| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,819,789 | Δ1 bp | 8.8% | intergenic (+26/‑449) | rpoS → / → PP_1624 | RNA polymerase sigma S factor/group II intron‑encoding maturase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,819,789 | 0 | G | . | 8.8% | 174.6 / 11.0 | 57 | intergenic (+26/‑449) | rpoS/PP_1624 | RNA polymerase sigma S factor/group II intron‑encoding maturase |
| Reads supporting (aligned to +/- strand): ref base G (25/27); new base . (3/2); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGG > NC_002947/1819641‑1819933 | gaTGTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCg < 3:56336/149‑1 (MQ=255) tGTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGACAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGt > 5:136657/1‑149 (MQ=255) cTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCg > 7:215298/1‑149 (MQ=255) tGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCgg < 5:270713/149‑1 (MQ=255) aTCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑t > 1:13537/1‑149 (MQ=255) tCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTg < 4:151176/136‑1 (MQ=255) tCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTg > 3:151176/1‑136 (MQ=255) aCCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTtgtgt < 8:62717/148‑1 (MQ=255) aCCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTtgtgt < 6:201869/148‑1 (MQ=255) aCCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTtgtgt > 7:62717/1‑148 (MQ=255) aCCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCC‑GAGATCACCGGGGCG‑ttttttgt < 8:29707/147‑4 (MQ=255) ccGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTgcg > 6:248346/1‑149 (MQ=255) aCGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCTAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGc < 8:231728/149‑1 (MQ=255) cGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGgcgc > 3:80621/1‑149 (MQ=255) cGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGgcgc > 2:42457/1‑149 (MQ=255) tCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTgcgc < 2:96090/149‑1 (MQ=255) gAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATgcg < 8:215298/148‑1 (MQ=255) cgcgAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTgtgg < 6:254410/148‑1 (MQ=255) gcgAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg > 7:178822/1‑146 (MQ=255) gcgAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg < 8:178822/146‑1 (MQ=255) cgAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGtggtgg < 4:77419/149‑1 (MQ=255) ttGAAAAGAATGGTCTGTCGAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGt < 4:222530/69‑1 (MQ=255) ttGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGt > 3:222530/1‑69 (MQ=255) ttGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑ttt < 7:104559/84‑1 (MQ=255) ttGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑ttt > 8:104559/1‑84 (MQ=255) ttGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTgg > 3:299369/1‑149 (MQ=255) aaaaGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCAGCTTGGTTGTGGTGGCCAAGTGGGt > 5:152780/1‑148 (MQ=255) aaatattGGTCTGTCCAGCGAGTCATTGTTCCAGAAGCTACCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGa < 8:189073/143‑1 (MQ=255) aaaGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGa < 5:248346/149‑1 (MQ=255) aaaGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGa > 5:45525/1‑149 (MQ=255) aaGAATGGTCTGTCCATCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTCATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCAGCTTGATTGTCGTGGCCAAGAGGGTGa > 2:255454/1‑148 (MQ=25) aGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACt < 6:136657/149‑1 (MQ=255) aTGGTCTGTCCAGCGAGTCATTGTTCAAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTgg < 6:5540/149‑1 (MQ=255) gTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCTGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAgg < 4:70380/149‑1 (MQ=255) gTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAgg > 6:187763/1‑149 (MQ=255) gTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAgg < 5:209325/149‑1 (MQ=255) cTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGt > 4:205539/1‑148 (MQ=255) tCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa < 5:187763/149‑1 (MQ=255) gAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCctc < 6:152780/149‑1 (MQ=255) ccAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCgg > 7:93739/1‑149 (MQ=255) aGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGc > 7:219996/1‑148 (MQ=255) gTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTaca < 1:107857/139‑1 (MQ=255) gTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTaca > 2:107857/1‑139 (MQ=255) tagctagcGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAg > 4:65399/1‑149 (MQ=255) gctagcGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAggg < 2:13537/149‑1 (MQ=255) gcgcTGTAACAAATTACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGaa < 1:255454/148‑1 (MQ=255) gcTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAgt < 8:264686/148‑1 (MQ=255) aCAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTTTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGcc > 2:49842/1‑149 (MQ=255) aaaaaaCGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCg > 8:172568/1‑148 (MQ=255) aaaaaaCGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGGGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCgg > 5:7575/1‑149 (MQ=255) aaaaaCGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCg > 7:285434/1‑147 (MQ=255) aaaaaCGCCCC‑GAGATCACCGGGGCGTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAACGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCg > 1:124118/1‑148 (MQ=11) aaaaaCGCCCC‑GAGATCACCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg < 2:147353/82‑1 (MQ=14) aaaaaCGCCCC‑GAGATCACCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg > 1:147353/1‑82 (MQ=14) aaaaaCGCCCC‑GAGATCACCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCg > 3:150032/1‑147 (MQ=11) aaaaCGACCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAg < 3:163648/149‑1 (MQ=255) gCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAg < 1:42457/149‑1 (MQ=255) ccccGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAgg < 4:299369/149‑1 (MQ=255) | GATGTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGG > NC_002947/1819641‑1819933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |