                    >  6:307970/1‑149 (MQ=255)
                             acaGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAgtg                                                                                                                    >  2:88958/1‑149 (MQ=255)
                             acaGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGAACGCGGCCG‑GGTAGCCGCTGTATTCCCGGTCCgtg                                                                                                                    >  3:226162/1‑149 (MQ=255)
                                gCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAgtggtg                                                                                                                 <  8:78569/149‑1 (MQ=255)
                                 cGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTATTGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAgtggt                                                                                                                  <  2:31938/147‑1 (MQ=255)
                                 cGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAgtggtgg                                                                                                                >  1:31938/1‑148 (MQ=255)
                                     cGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGAt                                                                                                            >  7:267459/1‑149 (MQ=255)
                                        aCGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAg                                                                                                         >  2:89272/1‑149 (MQ=255)
                                               aGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAg                                                                                                  <  3:32063/149‑1 (MQ=255)
                                                 tAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAACGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCg                                                                                                <  1:88958/149‑1 (MQ=255)
                                                   ggTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCgg                                                                                               <  5:169866/148‑1 (MQ=255)
                                                      aTGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATTGAGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGAGGTc                                                                                             >  3:243811/1‑147 (MQ=255)
                                                       tGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTc                                                                                             >  6:306704/1‑146 (MQ=255)
                                                         cAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGa                                                                                         >  6:290563/1‑148 (MQ=255)
                                                            gATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACg                                                                                     >  6:47221/1‑149 (MQ=255)
                                                                tCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATc                                                                                                           >  8:255633/1‑123 (MQ=255)
                                                                tCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATc                                                                                                           <  7:255633/123‑1 (MQ=255)
                                                                tCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGa                                                                                  <  1:25741/148‑1 (MQ=255)
                                                                  ggCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATg                                                                                >  2:250890/1‑148 (MQ=255)
                                                                         gCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCagaaga                                                                         >  3:112928/1‑149 (MQ=255)
                                                                          cTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGAc                                                                        <  1:89272/149‑1 (MQ=255)
                                                                           tCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTGTTTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGAc                                                                        >  1:76267/1‑149 (MQ=255)
                                                                                  gtggtTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGGCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATgcgc                                                                 <  2:238585/148‑1 (MQ=255)
                                                                                     gtTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACg                                                                                     <  7:11687/125‑1 (MQ=255)
                                                                                     gtTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACg                                                                                     >  8:11687/1‑125 (MQ=255)
                                                                                              cTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCt                                                           <  6:52236/142‑1 (MQ=255)
                                                                                              cTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCt                                                           >  5:52236/1‑142 (MQ=255)
                                                                                                cGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGCCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGc                                                  <  8:267459/149‑1 (MQ=255)
                                                                                                     acgacCTTCAACATGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGa                                               <  5:71663/147‑1 (MQ=255)
                                                                                                     acgacCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGAt                                                                                 <  8:203718/113‑1 (MQ=255)
                                                                                                     acgacCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGAt                                                                                 >  7:203718/1‑113 (MQ=255)
                                                                                                     acgacCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGa                                               >  6:71663/1‑147 (MQ=255)
                                                                                                       gacCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATg                                            >  3:172045/1‑148 (MQ=255)
                                                                                                       gacCTTCAACAGGTAATGTC‑TTTCATGCTCGGTAATGCCGACCCCGCCCG‑GGTCGCAGCTGTATTCCCGGTCAGTGGTTCCGATCAGGCTAAAGCGTTCCAGATACGCGATGCAGAAAACGATGCGCTGGAATTCATTCTGAATAATg                                            >  6:274704/1‑148 (MQ=255)
                                                                                                         ccgtCAACAGGTACTGGG‑TTTCCTGCTCGGTAATGGCTACCGCGGCCG‑GGTCGCCGATGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATTCGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGATTGTAg                                         <  4:226162/146‑1 (MQ=255)
                                                                                                         ccTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAg                                         <  5:307970/149‑1 (MQ=255)
                                                                                                         ccTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAg                                         >  7:242873/1‑149 (MQ=255)
                                                                                                             cAACAGTTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCATGTCAGTGGTGCCGATCAGGGTAAAGCGCTCCAGATACGGGATGCAGAAGACGATGCGCTTGTCTTCATTCTGCAGAATGTAGGCa                                      <  1:250890/148‑1 (MQ=255)
                                                                                                             cAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGc                                       <  2:179464/147‑1 (MQ=255)
                                                                                                              aaCAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATg                                    >  2:238196/1‑149 (MQ=255)
                                                                                                                 aGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTc                                 >  6:143002/1‑149 (MQ=255)
                                                                                                                      aTGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCtt                            <  8:242873/149‑1 (MQ=255)
                                                                                                                      aTGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCtt                            >  8:202984/1‑149 (MQ=255)
                                                                                                                       tgtg‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCtt                            >  8:67650/1‑148 (MQ=255)
                                                                                                                       tgtg‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTc                           <  7:67650/149‑1 (MQ=255)
                                                                                                                            tttCCTGCACGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTCATa                        <  7:277906/148‑1 (MQ=255)
                                                                                                                                 tGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTCATACAGgcg                  >  8:199694/1‑149 (MQ=255)
                                                                                                                                  gCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGATCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTCATACAGgcgc                 >  7:70463/1‑149 (MQ=255)
                                                                                                                                      ggTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTCATACAGGCGCGGCa             >  3:85142/1‑149 (MQ=255)
                                                                                                                                        tAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTCATACAGGCGCGGCAc            >  5:14993/1‑148 (MQ=255)
                                                                                                                                         agcggCGACC‑CGGCCGCGGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTCATACAGGCGCGGCACGa          >  5:85787/4‑149 (MQ=255)
                                                                                                                                          gcggCGACC‑CGGCCGCGGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTCATACAGGCGCGGCACGa          >  5:88788/3‑148 (MQ=255)
                                                                                                                                             gCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGc                                       >  8:178238/1‑116 (MQ=255)
                                                                                                                                             gCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGc                                       <  7:178238/116‑1 (MQ=255)
                                                                                                                                             gCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTCATACAGGCGCGGCACGATAAt      <  4:85142/149‑1 (MQ=255)
                                                                                                                                             gCGACC‑CGGCCGCGGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCt                                                           <  6:169584/96‑1 (MQ=255)
                                                                                                                                             gCGACC‑CGGCCGCGGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCt                                                           >  5:169584/1‑96 (MQ=255)
                                                                                                                                               gACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTa                                          <  4:271129/111‑1 (MQ=255)
                                                                                                                                               gACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTa                                          >  3:271129/1‑111 (MQ=255)
                                                                                                                                               gACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTCATACAGGCGCGGCACGATAAtgt    >  7:75694/1‑149 (MQ=255)
                                                                                                                                                 ccGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTCATACAGGCGCGGCACGATAATGTgg  <  5:274704/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCCGAAGGGTTGTCCGACTCGTCGTTGCACAGCGGCCGAACGCCGGAGTAGGTATGCAGGATGTCGGCTT‑GGCTCAGTTGGTGGTTGAAGTGCTCGTTGACGACCTTCAACAGGTAATGTG‑TTTCCTGCTCGGTAATGGCGACCGCGGCCG‑GGTCGCCGCTGTATTCCCGGTCAGTGGTGCCGATCAGGGTAAAGCGGTCCAGATACGGGATGCAGAAGACGATGCGCTGGTCTTCATTCTGCAGAATGTAGGCATGTTCGCCTTCATACAGGCGCGGCACGATAATGTGG  >  NC_002947/1231690‑1231981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑